In Situ Hybridization in Light Microscopy  book cover
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1st Edition

In Situ Hybridization in Light Microscopy




ISBN 9780849307034
Published September 28, 2000 by CRC Press
344 Pages

 
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Book Description

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

Table of Contents

General Introduction
Abbreviations
Probes
Tissue Preparation
Pretreatments
Hybridization
Revelation of Hybrids
Counterstaining and Modes of Observation
Controls and Problems
Typical Protocols
Examples of Observation
Appendices
Glossary
Index

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