Theory and Practice
A complete and balanced overview of all aspects of immunocytochemistry is presented providing a clear understanding of their impact on experiment. All available techniques and many diagnostic and research applications are included, as well as practical step-by-step instructions for carrying out recommended methods. Intended for the novice as well as the experienced researchers.
Table of Contents
Antibodies and Antisera. Fixation and Tissue Pretreatment. Immunocytochemical Detection Systems. Section Pretreatment, Epitope Demasking, and Methods for Dealing with Unwanted Staining. Associated Methods. Index.
Lars-Inge Larsson, D.M.Sc., is Professor of Histochemistry and Head of the Department of Molecular Cell Biology, State Serum Institute, Copenhagen, Denmark. Dr. Larsson received his D.M.Sc. in 1975 at the Department of Histology, University of Lund, Sweden. From 1976 to 1982 he held an appointment as Associate Professor at the Institute of Medical Biochemistry, University of Aarhus, Denmark and was appointed Professor of Histochemistry in 1982. Between 1982 and 1987 he directed the first MRC Unit in Denmark, jointly supported by the Danish MRC and Cancer Society. In 1988 he constructed and was appointed head of the new Department of Molecular Cell Biology at the State Serum Institute in Copenhagen. Dr. Larsson is the author of over 200 scientific articles and books. He received the Boehringer-Ingelheim award in 1982, the Mack-Forster award (European Society for Clinical Investigation) in 1986, and the Anders Jahre and Lundbeck awards in 1987. He is, according to a recent survey made by the Institute of Scientific Information (lSI), among the 250 most cited primary authors in all scientific disciplines. His main area of interest is concerned with studies of neurohormonal peptides and biogenic amines and with the development of histochemical and biochemical methods for the study of these substances. He is the author of a very large number of original articles and reviews on immunocytochemical methodology.