1st Edition

Insect Transgenesis
Methods and Applications

ISBN 9780367398385
Published December 18, 2020 by CRC Press
424 Pages

USD $74.95

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Book Description

Imagine scientists controlling the transmission of certain diseases through the genetic modification of mosquitoes. Eradicating harmful insects without the use of pesticides. Or increasing the fertility of some insects who in turn eat harmful arthropods or even a plant pathogen. Those are just a few of the real-world applications of insect transgenesis, which offers substantial benefits to humankind-whether it be in improving agricultural productivity or reducing the spread of insect-vectored diseases.
Insect Transgenesis: Methods and Applications is the first publication to describe in a comprehensive manner the various methodologies available, possible applications, and the risk assessment and regulatory issues involved in this fascinating area of research.
Divided into several areas of interest, the book starts with an overview of the history and methodology of insect gene transfer. The book then examines gene targeting by homologous recombination and recombination systems, and systems for transgenic selection, including visible eye color markers, chemical resistance, and fluorescent proteins. Other sections consider the use of various vector systems to integrate DNA into a host genome or to express foreign genes in a host organism.
The work concludes with strategies for the use of transgenic insects, including examples for agricultural pests and vectors of disease. Of particular interest are the final chapters that discuss risk assessment considerations and governmental regulatory procedures for the transport and release of transgenic insects.

Table of Contents

SECTION I — INTRODUCTION -- Chapter 1 An Introduction to the History and Methodology of Insect Gene Transfer/Alfred M. Handler -- SECTION II — GENE TARGETING -- Chapter 2 Targeted Transformation of the Insect Genome/Paul Eggleston and Yuguang Zhao -- Chapter 3 Site-Specific Recombination for the Genetic Manipulation of Transgenic Insects/Yikang S. Rong and Kent G. Golic -- SECTION III —TRANSGENIC SELECTION -- Chapter 4 Eye Color Genes for Selection of Transgenic Insects/Abhimanyu Sarkar and Frank H. Collins -- Chapter 5 Green Fluorescent Protein (GFP) as a Marker for Transgenic Insects/Stephen Higgs and David L. Lewis -- Chapter 6 Resistance Genes as Candidates for Insect Transgenesis/Richard H. ffrench-Constant and Mark Q. Benedict -- SECTION IV — VIRAL VECTORS -- Chapter 7 Pantropic Retroviral Vectors for Insect Gene Transfer/Jane C. Burns -- Chapter 8 Densonucleosis Viruses as Transducing Vectors for Insects/Jonathan Carlson, Boris Afanasiev, and Erica Suchman -- Chapter 9 Sindbis Virus Expression Systems in Mosquitoes: Background, Methods, and Applications Ken E. Olson -- Chapter 10 Retrotransposons and Retroviruses in Insect Genomes/Christophe Terzian, Alain Pelisson, and Alain Bucheton -- Chapter 11 Polydnaviruses and Insect Transgenic Research/Bruce A. Webb -- SECTION V — TRANSPOSABLE ELEMENT VECTORS -- Chapter 12 Hermes and Other hAT Elements as Gene Vectors in Insects/Peter W. Atkinson and David A. O ’Brochta -- Chapter 13 Genetic Engineering of Insects with mariner Transposons/David J. Lampe, Kimberly K. O. Walden, John M. Sherwood, and Hugh M. Robertson -- Chapter 14 The TTAA-Specific Family of Transposable Elements: Identification, Functional Characterization, and Utility for Transformation of Insects/Malcolm J. Fraser, Jr. -- SECTION VI — SYMBIONT VECTORS -- Chapter 15 Wolbachia as a Vehicle to Modify Insect Populations/Steven P. Sinkins and Scott L O 'Neill -- Chapter 16 Bacterial Symbiont Transformation in Chagas Disease Vectors/Charles B. Beard, Ravi V. Durvasula, and Frank F. Richards -- SECTION VII — STRATEGIES, RISK ASSESSMENT, AND REGULATION -- Chapter 17 The Application of Transgenic Insect Technology in the Sterile Insect Technique/Alan S. Robinson and Gerald Franz -- Chapter 18 Control of Disease Transmission through Genetic Modification of Mosquitoes/Anthony A. James -- Chapter 19 Deploying Transgenic Arthropods in Pest Management Programs: Risks and Realities/Marjorie A. Hoy -- Chapter 20 Regulation of Transgenic Arthropods and Other Invertebrates in the United States/Orrey P. Young, Shirley P Ingebritsen, and Arnold S. Foudin -- Index.

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Alfred M. Handler, Anthony A. James