Piper is the representative genus of family Piperaceae. Piper species are pan-tropical in distribution and found in both the hemisphere. King of spices black pepper, Piper nigrum led to the global expeditions culminating in the discovery of India and the new world. Piper species has been reported to possess various pharmacological activities such as insecticidal, anti-bacterial, anti-inflammatory, anti-platelet, anti-hypertensive, anti-thyroid, anti-tumor activities and hepatoprotective properties. Botanical authentication of the plants of Piper species is difficult because of the morphological similarity among the species. This book describes ultra-performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry in multiple reactions monitoring (MRM) mode in the species to study the quantitative variation of thirteen bioactive markers in different plant parts of ten Piper species.
Introduction. Time Mass Spectrometry combined with Principal Component Analysis. Quantitative Determination of Chemical Constituents of Piper Species using UPLC–ESI–MS/MS. Conclusion. References.