Methods in Cyanobacterial Research

Chapter 1: Evaluating the polyphasic approach in cyanobacterial taxonomy

Sagarika Pal, Naresh Kumar, Harsh Pant, Lira A. Gaysina and Prashant Singh

Chapter 2: Estimation of growth and photosynthetic pigments in cyanobacteria

Pankaj K. Maurya, Sapna Tiwari, Soumila Mondal, Anjali Gupta and Shailendra P. Singh

Chapter 3: Quantitative estimation of total carbohydrate, protein and lipid contents in cyanobacteria

Pankaj K. Maurya, Sapna Tiwari, Soumila Mondal, Anjali Gupta, Vinod Kumar and Shailendra P. Singh

Chapter 4: Isolation and purification of phycobiliproteins

Jyoti Jaiswal, Sonal Mishra, Neha Kumari, Varsha K. Singh and Rajeshwar P. Sinha

Chapter 5: Methods for estimation of oxidative stress indices in cyanobacteria

Vinod Kumar, Anjali Gupta, Soumila Mondal, Sapna Tiwari and Shailendra P. Singh

Chapter 6: Estimation of glutamine synthetase enzyme

Sonal Mishra, Neha Kumari, Varsha K. Singh and Rajeshwar P. Sinha

Chapter 7: Estimation of nitrate reductase enzyme

Sonal Mishra, Prashant R. Singh, Niharika Sahu and Rajeshwar P. Sinha

Chapter 8: Estimation of nitrogenase enzyme activity in cyanobacteria

Sonal Mishra, Amit Gupta, Ashish P. Singh and Rajeshwar P. Sinha

Chapter 9: Isolation and purification of cyanobacterial toxins

Sonal Mishra and Rajeshwar P. Sinha

Chapter 10: Isolation and purification of scytonemin

Neha Kumari, Sonal Mishra, Jyoti Jaiswal, Prashant R. Singh and Rajeshwar P. Sinha

Chapter 11: Isolation and purification of mycosporine-like amino acids

Sonal Mishra, Neha Kumari, Jyoti Jaiswal and Rajeshwar P. Sinha

Chapter 12: Protein estimation in cyanobacterial samples

Sonal Mishra, Jyoti Jaiswal and Rajeshwar P. Sinha

Chapter 13: Proteomic analysis of cyanobacteria employing 2-dimensional gel electrophoresis (2-DE)

Garvita Singh, Preeti Agarwal, Pritam Kaur, Akanksha Madan and Rajeshwar P. Sinha

Chapter 14: LC-MS/MS based proteomics study of cyanobacteria

Soumila Mondal, Subodh Kumar, Phulen Sarma, Bikash Medhi, Anjali Gupta, Sapna Tiwari, Pankaj K. Maurya and Shailendra P. Singh

Chapter 15: Monitoring photosynthetic performance in cyanobacteria by a modulated fluorometer

Sapna Tiwari, Pankaj K. Maurya, Soumila Mondal, Anjali Gupta, Donat-P. Häder and Shailendra P. Singh

Chapter 16: Reactive oxygen species measurement in cyanobacteria using 2′,7′- dichlorodihydrofluorescein diacetate (DCFH-DA) dye and fluorescence microscope or flow cytometer

Soumila Mondal, Anjali Gupta, Sapna Tiwari, Pankaj K. Maurya, Priyul Pandey and Shailendra P. Singh

Chapter 17: Flow cytometry-based methods for estimating DNA content and live-dead cells in cyanobacteria

Soumila Mondal, Anjali Gupta, Sapna Tiwari, Rinkesh Gupta and Shailendra P. Singh

Chapter 18: Genomic DNA and RNA extraction from cyanobacteria

Anjali Gupta, Soumila Mondal, Sapna Tiwari, Donat-P. Häder, Rajeshwar P. Sinha and Shailendra P. Singh

Chapter 19: Detection and quantification of DNA damage in cyanobacteria by immunodot- blot assay

Prashant R. Singh, Jainendra Pathak, Rajneesh, Deepak Kumar, Amit Gupta, Ashish P. Singh, Varsha K. Singh and Rajeshwar P. Sinha

Chapter 20: Engineering cyanobacteria for the synthesis of novel products

Rungaroon Waditee-Sirisattha and Hakuto Kageyama

Chapter 21: Sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria

Soumila Mondal, Bhaskar Sharma, Abhisek Majumdar, Sapna Tiwari, Pankaj K. Maurya, Anjali Gupta, Shailendra P. Singh and Prabhakar Singh

Chapter 22: Cyanobacterial biofertilizer production technology

Sonal Mishra and Rajeshwar P. Sinha

Chapter 23: Biomass composition measurement and intracellular flux analyses for insights into cyanobacterial metabolism

Piyush Pachauri, Preeti Rai and Shireesh Srivastava

Chapter 24: In silico genome assembly and functional annotation

Pannaga P. Jutur, Ashish Kumar, Mohammed Rehmanji, Rabinder Singh, Asha A. Nesamma

Index

Biography

Dr. Shailendra P. Singh is an Assistant Professor at the Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India. He earned his PhD from the Department of Biology, Friedrich-Alexander University, Erlangen-Nuremberg, Germany.

Prof. Dr. Rajeshwar P. Sinha, DAAD Fellowship Awardee and Fellow, Society for Applied Biotechnology, India, is a Professor of Molecular Biology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India.

Prof. Donat-P. Häder is the past-Director of the Botanical Institute and held the chair of Ecophysiology of Plants at the Friedrich-Alexander University, Erlangen-Nürnberg, Germany. He was also the director of the Botanical Garden.