Methods in Practical Laboratory Bacteriology: 1st Edition (Paperback) book cover

Methods in Practical Laboratory Bacteriology

1st Edition

By Henrik Chart

CRC Press

176 pages

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Description

The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature.

Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.

Table of Contents

Bacterial Fractionation and Membrane Protein Characterization, H. Chart

Lipopolysaccharide: Isolation and Characterization, H. Chart

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) for the Separation and Resolution of Bacterial Components, H. Chart

Reaction of Antibodies with Bacterial Components Using Immunoblotting, H. Chart

The Use of Enzyme-Linked Immunosorbent Assay (ELISA) in Bacteriology, H. Chart

Environmental Regulation of Bacterial Characteristics, P. Stevenson and E. Griffiths

Testing for Resistance to Antimicrobial Drugs, J.A. Frost

Pulsed-Field Gel Electrophoresis of Bacterial DNA, M.E. Kaufmann and T.L. Pitt

Extraction and Fingerprinting of Bacterial Plasmids, N. Woodford, A.P. Johnson, and E.J. Threlfall

Non-Radioactive Digoxigenin-Labeled DNA Probes, A. Thomas

Ribotyping of Bacterial Genomes, M.E. Kaufmann, D.G. Pitcher, and T.L. Pitt

Appendix

References

Sources of Equipment and Chemicals

Glossary

Index

Subject Categories

BISAC Subject Codes/Headings:
SCI043000
SCIENCE / Research & Methodology
SCI045000
SCIENCE / Life Sciences / Biology / Microbiology