1st Edition

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

By Alexander D. Hyatt, Bryan Eaton Copyright 1992
464 Pages
by CRC Press

464 Pages
by CRC Press

This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The... Read more
Immuno-Gold Electron Microscopy for Virus Diagnosis: Immune Complexing (E.L. Palmer and M.L. Martin). Solid-Phase Immune Electron Microscopy of Virus Preparations (R.G. Milne). Gold Labeling in Negative Stain Immune Electron Microscopy (P.J.C. Dopping-Hepenstal and J.E. Beesley). The Grid-Cell-Culture Technique (A.D. Hyatt and B.T. Eaton). Use of Microspheres for Immune Scanning Electron Microscopy (J.P. Andersson, U.G. Andersson, and A. Sonnerborg). Immuno-Gold Electron Microscopy in Virus Research: The Colloidal Gold Probe, An Overview of Its Application in Viral Cytochemistry (S. Garzon and M. Bendayan). Detection of Viral and Cellular Structures by Post-Embedding Immunocytochemistry (G.N. Williams and P. Faulkner). Multiple Labeling of Thin Sections (M. Nielsen and L. Bastholm). Immuno-Gold Replica Technique for Study of Virus-Infected Cells (K. Mannweiler, H. Hohenberg, G. Rutter, and W. Bohn). Detection and Analysis of Viral Proteins by Immuno-Gold Labeling (K.G. Murti). Cryo-Sectioning (H.K. Hagler). Improvements of Immuno Dection Using Ultra-Small Gold Probes and Cryosections (J.L.M. Leunissen and P. van de Plas). Freeze Substitution in Virus Research: A Preview (H. Schwartz, H. Hohenberg, and B.M. Humbel). Ultrastructural In Situ Hybridization Using Immuno-Gold (G.L. Bassell and R.H. Singer). Use of Colloidal Gold and Silver Enhancement in Scanning Electron Microscopy (A.D. Hyatt and S.M. Brookes).

Biography

Alexander D. Hyatt, Bryan Eaton