1st Edition

Microarray Methods and Protocols





ISBN 9781420046656
Published January 20, 2009 by CRC Press
216 Pages 105 B/W Illustrations

USD $185.00

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Book Description

A Step-by-Step Guide to Present and Future Uses of Microarray Technology

Microarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts in both academia and industry, Microarray Methods and Protocols is a turn-by-turn roadmap through the processes necessary to perform a successful microarray experiment.

This easy to use book addresses the fundamental aspects of preparing and processing microarrays and bead arrays, labeling, and detection. It also includes a detailed How it Works section that discusses the underlying principles of a number of techniques. Troubleshooting guides offer additional advice for the successful performance of more than 100 protocols in 10 chapters that cover work involving nucleic acids, proteins, carbohydrates, and lectins.

--Concise and Well-Organized--

With a focus on the preparation and use of microarrays of biomedical relevance, the text describes a variety of microarray formats useful in the assessment of human disease and in genomic and proteomic research. This authoritative resource provides detailed information regarding sample preparation, labeling, array construction processes, substrate chemistry, array printing, and quality control.

Originating with the glass microscope slide and biochip, microarray technology is now pressing onward into the nanotechnology frontier. This book is the all-inclusive manual scientists need to take microarray research to the next level of discovery.

Table of Contents

Introduction to Microarray Technologies, Robert S. Matson

Nucleic Acid Sample Preparation, Robert S. Matson

Solid-Phase Substrates for Nucleic Acid Microarrays, Robert S. Matson

Protein Sample Preparation for Microarrays, Robert S. Matson

Solid-Phase Chemistries for Protein Microarrays, Robert S. Matson

Protein Microarrays: The Link between Genomics and Proteomics, Persis P. Wadia and David B. Miklos

Bead Arrays: An Introduction to Multiplexed Bead-Based Assays for Proteins, Yong Song

Carbohydrate Arrays, Denong Wang

Lectin Microarrays, Masao Yamada

Printing Methods, Todd Martinsky

Appendix A: Microarray Reagent, Materials, and

Equipment Sources

Appendix B: Image Analysis

Index

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Reviews

The book will be useful for researchers in protein chemistry and biochemistry, molecular biologists, and teachers and students of medical schools and universities specializing in microarray methods.
--Doctor of Biological Sciences, G. Ya. Wiederschain.
Biochemsitry - Moscow. 2010, VOL 75, No 9,1182