1st Edition

Intracellular Ribozyme Applications Principles and Protocols

Edited By LA. Couture, J.J. Rossi Copyright 1999
    300 Pages
    by Taylor & Francis

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    In this definitive guide to intracellular ribozyme applications, researchers from both industry and academia review the most recent pharmaceutical, therapeutic, and biotechnological applications of ribozymes. Leading scientists from around the world present the latest principles on the application of ribozyme technology to the inhibition of gene expression in living cells. The authors are experts in the field and describe, in detail, protocols for monitoring ribozyme activity, for expressing ribozymes from DNA vectors, for delivering ribozymes to cells, and for in vitro selection of improved ribozymes. The book provides a forward-looking view of ribozyme technology for alteration of gene expression in plants and in treatment of retroviral and genetic diseases in man.

    Section 1. Selection. Section 2. Kinetics. Section 3. Expression. Secion 4. Delivery. Section 5. Dection. Section 6. Pharmackodynamics. Section 7. Applications.


    LA. Couture, J.J. Rossi