In Vitro Plant Breeding  book cover
1st Edition

In Vitro Plant Breeding

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ISBN 9781560229087
Published November 19, 2001 by CRC Press
182 Pages

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Book Description

Create improved crops with these techniques for plant cell culture!

This comprehensive book presents the basic concepts and applied techniques of plant cell and tissue culture. More and more, commercial plant breeding and development employs these methods to protect crops from weather, pests, and disease. Covering the history of in vitro breeding as well as emerging research trends, In Vitro Plant Breeding offers specific techniques for crop improvement and breeding.

Designed as a text for undergraduate students, In Vitro Plant Breeding presents the theory of tissue culture as well as practical techniques. Its step-by-step instructions and clear illustrations facilitate learning and laboratory work.

In Vitro Plant Breeding gives in-depth information and the latest research on the vital concepts and techniques of in vitro breeding, including:

  • applications of plant tissue culture
  • morphogenesis and organogenesis
  • micropropagation
  • producing haploid plants in vitro
  • in vitro pollination and fertilization
  • problems of embryo culture
  • somatic hybridization
  • protoplast technology
  • selection of desirable traits
  • cryopreservation and plant breeding
  • micrografting
This helpful book is plentifully illustrated with examples, schematic descriptions, and tables to make the concepts clear and easy to learn. In Vitro Plant Breeding is an essential resource.

Table of Contents


  • Foreword
  • Preface
  • Chapter 1. Introduction
  • Types of In Vitro Culture
  • Applications of Plant Tissue Culture
  • Chapter 2. Morphogenesis/Organogenesis
  • Introduction
  • Plant Growth
  • Cellular Differentiation
  • Morphogenesis
  • Chapter 3. Micropropagation
  • Definition
  • Stages in Micropropagation
  • Commercial Micropropagation
  • Applications of Micropropagation
  • Chapter 4. Haploid Plant Production In Vitro
  • Anatomy of Anther
  • Anther Culture
  • Androgenesis
  • Chapter 5. In Vitro Pollination and Fertilization
  • Development of Female Gametophyte
  • Pollination
  • Fertilization
  • Embryo Culture
  • Chapter 6. Somatic Hybridization Using Protoplast Technology
  • Introduction
  • Uses of Protoplast Technology
  • Obtaining Protoplasts
  • The Culture of Protoplasts
  • The Cytoplasmic Genomes
  • Common Potential of Protoplast Fusion
  • Chapter 7. Cell Culture and Selection of Desirable Traits
  • Selection of Naturally Occurring Variants in Culture
  • General Selection Strategies
  • Chapter 8. In Vitro Mutagenesis
  • Types of Mutagens
  • Determining the Type and Suitable Concentration of Mutagens
  • The Choice of Plant Tissues for In Vitro Mutagensis
  • Chapter 9. The Origin, Nature, and Significance of Variation in Tissue Culture
  • Introduction
  • The Basis of Somaclonal Variations
  • Causes of Somaclonal Variations
  • Use of Somaclonal Variation in Breeding
  • Prevention of Somaclonal Variation
  • Chapter 10. Cryopreservation and Plant Breeding
  • Introduction
  • Theory and Technology
  • Cryopreservation Protocols for Cold-Hardy and Non-Cold-Hardy Species
  • Storage and Thawing
  • Equipment for Cryopreservation
  • Practical Issues and Strategies Toward Improved Cryoprotection
  • Chapter 11. In Vitro Micrografting
  • Definition of Micrografting
  • Analysis of Compatibility and Incompatibility Phenomena
  • Chapter 12. In Vitro Flowering: Its Relevance to Plant Breeding
  • Factors Influencing In Vitro Flowering
  • Plant Growth Regulators
  • Mineral Nutrients and Other Medium Components
  • Explant, Light, and Other Variables
  • Application of In Vitro Flowering to Plant Breeding
  • Chapter 13. In Vitro Tuberization
  • Introduction
  • Factors Controlling Microtuber Production
  • Practical Aspects of In Vitro Tuberization
  • Chapter 14. Molecular Plant Breeding
  • Types of Molecular Markers
  • Major Objectives of Molecular Breeding
  • Applications of Molecular Markers in Plant Breeding
  • Case Study: Application of Molecular Markers in Barley (Hordeum vulgare) Breeding
  • References
  • Index

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