In Situ Hybridization in Electron Microscopy: 1st Edition (Hardback) book cover

In Situ Hybridization in Electron Microscopy

1st Edition

By Gerard Morel, Annie Cavalier, Lynda Williams

CRC Press

472 pages

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Hardback: 9780849300448
pub: 2001-01-30
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pub: 2001-01-30
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Description

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:

° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)

° hybridization prior to embedding (pre-embedding)

° hybridization on ultrathin sections of frozen tissue (frozen tissue method).

For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.

The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

Table of Contents

General Introduction

Abbreviations

Probes

Principles of Methodology

Sample Preparation

Post-Embedding Technique

Pre-Embedding Technique

Frozen Tissue Technique

Semithin Sections

Controls and Problems

Examples of Results

Appendices

Equipment

Reagents

Glossary

Index

About the Series

Methods in Visualization

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
SCI017000
SCIENCE / Life Sciences / Cytology
SCI047000
SCIENCE / Microscopes & Microscopy
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology