Hybridizing Surface Probe Microscopies: Toward a Full Description of the Meso- and Nanoworlds, 1st Edition (Paperback) book cover

Hybridizing Surface Probe Microscopies

Toward a Full Description of the Meso- and Nanoworlds, 1st Edition

By Susana Moreno-Flores, Jose L. Toca-Herrera

CRC Press

356 pages | 22 Color Illus. | 166 B/W Illus.

Purchasing Options:$ = USD
New in Paperback: 9781138374584
pub: 2018-11-21
SAVE ~$14.20
Hardback: 9781439871003
pub: 2012-11-08
SAVE ~$34.00
Currently out of stock
eBook (VitalSource) : 9780429064869
pub: 2012-11-08
from $35.50

FREE Standard Shipping!


Many books and reviews about scanning probe microscopies (SPM) cover the basics of their performance, novel developments, and state-of-the-art applications. Taking a different approach, Hybridizing Surface Probe Microscopies: Towards a Full Description of the Meso- and Nanoworlds encompasses the technical efforts in combining SPM with spectroscopic and optical complementary techniques that, altogether, provide a complete description of nanoscale and mesoscale systems and processes from corrosion to enzymatic reactions.

The book is organized into eight chapters, following a general scheme that revolves around the two main capabilities of SPM: imaging and measuring interactions. Each chapter introduces key theoretical concepts and basic equations of the particular stand-alone technique with which the scanning probe microscopies are combined. Chapters end with the SPM-technique combination and some real-world examples in which the combination has been devised or used. Most chapters include a historical review of the techniques and numerous illustrations to support key ideas and provide the reader with intuitive understanding.

To understand the limitations of any technique also means to understand how this technique works. This book has devoted a considerable amount of space in explaining the basics of each technique as they are being introduced. At the same time, it avoids explaining the particularities of each SPM-based technique and opts for a rather generalized approach. In short, the book’s focus is not on what SPM can do, but rather on what SPM cannot do and, most specifically, on presenting the experimental approaches that circumvent these limitations.

Table of Contents


Observing Nature: Sequentiality and Simultaneity

Combination Macroscopic/Microscopic: Extending Measurable Range to Better Understand the Connection between Ultrastructure and Function

Systems That Can Profit from Combined Techniques

Final Remarks


Scanning Probe Microscopy as an Imaging Tool: The Blind Microscope

Probe Imaging



What Brings Optical Microscopy: The Eyes at the Microscale

Fundamentals of Optical Microscopy

Fluorescence Microscopy: Bestowing Specificity

High-Performance Modes of Fluorescence Microscopy

Optical Microscopies: Summary

Combined OM-SPM Techniques: Eyesight to the Blind


What Brings the Scanning Near-Field Optical Microscopy: The Eyes at the Nanoscale

Fundamentals of Scanning Near-Field Optical Microscopy

Applications of SNOM


Adding Label-Free Chemical Spectroscopy: Who Is Who?

Chemical Spectroscopy

Raman (and IR) Microscopy

Applications of SPM + Raman Spectroscopy

Applications of SPM + IR Spectroscopy


Combining the Nanoscopic with the Macroscopic: SPM and Surface-Sensitive Techniques

Model-Based Surface Techniques

Fundamentals of Surface Plasmon Resonance

Fundamentals of Ellipsometry

Fundamentals of Quartz Crystal Microbalance

The Combination SPM and Model-Based Surface Techniques


Scanning Probe Microscopy to Measure Surface Interactions: The Nano Push-Puller

Force Curves: Surface Forces and More

Measuring the Probe-Sample Interaction as a Function of the Relative DisplacementQuantitative Determination of Forces: Instrument and Cantilever Calibration

The Issue of Getting Absolute Distances

Qualitative Interpretation of Force Curves

Chemical Force Microscopy

The Science of Pulling: Single Molecules or Ligand-Receptor Pairs

The Science of Pushing: Contact Nanomechanics

Mapping Interactions



Tidying Up Loose Ends for the Nanopush-Puller: Microinterferometry and the Film Balance


Fundamentals of Reflection Interference Contrast Microscopy

The Combined SPM-RICM Technique

The Film Balance and Air–Fluid Interfaces

Fundamentals of the Film Balance

The Combined AFM + Film Balance: The Monolayer Particle Interaction Apparatus (MPIA)


About the Authors

Susana Moreno Flores graduated with a degree in chemistry at the Complutense University (Madrid, Spain), where she also did her doctorate studies at the Department of Physical Chemistry. Thereafter she did postdoctoral stays at the Department of Statistical Physics of the Ecole Normale Supérieure (Paris, France), at the Max-Planck Institute of Polymer Research (Mainz, Germany) and at the Chemistry Department of the University of Basel (Basel, Switzerland), before she worked as researcher in the Biosurfaces Unit at CIC BiomaGUNE (San Sebastián, Spain). She is currently assistant professor at the Department of Nanobiotechnology of the University of Natural Resources and Life Sciences (Vienna, Austria).

José L. Toca-Herrera (Santander, Spain) is Professor of Biophysics at the University of Natural Resources and Life Sciences (BOKU-Vienna, Austria). After receiving his degree in Physics from the University of Valencia, he carried out a 12-month research training at the Max-Planck Institute for Polymer Research (Mainz) with Prof. Wolfgang Knoll. He completed his Ph.D. at the Max-Planck Institute of Colloids and Interfaces (Golm) under the supervision of Prof. Helmuth Möhwald. After several postdoctoral stays with Prof. Regine von Klitzing (TU-Berlin), Prof. Jane Clarke (University of Cambridge) and Prof. Uwe B. Sleytr (BOKU-Vienna), he joined in 2004 the Department of Chemical Engineering of the Rovira i Virgili University (Tarragona) as RyC research professor. In 2007 he moved to CIC BiomaGUNE (San Sebastián) led by Prof. Martin-Lomas as group leader. In September 2010, he joined the Department of Nanobiotechnology at BOKU (Vienna) where he leads the Laboratory of Biophysics.

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Chemistry / General
SCIENCE / Solid State Physics
SCIENCE / Spectroscopy & Spectrum Analysis