222 pages | 68 B/W Illus.
A Unified Microscopic Approach to Analyzing Complex Processes in Molecular Motors
Motor Proteins and Molecular Motors explores the mechanisms of cellular functioning associated with several specific enzymatic molecules called motor proteins. Motor proteins, also known as molecular motors, play important roles in living systems by supporting cellular transport and force generation via the transformation of chemical energy into mechanical work.
The book presents established results, theoretical methods, and experimental observations related to biological molecular motors. It uses fundamental physical-chemical concepts and methods to develop a systematic theoretical framework for understanding motor protein dynamics. The author introduces the main ideas using simple arguments that avoid heavy mathematical derivations in favor of more intuitive physical understanding. Although the book assumes some rudimentary knowledge of cell biology, calculus, and basic ideas from chemistry and physics, it gives explanations and derivations for most results.
Accessible to students and researchers in a wide range of scientific fields, this book provides a unified molecular picture for analyzing motor proteins. It connects major experimental facts on molecular motors to principal theoretical concepts consistent with the fundamental laws of chemistry and physics.
"Life is a motion!" (p. xvii) opens this volume that endeavors to provide a physical-chemical framework for understanding the working principles of motor proteins…The first part of the book introduces the physical foundations, starting with diffusion and then discusses a number of techniques, including stopped flow, force spectroscopy, and super-resolution microscopy. The second part covers fundamental physical and chemical concepts, starting with equilibrium approaches before moving to nonequilibrium approaches and enzyme kinetics. Two pleasing touches are biographical details of the scientists who established the founding principles, and the inclusion of questions important to the motors field that are used to frame the discussion of the theoretical concepts.
In the third and defining section of the volume, the author presents two theoretical approaches for modeling molecular motors: Continuum Ratchets and Discrete-State Stochastic models. Having published a number of papers using both approaches, Kolomeisky is the ideal person to present the foundations as well as the strengths and weaknesses of each approach…Overall, the volume is clearly written and will be of value to graduate students and researchers across multiple disciplines related to mechanobiology and nanotechnology. The treatment of modeling approaches is unique and the strongest aspect of the book, but the coverage of fundamental experimental and theoretical concepts underlying motor protein research will also be of value for researchers spanning cell biology, biophysics, and applied mathematics. Most importantly, this volume focuses most on the physical-chemical fundamentals of motor proteins, which will remain foundational as the field moves forward."
—William O. Hancock, Pennsylvania State University, in THE QUARTERLY REVIEW OF BIOLOGY, Volume 93, June 2018
"As a chemical physicist dabbling in molecular biophysics, I am particularly intimidated by the subject of molecular machines. Reading much of what is written on this subject is like trying to understand how the internal combustion engine works by looking at the blueprints of a modern car engine, with the added difficulty of the blueprints often being inaccurate or incomplete.
In contrast, Kolomeisky goes straight into the heart of the matter and explains why and how fundamental principles of physics and chemistry allow those marvelous molecular assemblies to generate directional motion and to perform every mechanical task in the living organism or cell. The author further describes how this molecular motion is studied experimentally, develops quantitative models that explain experimental observations, and provides a succinct, self-contained, and lucid introduction to the fundamentals of thermodynamics, statistical mechanics, and chemical kinetics relevant to molecular motors as well as to many other biochemical processes. The historical bits dispersed throughout the text make it especially refreshing to read and would be appreciated by any reader who, like myself, is amazed by the progress science has made in understanding the complexity of life."
—Dmitrii Makarov, Department of Chemistry, The University of Texas at Austin
MOTOR PROTEINS IN BIOLOGICAL SYSTEMS
DISCUSSION OF THEORETICAL MODELS FOR MOLECULAR MOTORS
MOTOR PROTEINS AS NANOSCALE MACHINES
Basic Properties of Motor Proteins
HISTORY OF MOTOR PROTEINS
CLASSIFICATION OF BIOLOGICAL MOLECULAR MOTORS
STRUCTURES OF MOTOR PROTEINS
BIOLOGICAL FUNCTIONS OF MOLECULAR MOTORS
Experimental Studies of Motor Proteins
BULK CHEMICAL-KINETIC MEASUREMENTS
SINGLE-MOLECULE FORCE SPECTROSCOPY
FLUORESCENT LABELING AND SUPER-RESOLUTION TECHNIQUES
MAJOR EXPERIMENTAL OBSERVATIONS
Fundamental Physical Concepts: Equilibrium Approaches
BASIC EQUILIBRIUM THERMODYNAMICS
BASIC STATISTICAL MECHANICS
APPLICATION FOR MOTOR PROTEINS
Fundamental Physical Concepts: Non-Equilibrium Approaches
MACROSCOPIC CHEMICAL KINETICS
Motor Proteins as Enzymes
Theory for Motor Proteins: Continuum Ratchets
CONTINUUM RATCHET POTENTIALS
Theory for Motor Proteins: Discrete-State Stochastic Models
DISCRETE-STATE STOCHASTIC APPROACH
Collective Properties of Motor Proteins
COOPERATIVITY AND INTERACTIONS IN MOTOR PROTEINS DYNAMICS
Artificial Molecular Motors and Rotors
BIOLOGICAL ARTIFICIAL MOLECULAR MOTORS
NON-BIOLOGICAL ARTIFICIAL MOLECULAR MOTORS
ARTIFICIAL MOLECULAR ROTORS
Future Directions in Studies of Motor Proteins and Molecular Motors
WHAT WE UNDERSTAND NOW ABOUT MOTOR PROTEINS AND MOLECULAR MOTORS
OPEN QUESTIONS AND PROBLEMS
LOOKING INTO THE FUTURE