Microbiological Examination of Water and Wastewater: 1st Edition (Hardback) book cover

Microbiological Examination of Water and Wastewater

1st Edition

By Maria Csuros

CRC Press

324 pages

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Hardback: 9781566701792
pub: 1999-03-26
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Microbiological tests have proven to be an indispensable part of environmental contaminant detection. It has also been tremendously difficult to find a comprehensive training manual and laboratory manual for those procedures.

Microbiological Examination of Water and Wastewater now provides that much-needed resource for laboratory trainees and environmental professionals alike.

An all-inclusive guide to applications and techniques of microbiological testing, Microbiological Examination of Water and Wastewater includes coverage of General Microbiology, Environmental Microbiology, Environmental Microbiology Laboratory, plus Techniques and Methods in Routine Environmental Microbiology Laboratory.

By exploring the fundamentals of microbiology, as well as microbial metabolism, growth, control, and classification, trainees will better understand the purpose and manner of microbiological examination. Those details also make Microbiological Examination of Water and Wastewater ideal as a standard guidebook for laboratories, water and wastewater treatment plants, and the communities they serve.

Table of Contents

Scope and History of Microbiology

Microbes and Microbiology

Brief History of Microbiology

The Golden Age of Microbiology


Modern Developments in Microbiology

Naming and Classifying of Microorganisms

The Diversity of Microorganisms

Procaryotic Cell

Introduction of Procaryotic and Eucaryotic Cells

The Procaryotic Cell

Structures External to the Cell Walls

The Cell Wall

Structures Internal to the Cell Wall

Microbial Metabolism

Meaning of Metabolism

Metabolic Pathways


Nutritional Classification of Organisms

Energy Production of Organisms

Carbohydrate Catabolism

Microbial Growth and its Control

Microbial Growth

Physical Requirements

Chemical Requirement

Control of Microbial Growth

Physical Methods of Microbial Control

Chemical Methods of Microbial Control

Microorganisms in the Environment

Biochemical Cycles

Microorganisms Found in Air

Microorganisms in Soil

Aquatic Microbiology

Water Purification

Sewage Treatment

Bioremediation of Organic Contaminants


Application of Bioremediation

Microbiological Quality of Environmental Samples

Monitoring Microbiological Quality

Introduction to Microbiological Parameters Regarding to the Sanitary Quality of Environmental Samples

Pathogenic Microorganisms

Iron and Sulfur Bacteria



Regulations for Drinking Water Quality


Surface Waters


Soil, Sediment


Safety in Environmental Microbiology Laboratory

Laboratory Facilities

Laboratory Safety Considerations

Summarized Safety Check List for Environmental Microbiology Laboratories

Laboratory Quality Assurance and Quality Control


Requirement for Facilities and Personnel

Quality Control of Laboratory Equipments and Instruments

Quality Control of Laboratory Supplies

Quality Control of Laboratory Pure Water

Analytical Quality Control Procedures

Records and Data Reporting

Interlaboratory Quality Control

Collecting and Handling Environmental Samples for Microbiological Examination


Sample Collection from Different Sources

Sample Identification

Sample Transportation, Preservation, and Holding Time

Laboratory Equipments and Supplies in Environmental Microbiology Laboratory

Laboratory Equipments

Laboratory Glassware

Chemicals, Reagents

Culture Media

Culture Media and Culture

Bacterial Growth in Media

Direct Measurement of Bacterial Growth


Plate Counts

Membrane Filter (MF) Technique

Most Probable Number (MPN) Method

Staining Procedures

Direct Microscopic Counts

Estimation of Bacterial Numbers by Indirect Methods


Biochemical Reactions and Enzymatic Tests

Rapid Identification Systems (Multitest Systems)

Methods for Analysing Microbiological Quality of the Environment

Methods and Techniques

Rapid Detection Methods

Methods for Microbiological Examination of Recreational Waters

Detection of Pathogenic Bacteria

Detection of Soil Microorganisms

Heterotrophic Plate Counts


Pour Plate Method

Determination of Total Coliform


Membrane Filter Method (MF)

Most Probable Number (MPN) Method

Determination of Klebsiella

Application for Soil, Sediment, and Sludge Samples

Analytical Quality Control

Determination of Fecal Coliform

Definition of the Fecal Coliform Group

Membrane Filter Method

Delayed Incubation MF Method

The Most Probable Number (MPN) Method

The MUG Test

Application for Soil, Sediment, and Sludge Samples

Analytical Quality Control (QC) Procedure

Determination of Fecal Streptococcus


Enterococci Portion of the Fecal Streptococcus Group

Membrane Filter (MF) Technique

Delayed MF Procedure

Most Probable Number (MPN) Method

Pour Plate Method

Procedures for Soils, Sediments, and Sludges

Analytical Quality Control (QC) Procedures



Enterotube II


API 20E System

Iron and Sulfur Bacteria


Iron Bacteria

Sulfur Bacteria

Enumeration, Enrichment, and Isolation of Iron and Sulfur Bacteria

Detection of Actinomycetes

General Introduction

Determination of Actinomycetes Density

Appendix A: Exponential Notation

Appendix B: International System of Units (Metric System)

Appendix C: Units and Conversion Factors

Appendix D: Biochemical Oxygen Demand (BOD)

Appendix E: Determination of Solids

Appendix F: Measurement of Ph

Appendix G: Bacteriophages

Subject Categories

BISAC Subject Codes/Headings:
TECHNOLOGY & ENGINEERING / Environmental / Water Supply