1st Edition

Ion and Molecule Transport in Lysosomes

Edited By Bruno Gasnier, Michael X. Zhu Copyright 2020
252 Pages 24 Color & 8 B/W Illustrations
by CRC Press

251 Pages 24 Color & 8 B/W Illustrations
by CRC Press

251 Pages 24 Color & 8 B/W Illustrations
by CRC Press

Lysosomes are key subcellular organelles that regulate the cell function. Many of the essential activities of the cell are dependent on lysosomes. Dysfunction is linked to multiple diseases - storage disorders, neurodegeneration, immunological diseases and cancer. This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract... Read more
Preface

Editors

Contributors

1. Endosomal and Lysosomal Electrophysiology

Xiaoli Zhang, Mingxue Gu, Meiqin Hu, Yexin Yang, and Haoxing Xu

2. Chloride Transport across the Lysosomal Membrane

Sonali Saha, Anja Blessing, and Thomas J. Jentsch

3. Endolysosomal Patch Clamping: Approaches to Measure Vesicular Ion Channel Activities

Cheng-Chang Chen, Christian Grimm, Christian Wahl-Schott and Martin Biel

4. TRPML Subfamily of Endolysosomal Channels: Concepts and Methods

Nicholas E. Karagas, Morgan A. Rousseau, and Kartik Venkatachalam

5. Investigating the Role of Two-Pore Channel 2 (TPC2) in Zebrafish Neuromuscular Development

Sarah E. Webb, Jeffrey J. Kelu, and Andrew L. Miller

6. Functional Study of Lysosomal Nutrient Transporters

Xavier Leray, Corinne Sagné, and Bruno Gasnier

7. Lysosomal Vitamin B12 Trafficking

Sean Froese and Matthias R. Baumgartner

8. Detection of Lysosomal Membrane Permeabilization

Anne-Marie Ellegaard, Line Groth-Pedersen, and Marja Jäättelä

9. Rapid Isolation of Lysosomes from Cultured Cells Using a Twin Strep Tag

Jian Xiong, Jingquan He, Michael X. Zhu, and Guangwei Du

10. A Transcriptomic Analysis and shRNA Screen for Intracellular Ion Channels and Transporters Regulating Pigmentation

Donald C. Koroma, Salwa Y. Hafez, and Elena Oancea

Index

Biography

Bruno Gasnier is CNRS Research Director, Group Leader at CNRS/Paris Descartes.

Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston.