Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.
1. The Use of Recombinant DNA Technology to Produce Genetically Modified Plants 2. Production and Analysis of Plant Mutants, Emphasizing Arabidopsis thaliana 3. DNA Sequence Organization and Gene Copy Number Determination 4. Isolation and Characterization of Plant DNAs 5. Isolation and Characterization of Plant mRNA 6. Procedures for Introducing Foreign DNA into Plants 7. Vectors for Plant Transformation 8. Construction of l Clone Banks 9. Transient Analysis of Gene Expression in Plant Cells 10. Techniques for Isolating and Characterizing Plant Transcription Promoters, Enhancers, and Terminators 11. Use of Xenopus Oocytes to Monitor Plant Gene Expression 12. In Situ Localization of Specific mRNAs in Plant Tissues 13. Immunological Methods for Assessing Protein Expression in Plants 14. In Situ Immunocytochemical Localization of Plant Proteins 15. Accessing Computer Software for Molecular Biology 16. Sequence Similarity Searches, Multiple Sequence Alignments and Molecular Tree Building 17. DNA Mapping in Plants 18. Random Amplified Polymorphic DNA -RAPD Analysis 19. Detection and Characterization of Plant Pathogens 20. Screening for Inoculant-Quality Strains of Rhizobia 21. Isolation and Characterization of Plant Growth-Promoting Rhizobacteria