Preface
Samples and Sampling Methods for the Study of Microbial Diversity from Soil and Water
Physicochemical Characterization of Soil and Water Samples
Microbiological Characterization of Soil and Water Samples
Cultivation, Isolation, and Pure Culture of Bacteria and Archaea
Short-term and Long-term Preservation of Microorganisms at Extremely Low Temperatures
Long-term Preservation of Microorganisms using Freeze-drying
Biochemical Characterization of Bacteria
Tests for Screening: Aminopeptidase, KOH Test, and Antimicrobial Resistance
Bacterial DNA Extraction
A Detailed Method of Bacterial DNA Extraction using Sonication
DNA Quantification and Agarose Gel Electrophoresis
Amplification of the 16S rRNA using Polymerase Chain Reaction (PCR)
PEG-NaCl Purification of PCR Products: Principles, Protocols, and Applications
16S rRNA Gene Sequencing Method and Bioinformatic Analysis
Software Tools for Sequence Analysis and Identification
Deposition of Sequence Data in INSDC, ENA, and DDBJ Repositories
Deposition of Pure Cultures in International Culture Collections
Primer Design for Polymerase Chain Reactions (PCRs) and Sequencing PCRs
Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Microbial Gene Detection
Multilocus Sequence Typing (MLST) for Microbial Identification and Classification
Quantitative Polymerase Chain Reaction (qPCR) Protocol and Applications
Whole Genome Sequencing (WGS) of Bacteria
Bacterial and Archaeal Genome Annotation
Denaturing Gradient Gel Electrophoresis (DGGE) Protocol and its Applications in Microbial Ecology
Index
Biography
Dr. Bhagwan (MSc, PhD, MCA) is an eminent researcher in Microbiome Diversity and Microbiome. After completing his postdoctoral fellowship at BRIC-NCCS (Pune), he joined Savitribai Phule Pune University, often called "the Oxford of the East," as an Assistant Professor. He is an Associate Professor at the Yenepoya Research Centre, Yenepoya (Deemed to be University) in Mangalore, India. His current specialization is Computational Biology and Evolutionary Microbiology.
He serves as the expert for the European Commission, the French Foundation for Medical Research (France), the National Research and Development Agency (Chile), Certified Educator and Ambassador for GPTZero (USA), and the Academic Advisor for Fengkai Group Co., Limited (Hong Kong) for various international and national agencies. Since 2022, he has consistently been ranked on the AD Scientific Index (five times, ID: 4509536). His research is featured by Nature and The Times of India.
