Taylor & Francis
276 pages | 58 B/W Illus.
Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology.
The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation.
Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536
"With a collection of interesting case studies and a hands-on approach, this survey of experimental and data analysis methods and techniques in microarray technology takes the needs of the novice into consideration along with the curiosity of the practitioner in applications research." Book News Inc
1. Introduction to Microarray Technology 2. Immunoprecipitation with Microarrays to Determine the Genome-Wide Binding Profile of a DNA-Associated Protein 3. Array-Based Comparative Genomic Hybridization (Array CGH) as a Tool for Solving Practical Biological and Medical Questions 4. Use of SNPs Arrays : Design, Tools and Applications 5. In vitro Analysis of Gene Expression 6. The Analysis of Cellular Transcriptional Response at the Genome Level : Two Case Studies with Relevance to Bacterial Pathogenesis 7. Functional Annotation of Microarray Experiments 8. Microarray Technology in Agricultural Research 9. Protein Microarrays Protocol 1. Microarray Printing - Standard Operating Procedure for QIAGEN OPERON Oligonucleotide Arrays Protocol 2. E. coli RNA Extraction Protocol 3. Indirect Labeling of First-strand cDNA Probes Protocol 4. Pre-Hybridization and Washing Protocol for UltraGAP Slides Protocol 5. ChIP Procedure Protocol 6. Array CGH Protocol 7. In vitro Analysis of Gene Expression Using Run-off Microarray Analysis of Gene Expression (ROMA) Appendix 1. Notes on Printing Glass DNA Microarray Slides Appendix 2. Comparative Genomics : The Nature of CGH Analysis and Data Interpretation Appendix 3. Useful Links Appendix 4. Table 7