Various methodologies designed to study cell walls are compiled in this book.
Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.)
Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry.
Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
Preface Cell Wall-Morphology and Chemical Composition Carbohydrates Cellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated Polysaccharides Glycoproteins Structural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like Proteins Enzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and Ribonucleuses Lipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and Tryphine Lignin and Other Phenolic Substances-Lignins, Phenolic Acids and Amides Mineral Substances-Silica, Calcium, and Boron Light Microscopic Cytochemistry Introduction Tissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of Materials Stains and Staining-Introduction, Control Procedures, Incubation of Materials Mounting of Stained Materials Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing Procedures Procedures for the Cytochemical Localizations of Different Cell Wall Substances Carbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharides Proteins-Non-Specific Cell Wall Proteins, Enzymic Proteins Lignins Sporopollenins Cutins & Suberin Minerals Fluorescent Microscopic Cytochemistry Introduction Fluorescent Cytochemical Methods Cell Wall Carbohydrates Cell Wall Proteins Lignin Ferulic Acid Sporopollenin Cutin and Suberin Transmission Electron Microscopic Cytochemistry Introduction Specimen Preparation Electron Microscopic Staining TEM Cytochemical Methods Cell Wall Carbohydrates Cell Wall Proteins Cutin and Suberin Lectin Cytochemistry Introduction Procedures Involving Lectin Probes Immunocytochemistry Introduction Basics of Immunocytochemistry-Immunization, Storing and Purifying Antibodies, Labeling of Antibodies, Tissue Preparation, Immunolabeling, Mounting of Sections Methods in Immunocytochemistry-Methods in Antibody Production and Tissue Preparation, Application of Immunocytochemistry to Study Cell Wall Components Literature Cited (at the end of each Chapter)