How Enzymes Work : From Structure to Function book cover
1st Edition

How Enzymes Work
From Structure to Function

ISBN 9789814463928
Published February 5, 2015 by Jenny Stanford Publishing
234 Pages - 18 Color & 105 B/W Illustrations

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Book Description

For a long time, enzymes have been studied by measuring their activity, which has led to the advancement of "enzyme kinetics." In recent years, the mechanism of enzyme reaction has been explained in detail on the basis of the 3D structure. Genetic engineering and the 3D structural analysis of enzymes contribute to these advancements in enzymology. This book starts with an introduction to various enzymes to show how interesting enzymes are, which is followed by historical kinetic studies on enzymes and the overall and rapid-reaction kinetics. The subsequent topics describe the basics of protein structure, the control of enzyme activity, and the purification of enzymes. A case on the kinetic and structural studies of l-phenylalanine oxidase is also presented. There are many good books on enzyme kinetics, but few describe their kinetic and structural aspects. This book deals with both and contains many references that can be good sources for further reading. It is handy and is especially helpful for beginners. A number of figures, including some with stereo expression, facilitate observing the 3D structure of enzymes.

Table of Contents


Chapter One


General properties of enzyme

Examples of enzyme


Chapter Two

Overall reaction kinetics

Road to the steady state kinetics

Demonstration of the enzyme-substrate complex

Meaning of steady state

Kinetic parameters



Chapter Three

Factors affecting on the enzyme activity


Substrate concentration




Chapter Four

Effect of pH, temperature, and high pressure on the enzymatic activity

Effect of pH

Thermodynamics in the enzymatic reaction

Temperature dependence of the enzymatic reaction

Effect of pressure

Effect of temperature and pressure on α-chymotrypsin-catalyzed reaction



Chapter Five

Measurements of individual rate constants

Rapid mixing techniques

Analysis of the first-order reaction



Chapter Six

Structure of proteins

Amino Acids

Polypeptide and protein

Analysis of primary structure

Three dimensional structure

Tertiary and quaternary structures

Structural motif and loop



Chapter Seven

Active site structure

Active site and active center

Cofactor, coenzyme, prosthetic group

Search of active site

Examples of active site studies



Control of enzyme activity

Regulation by non-covalent interaction

Regulation by covalent Modification



Chapter Nine

Preparation of enzyme

Extraction of enzyme

Purification of enzyme

Purity analysis of enzyme



Chapter Ten

A case study: L-Phenylalanine ocidase (deaminating and decarboxylating)


Preparation of PAO

Catalytic properties of PAO

Molecular Properties of PAO

Physiological role of PAO




Derivation of the rate equation by King-Altman’s method

Physical constants

Conversion of units

Greek numbers and alphabet

Useful softwares and data banks

Genetic code

Solutions for problems


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Haruo Suzuki is professor emeritus at Kitasato University, Tokyo, Japan, a councilor of the Japanese Biochemical Society, and a member of the Japan Society for Bioscience Biotechnology and Agrochemistry. A biochemist, he graduated from the Department of Chemistry, Tokyo Metropolitan University, in 1966 and received his DSc from the Division of Biophysics and Biochemistry, the Graduate School of Science, University of Tokyo, in 1971. He worked as a postdoctoral fellow in the Department of Pathology, University of California at San Diego, from 1971 to 1973. He worked at the Institute for Developmental Research, Aichi Prefectural Colony, Japan, from 1973 to 1978, Kitasato University School of Medicine, Japan, from 1978 to 1994, and Kitasato University School of Science from 1994 to 2007. Prof. Suzuki’s research interests focus on the computer analysis (QM/MD) of enzyme catalysis.


"The enzyme is a classic but very important material that everybody in the field of biology, medicinal chemistry, biotechnology, and medicine must be familiar with. It is the extreme feature of protein that works in the cells. This concise book covers classic and modern enzymology and, therefore, is an excellent guide for those who possess the basic knowledge of chemistry and want to proceed to advanced courses."

—Prof. Takeshi Nishino, University of Tokyo, Japan

"This carefully written book provides very useful information on enzymes and will immensely benefit not only undergraduate and graduate students but also researchers interested in enzymes."

—Dr. Hitoshi Nakamoto, Saitama University, Japan