1st Edition

Molecular Approaches for the Detection of Fungal Phytopathogens

304 Pages 43 B/W Illustrations
by CRC Press

304 Pages 43 B/W Illustrations
by CRC Press

This book discusses recent advancements and updates in the molecular diagnostics of fungal phytopathogens. The techniques covered in the seventeen chapters help in understanding the importance of early detection of fungal pathogens and their toxins in both post- and pre-harvest stages of crop production. Molecular Approaches for the Detection of Fungal Phytopathogens describes the fungal... Read more

Chapter 1: Fungal phytopathogens and importance of their early detection through conventional methods
K. Ajithkumar, A. S. Savitha, and M. Renuka  

Chapter 2: Fungal phytopathogens diagnostic: A historical perspective
Girish H. V. and Raghavendra M.P. 

Chapter 3: Methodology for the Isolation and Identification of Fungal Pathogens
Mahadevakumar S. , Josna Joy, M. Mahesh and Sridhar K. R. 

Chapter 4: Polymerase chain reaction and its variants as detection tool for fungal phytopathogens
Deepa Nagaraju and Sreenivasa Marikunte Yanjarappa

Chapter 5: Loop-mediated isothermal amplification (LAMP) for the detection and quantification of fungal phytopathogens
Wily Sic, Sandra V. Gomez, and Stephen B. Goodwin

Chapter 6: Oligonucleotide array approaches for the detection of plant pathogenic fungi
Vignesh Dhandapani, Teruo Sano and Charith Raj Adkar Purushothama

Chapter 7: An overview on application and advancements of lateral flow assay technique in effective detection of fungal phytopathogens
Bhavani PV

Chapter 8: DNA Barcoding for Identification of Fungal Pathogens                  
Mahadevakumar S., Ajithkumar K and Sridhar K. R. 

Chapter 9: Next Generation Sequencing, a new opportunity for discovering fungal phytopathogens
Sarvajith M. 

Chapter 10: In-situ hybridization for the detection of fungal phytopathogens               
Shruthi B., Adithi G. and Sreenivasa Marikunte Yanjarappa

Chapter 11: Serological assays for the detection of phytopathogenic fungi
Dhanush. S, Maheshwar P. K.

Chapter 12: Detection of fungal phytopathogens by nanotechnology method               
Manjunatha Dadayya, Sowmya Hirakannavar Veeranna and Thippeswamy Basaiah

Chapter 13: Aptamers: A Promising Tool for the Detection of Phytopathogenic Fungal Toxins
Shylaja R, Rakesh R and Joseph Kingston

Chapter 14: On-site diagnostic techniques for fungal pathogens
Vignesh Dhandapani, Madanraj Appiya Santharam, Suriya Tamilselvan-Nattar-Amutha, Charith Raj Adkar Purushothama

Chapter 15: Policies, Regulations and Quality Assurance Requirements for Sustainable Agriculture
S. Sundaramoorthy and V. Jaiganesh

Chapter 16: Policies, Regulations and Quality Assurance requirements for the agri-food industry
Balaji G, Hari Sankar SS, Subhaprada Nishtala, Anuja Bejoy and Sanu Jacob

Chapter 17: Further Directions in Fungal Phytopathogen Diagnostics
Sridhar K. R. and Mahadevakumar S. 

Biography

Deepa Nagaraju is Research Associate for Indian Council of Medical Research funded project on “Potential antifungals from PGPR bacteria against fumonisin” at the Department of Studies in Microbiology, University of Mysore, Mysuru, India. Her research interests include molecular mycology, myco-toxicology and its prevention. She earned her doctoral degree from University of Mysore in 2018 with her thesis entitled “Development of nested and multiplex PCR for the early detection of fumonisin producing Fusarium verticillioides associated with cereals”. She worked as a Project Assistant for three years on the DST-Fastrack funded project as well as a Senior Research Fellow for ICMR funding for three years.  She served as a faculty for Postgraduate students for four years at the Department of Studies in Microbiology, University of Mysore, Mysuru. She has authored 31 scientific papers, which include 5 book chapters and as well as a book.

Sreenivasa Marikunte Yanjarappa is currently working as a Professor in Microbiology and has more than 20 years of teaching experience at the Department of Studies in Microbiology, University of Mysore, Mysuru, India. His research interests include mycology, myco-toxicology, probiotics and postbiotics. In recognition of his research output, he was awarded Raman postdoctoral fellow to carry out his post-doctoral studies at Department of Plant Pathology, University of Arkansas, USA. In recognition of Indo – US Research collaboration, he was awarded International Faculty Development award by the Kennesaw State University, Kennesaw, USA. He was Visiting Research Scientist at the University of Ottawa, Canada under Shastri Indo-Canadian Institute, India. He has successfully completed 7 research projects in the field of microbiology funded by national and international agencies. He has an Indian patent and has published more than 100 scientific papers, book chapters and editorial articles with an H-index of 30, i10 index of 55 and more than 2200 citations. He is a member of the review editorial boards of several international journals. As an editor, he has also published two books. He has been invited as Keynote Speakers and Expert in various Plant pathology, microbiology and mycology as well as Life Sciences-based conferences and workshops.

Charith Raj Adkar Purushothama is an adjunct professor at the Department of Biochemistry and Functional Genomics, Université de Sherbrooke, Canada, and a biotechnology entrepreneur. He earned his master’s degree in microbiology from the University of Mysore, India, and his doctorate from the School of Plant Biology and Crop Production, University of Milano, Italy. He did his post-doctoral training in the laboratory of Prof. Teruo Sano at Hirosaki University, Japan, and in the laboratory of Prof. Jean-Pierre Perreault, at Université de Sherbrooke, Canada. During his post-doctoral training, he worked on plant-pathogen interactions and management strategies. He received several research scholarships and grants at national and international level. He has published over 60 original research articles, two review articles, and several book chapters, and presented in more than 30 international scientific conferences. In 2017, one of his research publications on viroid received the “Paper of Excellence” award from The Phytopathological Society of Japan.