1st Edition

New Techniques for Studying Biomembranes

Edited By Qiu-Xing Jiang Copyright 2020
    180 Pages 22 Color & 22 B/W Illustrations
    by CRC Press

    180 Pages 22 Color & 22 B/W Illustrations
    by CRC Press

    180 Pages 22 Color & 22 B/W Illustrations
    by CRC Press

    New Techniques for Studying Biomembranes describes some of the latest methods used to investigate the dynamic distribution of specific lipids in membranes and their effects on other membrane components. The contributors present important discoveries with respect to lipid analysis and lipid interactions with membrane proteins. Various methods, which have been used to study lipid bilayer structure and lipid organization in membranes, include both in vitro and in vivo membrane systems, and study membrane proteins in various membrane systems.

    Key Features:

    • Reviews both in vivo and in vitro analytical technologies and methods for studying membrane structure and function
    • Explores how lipid bilayers and membrane proteins interact
    • Includes contributions from an international team of researchers actively studying membrane structure and function
    • Identifies various diseases whose causes are related to membrane proteins

    Related Titles:

    Christopher R. Jacobs, Hayden Huang, and Ronald Y. Kwon. Introduction to Cell Mechanics and Mechanobiology (ISBN 978-0-8153-4425-4)

    Wendell Lim and Bruce Mayer. Cell Signaling: Principles and Mechanisms (ISBN 978-0-8153-4244-1)

    Stephen Rothman. Proteins Crossing Membranes: A Scientist’s Memoir (978-0-3670-7449-4)

    Introduction. Lipid compositions of cell membranes and quantification. Phase behavior and lipid organization in membranes. Atomic Force Microscopy of biomembranes. Secondary Ion Mass spectrometry of membrane lipids. Super-resolution imaging of lipid membranes. Single molecule imaging of lipids and proteins in membranes. Membrane interferometry for studying lipids and proteins. Solid state NMR to study of membrane proteins in membranes. X-ray diffractions from stacked membranes. Neutron diffractions from membrane proteins in membranes. cryoEM of Membrane Proteins in membrane. Single molecular fluorimetry for membrane proteins. FRET measurement of ion channel in membrane. X-ray crystallography of membrane proteins.


    Qiu-Xing Jiang is faculty in the Department of Microbiology and Cell Science and and Electron Microscopy Faculty Director at the University of Florida's Interdisciplinary Center for Biotechnology Research. His laboratory is working in two areas. One is the structural and functional studies of membrane proteins and intracellular signaling complexes. The second general direction is the development of new techniques for both single particle cryoEM and for studying eukaryotic membrane proteins in lipid environments.

    "Each chapter begins with an introduction that carefully covers the fundamental
    operating principles of each method illuminated by details of its historical
    development. This extends to the mathematical functions encountered in the
    chapters on scanning angle interference microscopy and the analysis of protein
    function in reconstituted membrane systems. Each chapter appears to be well
    illustrated and thoroughly referenced. The end result is a concise and
    straightforward introduction to the topics selected for each chapter." - Peter J. Kennelly, PhD(Virginia Tech)