Molecular Regulation of Arousal States: 1st Edition (Paperback) book cover

Molecular Regulation of Arousal States

1st Edition

By Ralph Lydic

CRC Press

256 pages | 3 B/W Illus.

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Paperback: 9780849333613
pub: 1997-10-07
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Description

Arousal states are processes that include waking, deep sleep, and the dreaming phase of sleep (REM). Molecular Regulation of Arousal States explores the cellular and molecular mechanisms by which sleep and wakefulness are regulated and seeks explanations for the generation of arousal states. It presents step-by-step research protocols that allow investigators to apply the techniques described to a wide range of physiological and behavioral research problems, such as sleep neurobiology and state-dependent disruption of cardiopulmonary control. For the first time, a single source integrates cellular and molecular research techniques with studies of arousal, opening the door to exciting new research methodologies.

Table of Contents

Application of In Situ Hybridization to the Study of Rhythmic Neural Systems, H.E. Albers, R.T. Zoeller, and K.L. Huhman

Estimation of the mRNAs Encoding the Cholinergic Muscarinic Receptor and Acetylcholine Vesicular Transport Proteins Involved in Central Cardiovascular Regulation, J.J. Buccafusco, L.C. Zhang, and M.A. Prendergast

Voltammetric Detection of Nitric Oxide (NO) in the Rat Brain: Release Throughout the Sleep-Wake Cycle, S. Burlet and R. Cespuglio

Sleep Regulatory Substances: Change in mRNA Expression Linked to Sleep, Z. Chen and J.M. Krueger

Immediate Early Genes as a Tool to Understand the Regulation of the Sleep-Waking Cycle: Immunocytochemistry, In Situ Hybridization, and Antisense Approaches, C. Cirelli, M. Pompeiano, and G. Tononi

Methods for the Measurement of Adenylyl Cyclase Activity, C.W. Emala

Methods Used to Assess Specific Messenger RNA Expression During Sleep, M.A. Greco, L. Ramanathan, R. Basheer, and P.J. Shiromani

Competition Binding Assays for Determining the Affinity and Number of Muscarinic Receptor Subtypes in Tissue Homogenates, A.U. Höglund and H.A. Baghdoyan

Isolation and Identification of Specific Transcripts by Subtractive Hybridization, T.S. Kilduff, L. de Lecea, H. Usui, and J.G. Sutcliffe

Use of In Situ Hybridization Histochemistry to Study Muscarinic Receptor mRNA Expression in Brains of Sleep-Deprived Rats, C. Kushida and D.M. Simmons

Transcriptional Regulation of Putative Sleep Promoting Compounds, M. Mackiewicz, S.C. Veasey, and A.I. Pack

Chemical Mutagenesis and Screening for Mouse Mutations with an Altered Rest-Activity Pattern, P.M. Nolan, T.A. Houpt, and M. Bucan

Reverse Transcription mRNA Differential Display: A Systematic Molecular Approach to Identify Changes in Gene Expression Across the Sleep-Waking Cycle, M. Pompeiano, C. Cirelli, and G. Tononi

In Situ Hybridization of Messenger RNA in Sleep Research, T. Porkka-Heiskanen, J. Toppila, and D. Stenberg

New Directions in the Analysis of Brain Substances Related to Sleep and Wakefulness, G. Siuzdak and S. Henriksen

Sleep and Circadian Rest-Activity Rhythms in Prion Protein Knockout Mice, I.M. Tobler, M. Fischer, and J. Manson

Measurement of Nitric Oxide in the Brain Using the Hemoglobin Trapping Technique Coupled with In Vivo Microdialysis, J.A. Williams, S.R. Vincent, and P.B. Reiner

Mapping Regional Cerebral Protein Synthesis During Sleep, R.K. Zoltoski

Index

Subject Categories

BISAC Subject Codes/Headings:
MED071000
MEDICAL / Pharmacology
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology
SCI089000
SCIENCE / Life Sciences / Neuroscience