1st Edition

Molecular and Cellular Methods in Developmental Toxicology

By George P. Daston Copyright 1997
    304 Pages
    by CRC Press

    304 Pages
    by CRC Press

    This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.

    Molecular Techniques
    Molecular Techniques for Developmental Toxicology: An Introduction, G.P. Daston
    Visualization of Gene Expression Patterns by In Situ Hybridization, U. Albrecht, G. Eichele, J.A. Helms, and H.-C. Lu
    Utilizing Transgenic Mice to Study Gene Regulation and Function, M.R. Blackburn and R.E. Kellems
    The Use of Antisense Oligonucleotide Technology and Mouse Whole-Embryo Culture to Study Gene Function During Organogenesis, L.F. Potts and T.W. Sadler
    Nucleic Acid Amplification Technologies, R.H. Finnell, S.J. Vacha, and S.A. Mackler
    Protein Identification
    Fundamentals of Protein Identification: Biochemical and Immunological Analysis of Developmentally Altered Protein Expression, G.B. Grunwald
    Immunohistochemistry, P.E. Mirkes and S. Little
    Cellular Techniques
    Cellular Techniques for Teratological Cell Death, T.B. Knudsen
    Applications of Flow Cytometry in Developmental and Reproductive Toxicology, K.H. Elstein and R.M. Zucker
    Modern Techniques for Cell Labeling in Avian and Murine Embryos, D.K. Darnell and G.C. Schoenwolf


    Daston, George P.