1st Edition

Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications

ISBN 9781138113398
Published May 22, 2017 by CRC Press
610 Pages 293 B/W Illustrations

USD $89.95

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Book Description

This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.

Table of Contents

Aqueous Normal-Phase Chromatography: The Bridge between Reversed-Phase and HILIC, Joseph J. Pesek and Maria T. Matyska
Application of the Calculated Physicochemical Property, Log D, to Hydrophilic Interaction Chromatography in the Bioanalytical Laboratory, Eugene P. Kadar, Chad E. Wujcik, and Olga Kavetskaia
HILIC Behavior of Reversed-Phase/Ion-Exchange Mixed-Mode Stationary Phases and Their Applications, Xiaodong Liu and Christopher A. Pohl
Mechanisms Controlling the Separation and Retention of Proanthocyanidins and Other Plant Polyphenols in Hydrophilic Interaction Chromatography, Akio Yanagida and Yoichi Shibusawa
Applications of Hydrophilic Interaction Chromatography to Food Analysis, Leticia Mora, Aleida S. Hernández-Cázares, M.-Concepción Aristoy, Fidel Toldrá, and Milagro Reig
Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry (HILIC-MS) of Paralytic Shellfish Poisoning Toxins, Domoic Acid, and Assorted Cyanobacterial Toxins, Carmela Dell’Aversano
The Application of HILIC for Polar Environmental Contaminants (Including Pharmaceuticals) in Aquatic Systems, Alexander L.N. van Nuijs, Isabela Tarcomnicu, Hugo Neels, and Adrian Covaci
Hydrophilic Interaction Chromatography–Mass Spectrometry: An Environmental Application, Kerry M. Peru, Sandra L. Kuchta, John V. Headley,and Allan J. Cessna
Clinical Applications of Hydrophilic Interaction Liquid Chromatography, Ping Wang
Dimethylarginine Analysis by HILIC-MS/ MS and Its Applications in Clinical Chemistry and In Vivo Animal Models, O. D’Apolito, G. Paglia, M. Zotti, F. Tricarico, L.Trabace, and G. Corso
Polar Functional Groups for HILIC Method, Zhigang Hao
Analysis of Pharmaceutical Impurities Using Hydrophilic Interaction Liquid Chromatography, Mingjiang Sun and David Q. Liu
Fast In-Process Method for the Determination of Ioversol and Related Polar Compounds by Hydrophilic Interaction Chromatography (HILIC) and UPLC, Yuming Chen, Xinqun Huang, Shaoxiong Huang, and Michael Matchett
Retention Behavior of Hydrazine Derivatives in HILIC Mode, Juris Hmelnickis, Igors Susinskis, and Kaspars Kokums
HILIC Retention Behavior and Method Development for Highly Polar Basic Compounds Used in Pharmaceutical Synthesis, Minhui Ma and Min Liu
Retention of Polar Acidic and Basic Drugs by HILIC, Mohammed Shahid Ali and Aamer Roshanali Khatri
Retention and Selectivity of Polar Stationary Phases for Hydrophilic Interaction Chromatography, Yong Guo
HILIC-MS/MS for the Determination of Polar Bioactive Substances: Representative Applications in the Fields of Pharmacokinetics and Metabolic Profiling, Yannis L. Loukas and Yannis Dotsikas
Method Development and Analysis of Mono- and Diphosphorylated Nucleotides by HILIC HPLC-ESI-MS, Samuel H. Yang, Hien P. Nguyen, and Kevin A. Schug
Hydrophilic Interaction Liquid Chromatography–Based Enrichment Protocol Coupled to Mass Spectrometry for Glycoproteome Analysis, Cosima Damiana Calvano
Development and Application of Methods for Separation of Carbohydrates by Hydrophilic Interaction Liquid Chromatography, Goran Karlsson
Hydrophilic Interaction Liquid Chromatography in the Characterization of Glycoproteins, Joanne E. Nettleship
Analysis of Protein Glycosylation and Phosphorylation Using HILIC-MS, Morten Thaysen-Andersen, Kasper Engholm-Keller,and Peter Roepstorff

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Perry G. Wang is currently Principal Scientist at Teleflex Medical in Wyomissing, Pennsylvania.


"[This book] contains a collection of case studies of practical importance, illustrating the development, optimization, and validation of HILIC methods for particular separation problems. Most chapters are clearly written, with emphasis on the experimental detail, and contain very useful up-to-date references. Researchers in analytical and bioanalytical chemistry may find this reference book useful."
—P. Jandera, Anal Bioanal Chem (2012) 404:11–12