1st Edition

Laboratory Methodology in Biochemistry Amino Acid Analysis and Protein Sequencing

By C. Fini Copyright 1989
    275 Pages
    by CRC Press

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    Provides information on methodologies and techniques concerning the biochemical laboratory, as well as improvements or advancements made on existing methodologies. Original methodologies for the purification of biological macromolecules and methodologies for metabolic pathways and enzyme kinetics are covered. The application of biochemical and biophysical methodologies for the structural and dynamic characterization of biological macromolecules is considered. The elaboration of automated systems for biochemical research and computer programs for the management and processing of experimental data are both reviewed. Development of instruments and equipment for biochemical research is also presented.

    1. A Short History of Protein Sequence Analysis 2. Structural Determination of Covalently Modified Peptides by Combined Mass Spectrometry and Gas Phase Microsequencing 3. Amino Acid Analysis Using the Dimethylaminoazobenzene Sulfonyl Chloride (DABS-CI) Precolumn Derivatization Method 4. Amino Acid Analysis: Protocols, Possibilities, and Pretensions 5. Application of 4-N, N-Dimethylaminoazobenzene-4'-Isothiocyanate (DABITC) to the Structure Determination of Peptides and Proteins 6. High-Performance Liquid Chromatography (HPLC): A Versatile Tool in Peptide and Protein Chemistry 7. Computational Analysis of Protein Sequencing Data 8. Computers as Tools in Protein Sequencing 9. Mapping of Ligand Binding Sites, Epitopes, and Posttranslational Modifications on Protein Sequences-Tubulin as an Example 10. ASP-N Endoproteinase, A New Specific Tool in Protein Sequencing 11. Enzymatic Digestion of Proteins and HPLC Peptide Isolation in the Subnanomole Range 12. HPLC and Hormone Purification: Application to Atrial Natriuretic Factor 13. Electroblotting-A Method for Protein Purification for NH2-Terminal and Internal Microsequencing 14. Methods for Determination of Protein Sequences by Fast Atom Bombardment Mass Spectrometry