1st Edition

Molecular, Cellular, and Tissue Engineering

    1892 Pages 461 B/W Illustrations
    by CRC Press

    Known as the bible of biomedical engineering, The Biomedical Engineering Handbook, Fourth Edition, sets the standard against which all other references of this nature are measured. As such, it has served as a major resource for both skilled professionals and novices to biomedical engineering.

    Molecular, Cellular, and Tissue Engineering, the fourth volume of the handbook, presents material from respected scientists with diverse backgrounds in molecular biology, transport phenomena, physiological modeling, tissue engineering, stem cells, drug delivery systems, artificial organs, and personalized medicine.

    More than three dozen specific topics are examined, including DNA vaccines, biomimetic systems, cardiovascular dynamics, biomaterial scaffolds, cell mechanobiology, synthetic biomaterials, pluripotent stem cells, hematopoietic stem cells, mesenchymal stem cells, nanobiomaterials for tissue engineering, biomedical imaging of engineered tissues, gene therapy, noninvasive targeted protein and peptide drug delivery, cardiac valve prostheses, blood substitutes, artificial skin, molecular diagnostics in personalized medicine, and bioethics.


    Historical Perspective and Basics of Molecular Biology; Nathan R. Domagalski and Michael Domach

    Biomolecular Interactions; Gordon Rule

    Recent Advances in DNA Separations: Plasmid Purification, Rapid Electrophoresis, and Affinity-Based Recovery; James W. Schneider and Jeffrey M. Savard

    Systems and Technology Involving Bacteria; Nicole Bleckwenn and William Bentley

    Expression in Mammalian Cells; Tina Sauerwald and Michael Betenbaugh

    DNA Vaccines Production and Engineering; Michael M. Domach, Jonathan W. Meade, and Mohammad A. Ataai


    Biomimetic Systems: Concepts, Design, and Emulation; Robert J. Fisher

    Transport/Reaction Processes in Biology and Medicine; E. N. Lightfoot

    Microvascular Heat Transfer; James W. Baish

    Fluid Dynamics for Bio Systems: Fundamentals and Model Analysis; Robert A. Peattie and Robert J. Fisher

    Animal Surrogate Systems; Michael L. Shuler, Sarina G. Harris, Xinran Li, and Mandy B. Esch

    Arterial Wall Mass Transport: The Possible Role of Blood Phase Resistance in the Localization of Arterial Disease; John M. Tarbell and Yuchen Qiu

    Transport Phenomena and the Microenvironment; Robert J. Fisher and Robert A. Peattie

    Transport and Drug Delivery through the Blood–Brain Barrier and Cerebrospinal Fluid; Bingmei M. Fu

    Interstitial Transport in the Brain: Principles for Local Drug Delivery; W. Mark Saltzman

    Surfactant Transport and Fluid–Structure Interactions during Pulmonary Airway Reopening; David Martin, Anne-Marie Jacob, and Donald P. Gaver III


    Modeling Strategies and Cardiovascular Dynamics; Joseph L. Palladino, Gary M. Drzewiecki, and Abraham Noordergraaf

    Compartmental Models of Physiological Systems; Claudio Cobelli, Giovanni Sparacino, Maria Pia Saccomani, Gianna Maria Toffolo, and Andrea Caumo

    Cardiovascular Models and Control; Madhusudan Natarajan, Fernando Casas, and W.D. Timmons

    Respiratory Models and Control; Chi-Sang Poon

    Biomimetic Approaches to Physiological Control; James J. Abbas and Amit Abraham

    Methods and Tools for Identification of Physiologic Systems; Vasilis Z. Marmarelis

    Modeling Vascular Vibrations: Autoregulation and Vascular Sounds; Gary M. Drzewiecki, John K.-J. Li, and Abraham Noordergraaf

    External Control of Movements; Dejan B. Popovic´ and Mirjana B. Popovic

    The Fast Eye Movement Control System; John Denis Enderle

    A Comparative Approach to Analysis and Modeling of Cardiovascular Function; John K.-J. Li, Ying Zhu, and Abraham Noordergraaf

    A Biomedical and Biophysical Approach to the Science in Cardiopulmonary Resuscitation; Gerrit J. Noordergraaf, Igor W. F. Paulussen, Alyssa Venema, Gert Jan Scheffer, and Abraham Noordergraaf

    Kinematic Modeling of Left Ventricular Diastolic Function; Leonid Shmuylovich, Charles S. Chung, and Sándor J. Kovács


    Engineering the Pluripotent Stem Cell Niche for Directed Mesoderm Differentiation; Céline L. Bauwens, Kelly A. Purpura, and Peter W. Zandstra

    Cell Mechanobiology in Regenerative Medicine: Lessons from Cancer; Badriprasad Ananthanarayanan and Sanjay Kumar

    Systems-Engineering Principles in Signal Transduction and Cell-Fate Choice; Karin J. Jensen, Anjun K. Bose, and Kevin A. Janes

    Biomaterial Scaffolds for Human Embryonic Stem Cell Culture and Differentiation; Stephanie Willerth and David Schaffer

    Stem Cells and Regenerative Medicine in the Nervous System; Shelly Sakiyama-Elbert

    Stem Cells and Regenerative Medicine for Treating Damaged Myocardium; Rohini Gupta, Kunal Mehtani, Kimberly R. Kam, and Kevin E. Healy

    Stem Cells and Hematopoiesis; Krista M. Fridley and Krishnendu Roy

    Synthetic Biomaterials and Stem Cells for Connective Tissue Engineering; Ameya Phadke and Shyni Varghese

    Derivation and Expansion of Human Pluripotent Stem Cells; Sean P. Palecek

    Bioreactors for Stem Cell Expansion and Differentiation; Carlos A. V. Rodrigues, Tiago G. Fernandes, Maria Margarida Diogo, Cláudia Lobato da Silva, and Joaquim M. S. Cabral


    Strategic Directions; Peter C. Johnson

    Silks; Monica A. Serban and David L. Kaplan

    Calcium Phosphates; Kemal Sariibrahimoglu, Joop G.C. Wolke, Sander C.G. Leeuwenburgh, and John A. Jansen

    Engineered Protein Biomaterials; Andreina Parisi-Amon and Sarah C. Heilshorn

    Synthetic Biomaterials; Joshua S. Katz and Jason A. Burdick

    Growth Factors and Morphogens: Signals for Tissue Engineering; A. Hari Reddi

    Signal Expression in Engineered Tissues; Martha O. Wang and John P. Fisher

    Pluripotent Stem Cells; Todd C. McDevitt and Melissa A. Kinney

    Hematopoietic Stem Cells; Ian M. Kaplan, Sebastien Morisot, and Curt I. Civin

    Mesenchymal Stem Cells; Pamela C. Yelick and Weibo Zhang

    Nanobiomaterials for Tissue Engineering; Pramod K. Avti, Sunny C. Patel, Pushpinder Uppal, Grace O’Malley, Joseph Garlow, and Balaji Sitharaman

    Biomimetic Approaches in Tissue Engineering; Indong Jun, Min Sup Kim, Ji-Hye Lee, Young Min Shin, and Heungsoo Shin

    Molecular Biology Techniques; X.G. Chen, Y.L. Fang, and W.T. Godbey

    Biomaterial Mechanics; Kimberly M. Stroka, Leann L. Norman, and Helim Aranda-Espinoza

    Mechanical Conditioning; Elaine L. Lee and Horst A. von Recum

    Micropatterned Biomaterials for Cell and Tissue Engineering; Murugan Ramalingam and Ali Khademhosseini

    Drug Delivery; Prinda Wanakule and Krishnendu Roy

    Gene Therapy; C. Holladay, M. Kulkarni, W. Minor, and Abhay Pandit

    Nanotechnology-Based Cell Engineering Strategies for Tissue Engineering and Regenerative Medicine Applications; Joaquim Miguel Oliveira, João Filipe Mano, and Rui Luís Reis

    Cell Encapsulation; Stephanie J. Bryant

    Coculture Systems for Mesenchymal Stem Cells; Song P. Seto and Johnna S. Temenoff

    Tissue Engineering Bioreactors; Sarindr Bhumiratana, Elisa Cimetta, Nina Tandon, Warren Grayson, Milica Radisic, and Gordana Vunjak-Novakovic

    Shear Forces; Jose F. Alvarez-Barreto, Samuel B. VanGordon, Brandon W. Engebretson, and Vasillios I. Sikavitsas

    Vascularization of Engineered Tissues; Monica L. Moya and Eric M. Brey

    Biomedical Imaging of Engineered Tissues; Nicholas E. Simpson and Athanassios Sambanis

    Multiscale Modeling of In Vitro Tissue Cultivation; Kyriacos Zygourakis

    Bone Engineering; Lucas A. Kinard, Antonios G. Mikos, and F. Kurtis Kasper

    Dental and Craniofacial Bioengineering; Hemin Nie and Jeremy J. Mao

    Tendon and Ligament Engineering; Nicholas Sears, Tyler Touchet, Hugh Benhardt, and Elizabeth Cosgriff-Hernández

    Cartilage Tissue Engineering; Emily E. Coates and John P. Fisher

    TMJ Engineering; Michael S. Detamore

    Interface Tissue Engineering; Helen H. Lu, Nora Khanarian, Kristen Moffat, and Siddarth Subramony

    The Bioengineering of Dental Tissues; Rena N. D’Souza, Katherine R. Regan, Kerstin M. Galler, and Songtao Shi

    Tissue Engineering of the Urogenital System; In Kap Ko, Anthony Atala, and James J. Yoo

    Vascular Tissue Engineering; Laura J. Suggs

    Neural Engineering; Yen-Chih Lin and Kacey G. Marra

    Tumor Engineering: Applications for Cancer Biology and Drug Development; Joseph A. Ludwig and Emily Burdett


    Artificial Heart and Circulatory Assist Devices; Gerson Rosenberg

    Cardiac Valve Prostheses; Arjunon, Neelakantan Saikrishnan, and Ajit P. Yoganathan

    Artificial Lungs; Keith E. Cook, Timothy M. Maul, and William J. Federspiel

    Blood Substitutes; Amy G. Tsai, Pedro Cabrales, and Marcos Intaglietta

    Liver Support Systems; Matthew S. Chang and Robert S. Brown

    Peritoneal Dialysis Equipment; Carlo Crepaldi, Francesca Katiana Martino, Maria Pia Rodighiero, and Claudio Ronco

    Artificial Skin and Dermal Equivalents; Dennis P. Orgill, Raul Cortes, and Ioannis V. Yannas


    Physiological Barriers to Drug Transport; Fan Yuan

    Nucleic Acid Aptamers in Drug Delivery; Mark R. Battig, Jing Zhou, and Yong Wang

    Dendrimers for Drug Delivery; Giridhar Thiagarajan and Hamidreza Ghandehari

    Noninvasive Targeted Protein and Peptide Drug Delivery; Pradeep K. Karla, Durga K. Paturi, Nanda K. Mandava, Animikh Ray, Sulabh Patel, Ranjana Mitra, and Ashim K. Mitra

    Environment-Responsive Hydrogels for Drug Delivery; Byung Kook Lee, Jong-Ryoul Kim, Kinam Park, and Yong Woo Cho

    Biodegradable PLGA Scaffolds for Growth Factor Delivery; Yusef Khan and Cato Laurencin


    Physiogenomic Contours: The Application of Systems Biology for Engineering Personalized Health Care; Andreas Windemuth, Richard L. Seip, and Gualberto Ruaño

    The Evolution of Massively Parallel Sequencing Technologies: Facilitating Advances in Personalized Medicine; Ian Toma, Georges St. Laurent, III, Samuel Darko, and Timothy A. McCaffrey

    Computational Methods and Molecular Diagnostics in Personalized Medicine; Roland Valdes, Jr. and Mark W. Linder

    Need for Point-of-Care Testing Devices for Cardiac Troponin in Patients with Acute Coronary Syndromes; Alan H.B. Wu and Amy E. Herr


    An Introduction to Bioethics and Ethical Theory for Biomedical Engineers; D. John Doyle


    Joseph D. Bronzino is the founder and president of the Biomedical Engineering Alliance and Consortium (BEACON) in Hartford, Connecticut. He earned a PhD in electrical engineering from Worcester Polytechnic Institute in Massachusetts. Dr. Bronzino has received the Millennium Award from IEEE/EMBS and the Goddard Award from Worcester Polytechnic Institute for Professional Achievement. He is the author of more than 200 articles and 11 books.

    Donald R. Peterson is a professor of engineering and dean of the College of Science, Technology, Engineering, Mathematics, and Nursing at Texas A&M University–Texarkana. He earned a PhD in biomedical engineering from Worcester Polytechnic Institute in Massachusetts. Dr. Peterson’s recent research focuses on measuring and modeling human, organ, and/or cell performance, including exposures to various physical stimuli and the subsequent biological responses. Dr. Peterson has published more than 50 journal articles and 12 reference books.