1st Edition
PCR/RT- PCR in situ Light and Electron Microscopy
444 Pages
21 Color & 237 B/W Illustrations
by
CRC Press
432 Pages
by
CRC Press
Also available as eBook on:
Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene... Read more
GENERAL PRINCIPLES
PREPARATION OF SAMPLES
PRETREATMENTS
REVERSE TRANSCRIPTION (RT)
POLYMERASE CHAIN REACTION (PCR)
HYBRIDIZATION
REVELATION
ELECTRON MICROSCOPY
CONTROLS AND PROBLEMS
TYPICAL PROTOCOLS
EXAMPLES OF OBSERVATIONS
APPENDICES
A-Equipment
B-Reagents
GLOSSARY
INDEX
Short TOC
General Principles. Preparation of Samples. Pretreatments. Reverse Transcription (RT). Polymerase Chain Reaction (PCR). Hybridization. Revelation. Electron Microscopy. Controls and Problems. Typical Protocols. Examples of Observations. Appendices. Glossary. Index
PREPARATION OF SAMPLES
PRETREATMENTS
REVERSE TRANSCRIPTION (RT)
POLYMERASE CHAIN REACTION (PCR)
HYBRIDIZATION
REVELATION
ELECTRON MICROSCOPY
CONTROLS AND PROBLEMS
TYPICAL PROTOCOLS
EXAMPLES OF OBSERVATIONS
APPENDICES
A-Equipment
B-Reagents
GLOSSARY
INDEX
Short TOC
General Principles. Preparation of Samples. Pretreatments. Reverse Transcription (RT). Polymerase Chain Reaction (PCR). Hybridization. Revelation. Electron Microscopy. Controls and Problems. Typical Protocols. Examples of Observations. Appendices. Glossary. Index
Biography
Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.
