1st Edition

Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications

Edited By Perry G. Wang, Weixuan He Copyright 2011
    616 Pages 293 B/W Illustrations
    by CRC Press

    610 Pages 293 B/W Illustrations
    by CRC Press

    This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.

    Aqueous Normal-Phase Chromatography: The Bridge between Reversed-Phase and HILIC, Joseph J. Pesek and Maria T. Matyska
    Application of the Calculated Physicochemical Property, Log D, to Hydrophilic Interaction Chromatography in the Bioanalytical Laboratory, Eugene P. Kadar, Chad E. Wujcik, and Olga Kavetskaia
    HILIC Behavior of Reversed-Phase/Ion-Exchange Mixed-Mode Stationary Phases and Their Applications, Xiaodong Liu and Christopher A. Pohl
    Mechanisms Controlling the Separation and Retention of Proanthocyanidins and Other Plant Polyphenols in Hydrophilic Interaction Chromatography, Akio Yanagida and Yoichi Shibusawa
    Applications of Hydrophilic Interaction Chromatography to Food Analysis, Leticia Mora, Aleida S. Hernández-Cázares, M.-Concepción Aristoy, Fidel Toldrá, and Milagro Reig
    Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry (HILIC-MS) of Paralytic Shellfish Poisoning Toxins, Domoic Acid, and Assorted Cyanobacterial Toxins, Carmela Dell’Aversano
    The Application of HILIC for Polar Environmental Contaminants (Including Pharmaceuticals) in Aquatic Systems, Alexander L.N. van Nuijs, Isabela Tarcomnicu, Hugo Neels, and Adrian Covaci
    Hydrophilic Interaction Chromatography–Mass Spectrometry: An Environmental Application, Kerry M. Peru, Sandra L. Kuchta, John V. Headley,and Allan J. Cessna
    Clinical Applications of Hydrophilic Interaction Liquid Chromatography, Ping Wang
    Dimethylarginine Analysis by HILIC-MS/ MS and Its Applications in Clinical Chemistry and In Vivo Animal Models, O. D’Apolito, G. Paglia, M. Zotti, F. Tricarico, L.Trabace, and G. Corso
    Polar Functional Groups for HILIC Method, Zhigang Hao
    Analysis of Pharmaceutical Impurities Using Hydrophilic Interaction Liquid Chromatography, Mingjiang Sun and David Q. Liu
    Fast In-Process Method for the Determination of Ioversol and Related Polar Compounds by Hydrophilic Interaction Chromatography (HILIC) and UPLC, Yuming Chen, Xinqun Huang, Shaoxiong Huang, and Michael Matchett
    Retention Behavior of Hydrazine Derivatives in HILIC Mode, Juris Hmelnickis, Igors Susinskis, and Kaspars Kokums
    HILIC Retention Behavior and Method Development for Highly Polar Basic Compounds Used in Pharmaceutical Synthesis, Minhui Ma and Min Liu
    Retention of Polar Acidic and Basic Drugs by HILIC, Mohammed Shahid Ali and Aamer Roshanali Khatri
    Retention and Selectivity of Polar Stationary Phases for Hydrophilic Interaction Chromatography, Yong Guo
    HILIC-MS/MS for the Determination of Polar Bioactive Substances: Representative Applications in the Fields of Pharmacokinetics and Metabolic Profiling, Yannis L. Loukas and Yannis Dotsikas
    Method Development and Analysis of Mono- and Diphosphorylated Nucleotides by HILIC HPLC-ESI-MS, Samuel H. Yang, Hien P. Nguyen, and Kevin A. Schug
    Hydrophilic Interaction Liquid Chromatography–Based Enrichment Protocol Coupled to Mass Spectrometry for Glycoproteome Analysis, Cosima Damiana Calvano
    Development and Application of Methods for Separation of Carbohydrates by Hydrophilic Interaction Liquid Chromatography, Goran Karlsson
    Hydrophilic Interaction Liquid Chromatography in the Characterization of Glycoproteins, Joanne E. Nettleship
    Analysis of Protein Glycosylation and Phosphorylation Using HILIC-MS, Morten Thaysen-Andersen, Kasper Engholm-Keller,and Peter Roepstorff


    Perry G. Wang is currently Principal Scientist at Teleflex Medical in Wyomissing, Pennsylvania.

    "[This book] contains a collection of case studies of practical importance, illustrating the development, optimization, and validation of HILIC methods for particular separation problems. Most chapters are clearly written, with emphasis on the experimental detail, and contain very useful up-to-date references. Researchers in analytical and bioanalytical chemistry may find this reference book useful."
    —P. Jandera, Anal Bioanal Chem (2012) 404:11–12