Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications: 1st Edition (Paperback) book cover

Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications

1st Edition

Edited by Perry G. Wang, Weixuan He

CRC Press

610 pages | 293 B/W Illus.

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This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.


"[This book] contains a collection of case studies of practical importance, illustrating the development, optimization, and validation of HILIC methods for particular separation problems. Most chapters are clearly written, with emphasis on the experimental detail, and contain very useful up-to-date references. Researchers in analytical and bioanalytical chemistry may find this reference book useful."

—P. Jandera, Anal Bioanal Chem (2012) 404:11–12

Table of Contents




Aqueous Normal-Phase Chromatography: The Bridge between Reversed-Phase and HILIC, Joseph J. Pesek and Maria T. Matyska

Application of the Calculated Physicochemical Property, Log D, to Hydrophilic Interaction Chromatography in the Bioanalytical Laboratory, Eugene P. Kadar, Chad E. Wujcik, and Olga Kavetskaia

HILIC Behavior of Reversed-Phase/Ion-Exchange Mixed-Mode Stationary Phases and Their Applications, Xiaodong Liu and Christopher A. Pohl

Mechanisms Controlling the Separation and Retention of Proanthocyanidins and Other Plant Polyphenols in Hydrophilic Interaction Chromatography, Akio Yanagida and Yoichi Shibusawa

Applications of Hydrophilic Interaction Chromatography to Food Analysis, Leticia Mora, Aleida S. Hernández-Cázares, M.-Concepción Aristoy, Fidel Toldrá, and Milagro Reig

Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry (HILIC-MS) of Paralytic Shellfish Poisoning Toxins, Domoic Acid, and Assorted Cyanobacterial Toxins, Carmela Dell’Aversano

The Application of HILIC for Polar Environmental Contaminants (Including Pharmaceuticals) in Aquatic Systems, Alexander L.N. van Nuijs, Isabela Tarcomnicu, Hugo Neels, and Adrian Covaci

Hydrophilic Interaction Chromatography–Mass Spectrometry: An Environmental Application, Kerry M. Peru, Sandra L. Kuchta, John V. Headley,and Allan J. Cessna

Clinical Applications of Hydrophilic Interaction Liquid Chromatography, Ping Wang

Dimethylarginine Analysis by HILIC-MS/ MS and Its Applications in Clinical Chemistry and In Vivo Animal Models, O. D’Apolito, G. Paglia, M. Zotti, F. Tricarico, L.Trabace, and G. Corso

Polar Functional Groups for HILIC Method, Zhigang Hao

Analysis of Pharmaceutical Impurities Using Hydrophilic Interaction Liquid Chromatography, Mingjiang Sun and David Q. Liu

Fast In-Process Method for the Determination of Ioversol and Related Polar Compounds by Hydrophilic Interaction Chromatography (HILIC) and UPLC, Yuming Chen, Xinqun Huang, Shaoxiong Huang, and Michael Matchett

Retention Behavior of Hydrazine Derivatives in HILIC Mode, Juris Hmelnickis, Igors Susinskis, and Kaspars Kokums

HILIC Retention Behavior and Method Development for Highly Polar Basic Compounds Used in Pharmaceutical Synthesis, Minhui Ma and Min Liu

Retention of Polar Acidic and Basic Drugs by HILIC, Mohammed Shahid Ali and Aamer Roshanali Khatri

Retention and Selectivity of Polar Stationary Phases for Hydrophilic Interaction Chromatography, Yong Guo

HILIC-MS/MS for the Determination of Polar Bioactive Substances: Representative Applications in the Fields of Pharmacokinetics and Metabolic Profiling, Yannis L. Loukas and Yannis Dotsikas

Method Development and Analysis of Mono- and Diphosphorylated Nucleotides by HILIC HPLC-ESI-MS, Samuel H. Yang, Hien P. Nguyen, and Kevin A. Schug

Hydrophilic Interaction Liquid Chromatography–Based Enrichment Protocol Coupled to Mass Spectrometry for Glycoproteome Analysis, Cosima Damiana Calvano

Development and Application of Methods for Separation of Carbohydrates by Hydrophilic Interaction Liquid Chromatography, Goran Karlsson

Hydrophilic Interaction Liquid Chromatography in the Characterization of Glycoproteins, Joanne E. Nettleship

Analysis of Protein Glycosylation and Phosphorylation Using HILIC-MS, Morten Thaysen-Andersen, Kasper Engholm-Keller,and Peter Roepstorff


About the Editors

Perry G. Wang is currently Principal Scientist at Teleflex Medical in Wyomissing, Pennsylvania.

About the Series

Chromatographic Science Series

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Pharmacy
SCIENCE / Chemistry / General
SCIENCE / Chemistry / Analytic
SCIENCE / Chemistry / Industrial & Technical