1st Edition

Microbial Biofilm Dynamics Contemporary Approaches, Models and Analytical Tools

232 Pages 26 Color & 8 B/W Illustrations
by CRC Press

232 Pages 26 Color & 8 B/W Illustrations
by CRC Press

This book explores the dynamics of microbial biofilms, examining their role in both oral and systemic diseases, emphasizing developmental models, and presenting various characterization and detection methodologies. Divided into three sections, the introductory section covers fundamental concepts, including microbial biofilm understanding, the critical role of the extracellular matrix,... Read more

Chapter 1. Understanding microbial biofilms: characteristics, formation stages, and mechanisms of communication and immune system escape

 

Douglas Roberto Monteiroa, Ashutosh Kumar Shuklab

 

2. Biofilm extracellular matrix

Monica Moreno de Carvalho; Vitória Parmejane de Oliveira; Ana Cláudia Okamoto, Celso Koogi Sonoda, Aline Satie Takamiya

 

3. Mechanisms of antimicrobial resistance in biofilms

Laís Salomão Arias, Diego Romário da Silva, Isabela Cunha Lopes, Danielle Ferreira Sobral de Souza

 

4. Biofilms in the context of oral diseases

Laís Salomão Arias, Patrícia de Assis da Silva, Gabriely Pereira da Silva, Alexandre Henrique de Souza, Ana Auxiliadora de Matos dos Santos

 

5. Biofilms of medical significance in and around humans

 

Helen Laking, William Johnston, John P. Butcher, Ryan Kean, & Gordon Ramage

 

6. Development of novel antimicrobial therapeutic strategies for biofilm control

 

Liliana Fernandes, Maria Elisa Rodrigues and Fernanda Gomes

 

7. Exploring the spectrum of biofilm complexity

Daniela Araújo, Ricardo Oliveira, Ana Maria Campos, Maria Margarida Barros, Andreia S. Azevedo,

Ana Barbosa, Laura Cerqueira, Nuno F. Azevedo, Sónia Silva, Carina Almeida, Joana Castro

 

8. Laboratory models for biofilm studies

Joana Castro, Ricardo Oliveira, Ana Maria Campos, Maria Margarida Barros, Andreia S. Azevedo,

Ana Barbosa, Laura Cerqueira, Nuno F. Azevedo, Sónia Silva, Carina Almeida, Daniela Araújo

           

9. Quantification of microbial biofilm biomass

Douglas Roberto Monteiro, Ana Carolyna Becher Roseno, Natália Pereira Ribeiro, Thayse Yumi Hosida, Juliano Pelim Pessan

 

10. Quantification of cultivable biofilm cells: counting colony-forming units

Douglas Roberto Monteiro, Aline Satie Takamiya, Natália Pereira Ribeiro, Ana Carolyna Becher Roseno, Rosana Leal do Prado, Juliano Pelim Pessan

 

11. Analytical tools for assessing metabolic activity in biofilms

           

Caio Sampaio, Leonardo Antônio de Morais, Luigi Pedrini Guisso, Lucas Fernando de Oliveira Tomaz Ferraresso, Douglas Roberto Monteiro, Juliano Pelim Pessan, Alberto Carlos Botazzo Delbem, Thayse Yumi Hosida

 

12. Technical approaches to biofilm matrix analysis

Bruna Gonçalves; Isabel Carvalho; Débora Castro; Sónia Silva

 

13. Omics techniques (genomics, transcriptomics, proteomics and metabolomics) in the study of oral and systemic biofilms

Rogério de Castilho; Gladiston William Lobo Rodrigues; Laura Cesário Oliveira; Natalia Amanda Gomes;

Ana Paula Fernandes Ribeiro; Yuri Gabriel Chamorro de Moraes; Nayara Gabriely Dourado

 

14. Flow-cytometry analysis in biofilm research

Rocio C. Barajas-Ornelas, Hilda C. Leyva Sánchez, and Mario Pedraza-Reyes

 

15. Imaging techniques in biofilm research

           

Anju Rana, Dhruv Ahuja

 

16. Electrochemical detection of biofilms

           

Muhammad Ikram, Hussam Ibrahim, Amina Akbar Kalyar, Sikander Ameer, and *Mazhar Sher

 

17. Machine learning approaches in biofilm research: An overview

           

Eswar Rao Tatta and Ranjith Kumavath

 

           

 

 

 

Biography

Dr. Ashutosh Kumar Shukla is a Professor of Physics at Ewing Christian College, Prayagraj, a constituent college of the University of Allahabad, India. His research interest is primarily based on the applications of spectroscopy techniques, including ESR spectroscopy, in different fields. Dr. Shukla has successfully completed research projects funded by the University Grants Commission, New Delhi, and presented his research findings at different international events. He has been awarded the bilateral exchange fellowship and visiting fellowship of the Indian National Science Academy, New Delhi, in addition to research fellowships from the Council of Science and Technology, Lucknow, and the Council of Scientific and Industrial Research, New Delhi. Dr. Shukla is on the review panel and editorial boards for international journals. With more than twenty-two years of teaching experience, he has to his credit journal publications, textbooks, and more than thirty edited volumes published by reputed international publishers.

Dr. Douglas Roberto Monteiro is an Assistant Professor at the Department of Diagnosis and Surgery, School of Dentistry, Araçatuba, São Paulo State University (UNESP), Brazil. He completed his doctoral degree (2012) and post-doctoral studies (2016) from the same institution, having developed part of his doctoral project at the Department of Biological Engineering of the University of Minho, Braga, Portugal (2010–2011). He has published over 85 research articles and 35 book chapters. He has also recently been listed as one of the top 2% of scientists, according to Stanford University (Elsevier BV 2021–2024), USA. So far, 7 MSc and 3 Ph.D. theses have been completed under his guidance. His research areas include microbial biofilms of interest in the medical and dental fields, fungal infections caused by Candida species, alternative antimicrobial agents, and nanotechnology.