1 Introduction
2 Absorption of Light
3 Instrumentation
4 Emission and Excitation Spectra
5 Polarization/Anisotropy
6 Time-Resolved Fluorescence
7 Phasors
8 Quantum Yields and Quenching
9 Förster Resonance Energy Transfer (FRET)
10 Fluorescence Microscopy
11 Fluorophores
12 Intrinsic Protein Fluorescence
13 Appendix: Rogue’s Gallery of Fluorescence Artifacts and Errors
Biography
David M. Jameson is professor in the Department of Cell and Molecular Biology at the John A. Burns School of Medicine, University of Hawaii at Manoa, and has previously served there as professor and chairman in the Department of Biochemistry and Biophysics. He earned a PhD in biochemistry from the University of Illinois at Urbana-Champaign, where his graduate thesis advisor was Professor Gregorio Weber. Prior to his move to the University of Hawaii, he was an assistant professor in the Department of Pharmacology at the University of Texas Southwestern Medical Center at Dallas and a postdoctoral fellow at the University of Illinois at Urbana-Champaign and at the CNRS, University of Paris-South in Orsay, France. Dr. Jameson is the co-organizer of the International Weber Symposia on Innovative Fluorescence Methodologies in Biochemistry and Medicine, which have been held every three years since 1986. He was the recipient of the 2004 Gregorio Weber Award for Excellence in Fluorescence Theory/Application.
