2nd Edition

Phage Display In Biotechnology and Drug Discovery

Edited By Sachdev S. Sidhu, Clarence Ronald Geyer Copyright 2015
    by CRC Press

    584 Pages 150 B/W Illustrations
    by CRC Press

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    Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies.

    The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery.

    Key Features:

    • Describes the basic principles of phage display and the methods and systems that have been developed for key applications
    • Outlines applications for peptide phage display in the development of vaccines and high- throughput screens as well as the exploration of protein–protein interactions and enzyme substrate characteristics
    • Details the use of phage display in the engineering of protein stability, the identification and mapping of protein–protein interactions, and the development of catalysts
    • Provides broad coverage of the impact of phage display technology on the development of protein therapeutics
    • Presents expert opinions on future challenges in the field

    Filamentous Bacteriophage Structure and Biology
    Diane J. Rodi, Suneeta Mandava, and Lee Makowski
    Vectors and Modes of Display
    Valery A. Petrenko and George P. Smith
    Methods for the Construction of Phage-Displayed Libraries
    Frederic A. Fellouse and Gabor Pal
    Selection and Screening Strategies
    Mark S. Dennis
    Leveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell Surface
    Jelena Tomic, Megan McLaughlin, Traver Hart, Sachdev Sidhu, and Jason Moffat
    Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines
    Nienke E. van Houten and Jamie K. Scott
    Exploring Protein-Protein Interactions Using Peptide Libraries Displayed on Phage
    Kurt Deshayes
    Substrate Phage Display
    Shuichi Ohkubo
    Mapping Intracellular Protein Networks
    Malgorzata E. Kokoszka, Zhaozhong Han, Ece Karatan, and Brian K. Kay
    High-Throughput and High-Content Screening Using Peptides
    Robert O. Carlson, Robin Hyde-Deruyscher, and Paul T. Hamilton
    Engineering Protein Folding and Stability
    Mihriban Tuna and Derek N. Woolfson
    Identification of Natural Protein-Protein Interactions with cDNA Libraries
    Reto Crameri, Claudio Rhyner, Michael Weichel, Sabine Fluckiger, and Zoltan Konthur
    Mapping Protein Functional Epitopes
    Sara K. Avrantinis and Gregory A. Weiss
    Selections for Enzymatic Catalysts
    Julian Bertschinger, Christian Heinis, and Dario Neri
    Antibody Humanization and Affinity Maturation Using Phage Display
    Jonathan S. Marvin and Henry B. Lowman
    Antibody Libraries from Immunized Repertoires
    Jody D. Berry and Mikhail Popkov
    Naive Antibody Libraries from Natural Repertoires
    Claire L. Dobson, Ralph R. Minter, and Celia P. Hart-Shorrock
    Synthetic Antibody Libraries
    Frederic A. Fellouse and Sachdev S. Sidhu
    Engineering Antibody Fragments for Intracellular Applications
    Jianghai Liu and C. Ronald Geyer


    Edited by

    Sachdev S. Sidhu, PhD, professor, Department of Molecular Genetics, University of Toronto, Ontario, Canada

    C. Ronald Geyer, PhD, professor, Department of Pathology, University of Saskatchewan, Canada

    “… The book covers broadly all aspects of phage-display technology and will be highly appreciated by those working with this technique and interested in applications. … The book is a clear invitation to use the phage-display technique both in biotechnology and drug discovery; … this is a very comprehensive book demonstrating the versatility and the value of phage display in many applications. It is clearly written giving an interesting historical background to many presently used variations on the phage-display technique.”
    —Wim Quax, Rijksuniversiteit Groningen, The Netherlands, in ChemBioChem, Vol. 7, No. 699 - 703