The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.
1. Techniques for the production and characterisation of monoclonal hybridoma antibodies 2. Analysis of Monoclonal antibodies to human growth hormone and related protein 3. Antibodies to Alpha-fetoprotein and Carcinoembryonic Antigen produced by Somatic Cell Fusion 4. Monoclonal Antibodies specific for Cardiac Myosin- In Vivo and In Vitro Diagnostic Tools in Myocardial Infarction 5. The use of monoclonal Antibodies to investigate antigenic Drift in Influenza Virus 6. Monoclonal Antibodies to herpes simplex viruses 1 and 2 7. Hybridoma in Immunopharmacology 8. Hybridoma Antibodies Specific for Human Tumour Antigens 9. Murine Macrophage Differentiation Antigens Defined by Monoclonal Antibodies 10. Application of Monoclonal Antibodies to the Study of Human Lymphocyte Surface Antigens 11. Monoclonal Antibodies to the Major Histocompatibility Antigens