Molecular and Cellular Methods in Developmental Toxicology: 1st Edition (Paperback) book cover

Molecular and Cellular Methods in Developmental Toxicology

1st Edition

By George P. Daston

CRC Press

304 pages

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Paperback: 9780849333422
pub: 1996-09-26
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Description

This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.

Table of Contents

Preface

Molecular Techniques

Molecular Techniques for Developmental Toxicology: An Introduction, G.P. Daston

Visualization of Gene Expression Patterns by In Situ Hybridization, U. Albrecht, G. Eichele, J.A. Helms, and H.-C. Lu

Utilizing Transgenic Mice to Study Gene Regulation and Function, M.R. Blackburn and R.E. Kellems

The Use of Antisense Oligonucleotide Technology and Mouse Whole-Embryo Culture to Study Gene Function During Organogenesis, L.F. Potts and T.W. Sadler

Nucleic Acid Amplification Technologies, R.H. Finnell, S.J. Vacha, and S.A. Mackler

Protein Identification

Fundamentals of Protein Identification: Biochemical and Immunological Analysis of Developmentally Altered Protein Expression, G.B. Grunwald

Immunohistochemistry, P.E. Mirkes and S. Little

Cellular Techniques

Cellular Techniques for Teratological Cell Death, T.B. Knudsen

Applications of Flow Cytometry in Developmental and Reproductive Toxicology, K.H. Elstein and R.M. Zucker

Modern Techniques for Cell Labeling in Avian and Murine Embryos, D.K. Darnell and G.C. Schoenwolf

About the Series

Methods in Life Sciences - Toxicology Section

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Subject Categories

BISAC Subject Codes/Headings:
MED096000
MEDICAL / Toxicology
SCI049000
SCIENCE / Life Sciences / Biology / Molecular Biology
SCI072000
SCIENCE / Life Sciences / Biology / Developmental Biology