Over the past decade, a myriad of techniques have shown that solid-state nuclear magnetic resonance (NMR) can be used in a broad spectrum of applications with exceptionally impressive results. Solid-state NMR results can yield high-resolution details on the structure and function of many important biological solids, including viruses, fibril-forming molecules, and molecules embedded in the cell membrane.
Filling a void in the current literature, NMR Spectroscopy of Biological Solids examines all the recent developments, implementation, and interpretation of solid-state NMR experiments and the advantages of applying them to biological systems. The book emphasizes how these techniques can be used to realize the structure of non-crystalline systems of any size. It explains how these isotropic and anisotropic couplings interactions are used to determine atomic-level structures of biological molecules in a non-soluble state and extrapolate the three-dimensional structure of membrane proteins using magic-angle spinning (MAS). The book also focuses on the use of multidimensional solid-state NMR methods in the study of aligned systems to provide basic information about the mechanisms of action of a variety of biologically active molecules.
Addressing principles, methods, and applications, this book provides a critical selection of solid-state NMR methods for solving a wide range of practical problems that arise in both academic and industrial research of biomolecules in the solid state. NMR Spectroscopy of Biological Solids is a forward-thinking resource for students and researchers in analytical chemistry, bioengineering, material sciences, and structural genomics.
Table of Contents
Introduction to Solid-State NMR Spectroscopy. Analysis of Pulse Sequences Using Computer Simulations. Measurement of NMR Parameters from Aligned Biological Solids. Variation of Chemical Shift Tensors in Proteins. Assignment of Resonances from Uniformly Labeled Proteins. Methods to Measure Interatomic Distances. Multidimensional Experiments to Study Solids Under Magic Angle Spinning. Fluorine-19 NMR to Enhance the Sensitivity. Proton-Detected Solid-State NMR Experiments. Structure, Dynamics, and Mechanism of Membrane-Disruptive Peptides. Topology of Membrane-Associated Proteins. Spin Interaction Tensors and Protein Dynamics in Model Membranes. Structure of Crystalline Proteins. Structure and Assembly of Amyloid Fibrils. Structure of Ligands Bound to Receptor Proteins. Structure and Mechanism of Fusion Peptides. Investigation of Metal-Bounds Proteins.