This book provides a practically directed basis for carrying out immunocytochemical methods with enough theoretical information to allow a newcomer to understand the whys and wherefores of the technique. It explains tissue preparation, cytological preparations and new methods of multiple staining.
1. Introduction 2. Production of Antibodies 3. Preparation of Tissue for Immunocytochemistry 4. Visualizing the End-product of Reaction 5. Non-specific Staining due to Tissue Factors 6. Methods 7. Testing Antibodies: Specificity and Essential Controls 8. Increasing Sensitivity and Enhancing Standard Methods 9. Multiple Immunostaining 10. Immunocytochemistry for the Transmission Electron Microscope 11. In Vitro Methods for Testing Antigen–Antibody Reactions 12. Applications of Immunocytochemistry