Preface
List of Contributors
1. Nucleic Acid Extraction from Diverse Samples
Scott O. Rogers
2. DNA Extraction from Mummified Tissues
Yayoi Sato
3. Commercial DNA Extraction Kits
Stuart C. Clarke
4. Automated Nucleic Acid Extraction
Juergen Loeffler, Kathrin D. Schmidt, Holger Hebart, Hermann Einsele
5. Forensic DNA Samples: Collection and Handling
Mark Benecke
6. DNA Preservation
C. William Kilpatrick
7. RNA Storage
Martin Hofmann
8. Disposable Electrochemical DNA Biosensors
Kagan Kerman and Mehmet Ozsoz
9. Handheld Nucleic Acid Analyzer
James A. Higgins
10. DNA-Binding Fluorophores
Mikael Kubista, Jonas Karlsson, Martin Bengtsson, Neven Zoric, Gunar Westman
11. Fluorescence Resonance Energy Transfer (FRET)
Dino A. De Angelis
12. LightUp® Probes
Mikael Leijon
13. Invader® Assay
Stephen Day, Andrea Mast
14. Laser-Capture Microdissection
Falko Fend
15. Helper-Dependent Adenoviral Vectors
Philip Ng
16. Retroviral Vectors
Erin L. Weber, Paula M. Cannon
17. Liposomal Nonviral Delivery Vehicles
Nancy Smyth Templeton
18. Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing
Janin Germer, Ernst Wagner
19. Automated DNA Sequencing
Alexandre Izmailov
20. DNA Sequencing Methods
Scott O. Rogers
21. Differential Sequencing by Mass Spectrometry
Christian Jurinke, Christiane Honisch, Dirk van den Boom
22. Brief Guide to Conducting Biological Database Searches
John Gray
23. Accessing Genomic Databases
Robert J. Trumbly
24. Phylogenetics, Comparative Genomics, and Phylogenomics
Scott O. Rogers
25. DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences
Chunyu Liu
26. ESTAnnotator: A Tool for High-Throughput EST Annotation
Agnes Hotz-Wagenblatt and Thomas Hankeln
27. The Basics of Omics
Scott O. Rogers
28. Ribotyping
Tim J. Inglis
29. Forensic DNA Typing: Y Chromosome
Lluís Quintana-Murci
30. Forensic Identification: An Overview on Molecular Diagnostic Technology
Adrian Linacre, Yvonne E. Cruickshank
31. Denaturing Gradient Gel Electrophoresis (DGGE)
Luciana Cresta de Barros Dolinsky
32. Heteroduplex Analysis (HA)
Philip L. Beales
33. NEBcutter: A Program to Cleave DNA with Restriction Enzymes
Janos Posfai, R. J. Roberts, Tamas Vincze
34. Differential Display (DD) Analysis
Farid E. Ahmed
35. Protein Truncation Test (PTT)
Sadanand Gite, Mark Lim, Kenneth Rothschild
Index
Biography
Scott Orland Rogers is a professor of molecular biology and evolution at Bowling Green State University, Bowling Green, Ohio. He received his PhD in plant molecular biology from the University of Washington, Seattle. He was an assistant professor and associate professor at the State University of New York College of Environmental Science and Forestry before moving to BGSU. He has taught courses in biology, botany, cell physiology, molecular biology, molecular genetics, bioinformatics, and molecular evolution. Research in his lab includes studies of microbes and nucleic acids preserved in ice, life in extreme environments, group I introns, molecular microbial phylogenetics, microbial metagenomics/metatranscriptomics, ancient DNA, and plant development.
