Phage Display In Biotechnology and Drug Discovery: 2nd Edition (Paperback) book cover

Phage Display In Biotechnology and Drug Discovery

2nd Edition

Edited by Sachdev S. Sidhu, Clarence Ronald Geyer

CRC Press

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Description

Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies.

The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery.

Key Features:

  • Describes the basic principles of phage display and the methods and systems that have been developed for key applications
  • Outlines applications for peptide phage display in the development of vaccines and high- throughput screens as well as the exploration of protein–protein interactions and enzyme substrate characteristics
  • Details the use of phage display in the engineering of protein stability, the identification and mapping of protein–protein interactions, and the development of catalysts
  • Provides broad coverage of the impact of phage display technology on the development of protein therapeutics
  • Presents expert opinions on future challenges in the field

Reviews

“… The book covers broadly all aspects of phage-display technology and will be highly appreciated by those working with this technique and interested in applications. … The book is a clear invitation to use the phage-display technique both in biotechnology and drug discovery; … this is a very comprehensive book demonstrating the versatility and the value of phage display in many applications. It is clearly written giving an interesting historical background to many presently used variations on the phage-display technique.”

—Wim Quax, Rijksuniversiteit Groningen, The Netherlands, in ChemBioChem, Vol. 7, No. 699 - 703

Table of Contents

Filamentous Bacteriophage Structure and Biology

Diane J. Rodi, Suneeta Mandava, and Lee Makowski

Vectors and Modes of Display

Valery A. Petrenko and George P. Smith

Methods for the Construction of Phage-Displayed Libraries

Frederic A. Fellouse and Gabor Pal

Selection and Screening Strategies

Mark S. Dennis

Leveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell Surface

Jelena Tomic, Megan McLaughlin, Traver Hart, Sachdev Sidhu, and Jason Moffat

Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines

Nienke E. van Houten and Jamie K. Scott

Exploring Protein-Protein Interactions Using Peptide Libraries Displayed on Phage

Kurt Deshayes

Substrate Phage Display

Shuichi Ohkubo

Mapping Intracellular Protein Networks

Malgorzata E. Kokoszka, Zhaozhong Han, Ece Karatan, and Brian K. Kay

High-Throughput and High-Content Screening Using Peptides

Robert O. Carlson, Robin Hyde-Deruyscher, and Paul T. Hamilton

Engineering Protein Folding and Stability

Mihriban Tuna and Derek N. Woolfson

Identification of Natural Protein-Protein Interactions with cDNA Libraries

Reto Crameri, Claudio Rhyner, Michael Weichel, Sabine Fluckiger, and Zoltan Konthur

Mapping Protein Functional Epitopes

Sara K. Avrantinis and Gregory A. Weiss

Selections for Enzymatic Catalysts

Julian Bertschinger, Christian Heinis, and Dario Neri

Antibody Humanization and Affinity Maturation Using Phage Display

Jonathan S. Marvin and Henry B. Lowman

Antibody Libraries from Immunized Repertoires

Jody D. Berry and Mikhail Popkov

Naive Antibody Libraries from Natural Repertoires

Claire L. Dobson, Ralph R. Minter, and Celia P. Hart-Shorrock

Synthetic Antibody Libraries

Frederic A. Fellouse and Sachdev S. Sidhu

Engineering Antibody Fragments for Intracellular Applications

Jianghai Liu and C. Ronald Geyer

About the Editors

Edited by

Sachdev S. Sidhu, PhD, professor, Department of Molecular Genetics, University of Toronto, Ontario, Canada

C. Ronald Geyer, PhD, professor, Department of Pathology, University of Saskatchewan, Canada

About the Series

Drug Discovery Series

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MED072000
MEDICAL / Pharmacy
SCI013000
SCIENCE / Chemistry / General
SCI043000
SCIENCE / Research & Methodology