2nd Edition

Phage Display In Biotechnology and Drug Discovery





ISBN 9781138894679
Published November 22, 2017 by CRC Press

USD $86.95

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Book Description

Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies.

The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery.

Key Features:

  • Describes the basic principles of phage display and the methods and systems that have been developed for key applications
  • Outlines applications for peptide phage display in the development of vaccines and high- throughput screens as well as the exploration of protein–protein interactions and enzyme substrate characteristics
  • Details the use of phage display in the engineering of protein stability, the identification and mapping of protein–protein interactions, and the development of catalysts
  • Provides broad coverage of the impact of phage display technology on the development of protein therapeutics
  • Presents expert opinions on future challenges in the field

Table of Contents

Filamentous Bacteriophage Structure and Biology
Diane J. Rodi, Suneeta Mandava, and Lee Makowski
Vectors and Modes of Display
Valery A. Petrenko and George P. Smith
Methods for the Construction of Phage-Displayed Libraries
Frederic A. Fellouse and Gabor Pal
Selection and Screening Strategies
Mark S. Dennis
Leveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell Surface
Jelena Tomic, Megan McLaughlin, Traver Hart, Sachdev Sidhu, and Jason Moffat
Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines
Nienke E. van Houten and Jamie K. Scott
Exploring Protein-Protein Interactions Using Peptide Libraries Displayed on Phage
Kurt Deshayes
Substrate Phage Display
Shuichi Ohkubo
Mapping Intracellular Protein Networks
Malgorzata E. Kokoszka, Zhaozhong Han, Ece Karatan, and Brian K. Kay
High-Throughput and High-Content Screening Using Peptides
Robert O. Carlson, Robin Hyde-Deruyscher, and Paul T. Hamilton
Engineering Protein Folding and Stability
Mihriban Tuna and Derek N. Woolfson
Identification of Natural Protein-Protein Interactions with cDNA Libraries
Reto Crameri, Claudio Rhyner, Michael Weichel, Sabine Fluckiger, and Zoltan Konthur
Mapping Protein Functional Epitopes
Sara K. Avrantinis and Gregory A. Weiss
Selections for Enzymatic Catalysts
Julian Bertschinger, Christian Heinis, and Dario Neri
Antibody Humanization and Affinity Maturation Using Phage Display
Jonathan S. Marvin and Henry B. Lowman
Antibody Libraries from Immunized Repertoires
Jody D. Berry and Mikhail Popkov
Naive Antibody Libraries from Natural Repertoires
Claire L. Dobson, Ralph R. Minter, and Celia P. Hart-Shorrock
Synthetic Antibody Libraries
Frederic A. Fellouse and Sachdev S. Sidhu
Engineering Antibody Fragments for Intracellular Applications
Jianghai Liu and C. Ronald Geyer

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Editor(s)

Biography

Edited by

Sachdev S. Sidhu, PhD, professor, Department of Molecular Genetics, University of Toronto, Ontario, Canada

C. Ronald Geyer, PhD, professor, Department of Pathology, University of Saskatchewan, Canada

Reviews

“… The book covers broadly all aspects of phage-display technology and will be highly appreciated by those working with this technique and interested in applications. … The book is a clear invitation to use the phage-display technique both in biotechnology and drug discovery; … this is a very comprehensive book demonstrating the versatility and the value of phage display in many applications. It is clearly written giving an interesting historical background to many presently used variations on the phage-display technique.”
—Wim Quax, Rijksuniversiteit Groningen, The Netherlands, in ChemBioChem, Vol. 7, No. 699 - 703