Although the role of liposomes in drug targeting has been discussed extensively in several reviews and books, there has been no comprehensive coverage of related methodology. This book constitutes the first attempt to put together all aspects of lipsome technology as applied to medical sciences. Volume II describes procedures for the entrapment of a number of drugs, including genetic material, into selected types of lipsomes.
Table of Contents
1. Model Studies for Drug Entrapment and Lipsome Stability 2. Preparation of Lipsomes Entrapping Cancer Chemotherapeutic Agents for Experimental In Vivo and In Vitro Studies 3. Liposome Encapsulation of Biological Response Modifiers: Activation of Tumoricidal Macrophages 4. Preparation of Liposomes for Use as Drug Carriers in the Treatment of Leishmaniasis 5. Incorporation of Factor VIII into Liposomes 6. Acetyl Glyceryl Ether Containing Liposomes 7. The Labeling of Liposomal Membranes with Radioactive Technetium 8. Methodological Considerations for the Use of Liposomes in Diagnostic Imaging 9. Liposomes and Carriers of Immunogenic Determinants 10. Incorporation of Membrane Proteins in Liposomes and their Use in immunoassay 11. Theoretical and Practical Consideration in Preparing Lipsomes for the Purpose of releasing Drug in Response to Changes in Temperature and pH 12. Experimental Procedures with Liposomes to Insert Macromolecules and Macromolecular Complexes into Muscle Cells in Culture 13. Entrapment of Genetic Material into Liposomes and Delivery to Cells 14. Entrapment of Genetic Materials into Liposomes and their Delivery to Plant Protoplasts 15. Entrapment of Recombinant DNA in Liposomes and its Transfer and Expression in Eukaryotic Cells
Gregory Gregoriadis, Ph.D., is a senior member of staff at the Medical Research Council's Clinical Research Centre, Harrow, Middlesex, England. He received his first degree in Chemistry from the University of Athens and his M.Sc. and Ph.D. in Biochemistry from McGill University. He has carried out research in as diverse fields as the metabolism of trace metals and the interaction of plasma glycoproteins with hepatic receptors. Since 1970 he has worked, published, and lectured extensively on the targeting of drugs via liposomes. Dr. Gregoriadis' interest in drug targeting is reflected in his founding in 1978 the Gordon Research Conference Series on "Drug Carriers in Biology and Medicine" of which he was the first Chairman and in 1981 the NATO Advanced Studies Institute Series "Targeting of Drugs" of which he is the Director. He has also been the editor of Drug Carriers in Biology and Medicine and the senior editor of Liposomes in Biological Systems and Targeting of Drugs.