Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish.
- Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging.
- Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system.
- Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography and super-resolution imaging.
- Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior.
- Addresses the impact on discovery, such as cellular function as implicated in human disease and translational medicine, for example in cancer diagnosis.
Table of Contents
Section I: Overview of Imaging Methods and Instrumentation
Chapter 1: Fluorescence microscopy techniques
MS Ozturk and R Prevedel
Chapter 2: Intra-vital microscopy
M Perro, JG Goetz, and A Peixoto
Chapter 3: An introduction to live-cell super-resolution imaging
S Culley, PM Pereira, RF Laine, and R Henriques
Chapter 4: Endoscopic Optical Coherence Tomography: Technologies and Applications,
D Li and X Li
Chapter 5: Bioluminescence
M Conway, T Xu, A Brumbaugh, A Young, D Close, and S Ripp
Chapter 6: Macroscopic Fluorescence Imaging,
Chapter 7: Optical Coherence Tomography
R Dsouza and SA Boppart
Chapter 8: Multiscale Photoacoustic Imaging
T Wang and S Hu
Chapter 9 : Fluorescence Lifetime: Techniques, Analysis & Applications in the Life Sciences
J Chacko, MAK Sagar and KW Eliceiri
Section II: Imaging cellular behavior
Chapter 10: Imaging cell metabolism
R Cao, H Wallrabe, KH Siller, and A Periasamy
Chapter 11: Intravital imaging of cancer cell migration in vivo
D Entenberg, MH Oktay, and J Condeelis
Chapter 12: Imaging cellular signaling in vivo using fluorescent protein biosensors
CA Reissaus, RN Day, and KW Dunn
Chapter 13: Imaging cell adhesion and migration
C Mondal, J Di Martino and JJ Bravo-Cordero
Chapter 14: Imaging the living eye
BT Soetikno, L Beckmann, and HF Zhang
Section III: Whole-organ and whole-organism imaging
Chapter 15: Heart imaging
L Sacconi and C Crocini
Chapter 16: Visualizing hepatic immunity through the eyes of intravital microscopy
MA Freitas-Lopes, MM Antunes, R Carvalho-Gontijo, E Carvalho, and GB Menezes
Chapter 17: Optical imaging of the mammalian oviduct in vivo,
S Wang and IV Larina
Chapter 18: Immune system imaging
MJ. Hickey and MU Norman
Chapter 19: Brain imaging in live mice
M Fukuda, K Ozawa, and H Hirase
Chapter 20: Live imaging of zebrafish
Y Wan, PJ Keller, and B Höckendorf
Chapter 21: Whole-body fluorescence imaging in cancer research
MV Shirmanova, DV Yuzhakova, MM Lukina, AI Gavrina, AV Meleshina, IV Turchin, and EV Zagaynova
Chapter 22: Large-scale fluorescence imaging in neuroscience
Margarida Barroso is a Professor at the Department of Molecular and Cellular Physiology, Albany Medical College (Albany, NY).
Xavier Intes is a Professor in the Biomedical Engineering Department and Co-Director of the Center for Modeling, Simulation and Imaging for Medicine (CeMSIM),at Rensselaer Polytechnic Institute (Troy, U.S.).