Liposomes in Gene Delivery: 1st Edition (Paperback) book cover

Liposomes in Gene Delivery

1st Edition

By Danilo D. Lasic

CRC Press

320 pages

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Paperback: 9780849331091
pub: 1997-03-13
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Description

Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. Liposomes in Gene Delivery covers both-molecular biologists will gain a basic knowledge of lipids, liposomes, and other gene delivery vehicles; lipid and drug delivery scientists will better understand DNA, molecular biology, and DNA manipulation. Topics include an introduction to nucleic acids, a theoretical description of DNA, recombinant technology, lipids and liposomes, stability and interaction properties of lipids and liposomes, complexation of lipids and liposomes with DNA plasmids, gene expression of genosomes in various models, structure-activity relationships, and transfection models. This is an excellent introductory text for graduate students, scientists, and researchers in molecular and cell biology, genetics, biochemistry, physical chemistry, colloid science, pharmacology, molecular science, and medicine.

Table of Contents

Introduction

Gene Therapy

Strategies in Gene Delivery

Disease Applications of Gene Therapy

Genetic Diseases

Cancer

Infectious Diseases

Neurological Diseases

Cardiovascular Diseases

Other Diseases

Nucleic Acids

DNA Structure and Conformation

DNA Stability and Interactions

Theoretical Description of DNA

DNA Condensation

Recombinant DNA Technology

Gene Expression

Promoters and Enhancers

Introns and PolyA Tails

Complementary DNA

Cytoplasmic Gene Expression

Reporter Genes

Vectors

Gene Delivery

Naked DNA

Viral Systems

Nonviral Delivery Systems

Liposomes

Lipids and Liposome Composition

Cationic Lipids

Electrostatic Properties of Charged Lipids and Liposomes

Cationic Liposomes

Liposome Preparation Methods

Mechanism of Vesicle Formation

Liposome Stability

Characterization and Quality Control

Interactions of Liposomes with Cells and In Vivo

Toxicity or Safety of Cationic Liposomes

Liposomes and Cell Membranes

Liposome Applications

Genosomes (DNA-Lipid Complexes)

Reactivity and Stability

Structure of Cationic Lipid-DNA Complexes

Characterization

Pharmacokinetics and Biodistribution

Toxicity

Genosomes for Gene Delivery and Transfection

Expression In Vitro

Gene Transfection and Expression In Vivo

Parenteral Applications of Genosomes

Pulmonary Delivery

Other Delivery Routes

Genosome Administration into Humans

Structure-Activity Relationships

Structure-Activity Relationships of Cationic Lipids on the Molecular Level

Structure-Activity Relationships on the Colloidal Level

Mechanism of Transfection

Transfection In Vitro

Transfection In Vivo

Factors and Steps That Determine Transfection

Other Carrier Systems and Artificial Virus

Polyelectrolytes

Combination Complexes

Liposomes in the Delivery of Antisense Oligonucleotides

Introduction

Antisense Oligonucleotides: Structure and Mechanism of Action

Liposomes for the Delivery of Antisense Oligonucleotides

An Overview of Some of the Results

Ribozymes

Delivery of Proteins

Perspectives and Conclusion

Cationic Liposomes

Anionic Liposomes

Sterically Stabilized Liposomes

Conclusion and Afterword

References

Appendix A: Some Fundamental Concepts

Appendix B: Periodic System of Elements

Appendix C: Short Tutorial on Different Units

Appendix D: Geometric Parameters of Liposomes and DNA

Appendix E: Abbreviations

Appendix F: Glossary

Index

Subject Categories

BISAC Subject Codes/Headings:
MED071000
MEDICAL / Pharmacology