Capillary electrophoresis (CE) and microfluidic chip (MC) devices are relatively mature technologies, but this book demonstrates how they can be integrated into a single, revolutionary device that can provide on-site analysis of samples when laboratory services are unavailable. By introducing the combination of CE and MC technology, Microfluidic Chip-Capillary Electrophoresis Devices broadens the scope of chemical analysis, particularly in the biomedical, food, and environmental sciences.
The book gives an overview of the development of MC and CE technology as well as technology that now allows for the fabrication of MC-CE devices. It describes the operating principles that make integration possible and illustrates some achievements already made by the application of MC-CE devices in hospitals, clinics, food safety, and environmental research. The authors envision further applications for private and public use once the proof-of-concept stage has been passed and obstacles to increased commercialization are addressed.
A summary of the current methodology and application of MC-CE devices, including their merits, limitations, and potential, closes the book. It highlights the path to be taken for future development of MC-CE and anticipates new advances in technology that will increase their performance and use as powerful analytical tools. With enhanced power, their scope of applications will widen and yield new benefits to researchers and the general public.
Microfluidic Chip-Capillary Electrophoresis: Expanding the Scope of Application for On-Site Analysis of Difficult Samples. Instrumentation and Facilities I: Fabrication of Microfluidic Chip-Capillary Electrophoresis Device. Instrumentation and Facilities II: Manipulating Nanofluids for Desired Operations to Achieve Intended Application by MC-CE Device. Single-Channel Mixing: Extending Analytical Range to Handle Samples with High Analyte Variation for Profiling Urinary Organic and Inorganic Anions. Dual-Channel Mixing: Standard Addition by Dual-Channel Continuous Flow Mixing for Assay of Complex Nephrolithiasis Biomarkers in Urine. Sample Cleanup and Analyte Enrichment: Integrating MIP/SPE with ME-MC-CE Device to Enhance Sample Cleanup and Multidimensional Analyte Enrichment for Hourly Determination of Atmospheric Carbonyl Compounds. Enhancing Separation Efficiency: On-Chip Multidimensional Separation and Determination of Urinary Proteins by MC-CE Device. Improving Detection Selectivity: MC-CE Device Integrated with Dual Opposite Carbon-Fiber Microdisk Electrodes Detection for Determining Polyphenols in Wine. Enhancing Detection Versatility: MC-CE Device with Serial Dual-Electrode Detection for Determining Glutathione Disulfide and Glutathione in Pharmaceutical Supplement. Detecting Optically Inactive Analyte: MC-CE Device Integrated with QDs/LIF Detection for Determination of Acrylamide in Food. Quantum Dots-Enhanced Fluorescence Detection: Immobilized Quantum Dots for Laser-Induced Fluorescence Detection of Organophosphorus Pesticides in Vegetables. On-Site Assay by Portable UV Detector: MC-CE Device for Authenification of Chinese Medicine. On-Chip Binding Assay: MC-CE Device Integrated with Multisegment Circular-Ferrofluid-Driven Micromixing Injection for Assay of Free Bilirubin and Albumin Residual Binding Capacity for Bilirubin. Counting Organelles: Direct Counting of Mitochondrial Numbers. Indirect Assessment of Organelles in Cell Extract: Determination of M