In Situ Hybridization in Light Microscopy: 1st Edition (Hardback) book cover

In Situ Hybridization in Light Microscopy

1st Edition

By Gerard Morel, Annie Cavalier

CRC Press

344 pages

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Hardback: 9780849307034
pub: 2000-09-28
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Description

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

Table of Contents

General Introduction

Abbreviations

Probes

Tissue Preparation

Pretreatments

Hybridization

Revelation of Hybrids

Counterstaining and Modes of Observation

Controls and Problems

Typical Protocols

Examples of Observation

Appendices

Glossary

Index

About the Series

Methods in Visualization

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Subject Categories

BISAC Subject Codes/Headings:
SCI017000
SCIENCE / Life Sciences / Cytology
SCI047000
SCIENCE / Microscopes & Microscopy
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology