Life in the Frozen State: 1st Edition (Hardback) book cover

Life in the Frozen State

1st Edition

Edited by Barry J. Fuller, Nick Lane, Erica E. Benson

CRC Press

704 pages | 106 B/W Illus.

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Hardback: 9780415247009
pub: 2004-05-10
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pub: 2004-05-10
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While it is barely 50 years since the first reliable reports of the recovery of living cells frozen to cryogenic temperatures, there has been tremendous growth in the use of cryobiology in medicine, agriculture, horticulture, forestry, and the conservation of endangered or economically important species.

As the first major text on cryobiology in the genomic era, Life in the Frozen State describes the current understanding of how living cells and complex organisms survive very low temperatures. Leading world experts combine fundamental theory and practice across a spectrum of species and applications to evaluate how cryobiology can benefit humanity. Chapters encompass disciplines ranging from mathematical modeling and biophysics, to the molecular biology of stress gene expression and the clinical banking of cells and tissues.

This book provides a unique opportunity to explore the subject in a multidisciplinary context, which has historically been the key to realizing some of the most exciting advances in low temperature research.


  • Integrates fundamental theory and practice across a broad range of species and applications

  • Discusses cryobiology within a multidisciplinary context

  • Emphasizes how the current knowledge of cryobiology can be applied to benefit humanity through health care and conservation
  • Reviews

    "In recommending this book, suffice it to say that the contributions, without exception, are well referenced; the production by CRC Press is of high quality with clear print, excellent figures and a good index. I recommend that ….it be on the shelves of all polar libraries and biologists working on low temperature systems from cells to ecosystems."

    Antarctic Science, volume 17, 2005

    Table of Contents


    Principles of Cryobiology, Peter Mazur

    The Water to Ice Transition - Implications for Living Cells, Ken Muldrew, Jason Acker, Gloria Elliott, and Locksley McGann


    Life in the Polar Terrestrial Environment with a Focus on Algae and Cyanobacteria, Josef Elster and Erica Benson

    Microbial Life in Permafrost: Extended Time in Extreme Conditions, Monica Ponder, Tatiana Vishnivetskaya, John McGrath, and James Tiedje

    Adaptation of Higher Plants to Freezing, Roger Pearce

    Oxidative Stress in the Frozen Plant: A Free Radical Point of View, Erica Benson and David Bremmer

    Physiology, Biochemistry, and Molecular Biology of Vertebrate Freeze Tolerance: The Wood Frog, Kenneth B. Storey and Janet M. Storey


    Preservation of Fungi and Yeasts, C. Shuhui Tan and Cor van Ingen

    Cryo-conserving Algal and Plant Diversity: Historical Perspectives and Future Challenges, Erica Benson

    Plant Cryopreservation, Akira Sakai

    The Early History of Gamete Cryobiology, Stanley P. Leibo

    Cryobiology of Gametes and the Breeding of Domestic Animals, Alban Massip, Stanley P. Leibo, and Elizabeth Blesbois

    Cryopreservation as a Supporting Measure in Species Conservation: "Not the Frozen Zoo", Amanda R. Pickford and William V. Holt

    Cryopreservation of Gametes and Embryos of Aquatic Species, Tiantian Zhang

    Fundamental Issues for Cell-line Banks in Biotechnology and Regulatory Affairs, Glyn Stacey


    Mechanisms of Injury Caused by in vivo Freezing, Nathan E. Hoffman and John C. Bischof

    Cryopreservation in Transfusion Medicine and Haematology, Andreas Sputtek and Rebekka Sputtek

    Cryopreservation of Human Gametes and Embryos, Barry Fuller, Sharon Paynter, and Paul Watson

    The Scientific Basis for Tissue Banking, Monica Wusteman and Charles J. Hunt

    Engineering Desiccation Tolerance in Mammalian Cells: Tools and Techniques, Jason P. Acker, Tani Chen, Alex Fowler, and Mehmet Toner

    Stabilization of Cells During Freeze-Drying: The Trehalose Myth, John H. Crowe, Lois M. Crowe, Fern Tablin, William Wolkers, Ann E. Oliver, and Nelly M. Tsvetkova

    Vitrification in Tissue Preservation: New Developments, Mike J. Taylor, Yin C. Song, and Kelvin G.M. Brockband


    The Future of Cryobiology, Nick Lane, Barry Fuller, and Erica Benson

    Subject Categories

    BISAC Subject Codes/Headings:
    MEDICAL / Reproductive Medicine & Technology
    SCIENCE / Life Sciences / Botany
    SCIENCE / Life Sciences / Cytology