1st Edition
Plant Biotechnology The Medicinal and Phytopharmaceutical Aspects
Chapter 1: Historical perspectives, Prospects for the Development of Plant Biotechnology as a Source of Medicinal Agents: Applications in Pharmacy and Allied FieldsDivya Sahu, Lokendra Singh Rathor, Manju Rawat Singh, Deependra Singh
Chapter 2: Requirements to Setting Up a Plant Biotechnology Laboratory and a General Laboratory
Shilpa Malakar, Priya Sutaoney, Durgesh Nandini Chauhan, Kamal Shah, and Nagendra Singh Chauhan
Chapter 3: Recombinant DNA Technology and Its Application in Plant Biotechnology
Jyoti, Preeti Garg, Vandana Garg, Rohit Dutt, Manju Rawat Singh, Deependra Singh, and Rahul Pratap Singh
Chapter 4: Protoplast Isolation and Fusion
Shalini Mathpal, Tushar Joshi, Priyanka Sharma, Priyanka Maiti, Mahesha Nand, and Subhash Chandra
Chapter 5: In vitro Propagation of Medicinal and Aromatic Plants: The Current Status on Concepts and Methods
Hemavathi Brijesh, Renuka Madhu, Brijesh Ajjappala, Ramya Manjunath, and Santosh Anand
Chapter 6: Sterilization Methods and Techniques Involved in Tissue Culture, with Protocols to Sterilize Different Plant Parts
Kiran, Vandana Garg, Rohit Dutt, Manju Rawat Singh, Deependra Singh, and Rahul Pratap Singh
Chapter 7: Gene Transfer in Plants and Their Application
Manish Kumar, Vichitra Kaushik, Manisha Bhatia, Janmejay Pant, A. Pandurangan, and Rupesh Kumar Gautam
Chapter 8: Immobilization Techniques of Plant Cells and Their Application on Secondary Metabolite Production
Jashanpreet Singh Sandhu, Vichitra Kaushik, Alakh Niranjan Sahu, Vipin Saini, Jeevan Jyoti Kaushik
Chapter 9: Cloning of Plant Cells: Different Methods of Cloning and Its Applications—Advantages and Disadvantages of Plant Cell Cloning
Zeeshan Khan, Aqsa Akram, Muhammad Irfan Khan, and Khushbukhat Khan
Chapter 10: Secondary Metabolism in Tissue Cultures with Emphasis on the Production of Medicinal Agents: Precursors and Elicitors on the Production of Secondary Metabolites—Applications and Associated Problems
Gajanand Engla, Love Kumar Soni, Mayur Chaurey, and Arun Patidar
Chapter 11: Biotransformation: Bioreactors for Pilot and Large-Scale Cultures of Plant Cells and Retention of Biosynthetic Potential in Cell Cultures
N. Joshi , G.Karri , and V. Joshi
Chapter 12: Biotransformation: Bioreactor Strategies for Scaling Plant Cell Cultures and Metabolic Stability
Devyani Rajput, Rahul Singh Thakur, Akanksha Dwivedi, G. N. Darwhekar, and Rakhi Khabiya
Chapter 13: Methods Used in Gene Identification, Localization, and Sequencing: Applications of PCR in Plant Genome Analysis
Ram Baran Singh, Ramachandra R, and Santosh Anand
Chapter 14: Fermentation Technology for the Production of Ergot Alkaloids: Single-Cell Proteins and Enzymes of Pharmaceutical Interest
Akash Jain, Jasmine Chaudhary, Vichitra Kaushik, Pankaj Dagur, Jashanpreet Singh Sandhu, and Manik Ghosh
Chapter 15: Computational Techniques for Drug Discovery from Medicinal Plants
Ajay Kumar Shukla, Joohee Pradhan, Manish Kumar, Sunita Panchawat, and C.P. Jain
Chapter 16: Biotechnological Approaches for the Microbial Production of Plant‐Based Compounds
Yuvaraj Sivamani, Hayagriba Swain, and Sumitha Elayaperumal
Chapter 17: DNA-Based Approaches for the Discrimination of Medicinal Plants from Their Adulterants
Yuvaraj Sivamani, Afrah Kounin P, and Sumitha Elayaperumal
Chapter 18: Medicinal Plants and Their Chemical Compounds in Ruminant Nutrition and Antimethanogenic Effects: The Biotechnological Approach
Edwin Rafael Alvarado-Ramírez, Mohamed Z.M. Salem, Ahmed Abdel-Megeed, Pedro Enrique Hernández-Ruiz, and Abdelfattah Z.M. Salem
Chapter 19: Isolation Techniques of Phytocompounds of Medicinal Plants
Dipak D. Gadade, Vimal Kumar, Ekta Gurnany, Sourabh Jain, Aakanchha Jain, and Anuj Modi
20. Potential Role of Metabolomics in Medicinal Plant Biotechnology Research
Devyani Rajput, Neha Sisodiya, Sampat Singh Tanwar, Rahul Singh Thakur, G. N. Darwhekar, and Rakhi Khabiya
Biography
Prof. Deependra Singh (M.Pharm, Ph.D, Post-doc (USA)) is presently working at University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.) India. He is chairman of the education regulation committee and central council member of the pharmacy council of India (PCI). He has around 20 years of teaching and research experience. He has 145 research and review papers published in national and international journals. He has written 30 book chapters and two books with Elsevier. He has successfully completed projects funded by ICMR, UGC, AICTE, DST, CGCOST, and several industrial projects. He has citations more than 7943 (h-index of 36 and i10 index of 123-Google scholar) and scopus h-index 30; 3514 citations. He has 2 international, 3 Indian patents and 2 filed. He has been among the top 2% of scientists across the world in the field of pharmacy and pharmacology in a survey by Stanford University, USA for four consecutive years (2021, 2022, 2023, 2024). He was a visiting scientist at the National Center for Natural Products Research, School of Pharmacy, University of Mississippi, USA. He was a recipient of the DHR-ICMR award for young biomedical scientist in 2019, UGC RAMAN international fellow in 2014, UGC research award in 2012, and a number of awards at national and state level for excellence in research. He is a life member of various professional bodies such as the Indian Pharmaceutical Association (IPA), Association of Pharmacy Teachers of India (APTI), Indian Society of Pharmacognosy (ISP), and serving as Vice-President, Central zone APTI 2022–2027 (Elected), Vice-President, Central zone IPGA 2022–2025 (Elected), Secretary, Chhattisgarh state branch IPA.
Durgesh Nandini Chauhan, M.Pharm., has several years of academic (teaching) experience at institutes in India in pharmaceutical sciences, and currently works in Columbia College of Pharmacy, Raipur CG India. She has taught subjects such as pharmaceutics, pharmacognosy, traditional concepts of medicinal plants, drug delivery, phytochemistry, cosmetic technology, pharmaceutical engineering, pharmaceutical packaging, quality assurance, dosage form designing, and anatomy and physiology. She is a member of the Association of Pharmaceutical Teachers of India, SILAE: Società Italo-Latinoamericana di Etnomedicina (The Scientific Network on Ethnomedicine, Italy), and others. She has attended several workshops, conferences, and symposiums, including the AICTE-Sponsored Staff Development Program on “Effects of Teaching and Learning Skills in Pharmacy: Tool for Improvement of Young Pharmacy Teachers.” She has written more than 10 articles published in national and international journals, 15 book chapters, and edited 10 books. She is also active as a reviewer for several international scientific journals and is an active participant at national and international conferences, including Bhartiya Vigyan Sammelan and the International Convention of Society of Pharmacognosy. Presently she has joined Ishita Research Organization, Raipur, India as a freelance writer and guide for students in pharmacy, Ayurvedic, and science in their research projects. She is interested in data analysis, proposal writing, questionnaire design, designing and developing research questionnaires and policy. She is actively engaged with accurately monitoring, investigating, and analyzing all statistical information, professionally presenting information and reports, and investigating and documenting key data and information at the request of senior researchers.
Dr. Nagendra Singh Chauhan obtained his M.Pharm, Ph.D. from the Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar, MP, India. He has around 20 years of research experience. He is presently working as a Senior Scientific Officer Grade-I and Government Analyst at Drugs Testing Laboratory Avam Anusandhan Kendra, Raipur, Chhattisgarh, India. He has professional expertise in Natural Product Isolation and phytopharmacology. He has written more than 70 articles published in national and international journals, and 32 book chapters, and edited 7 books. He is the recipient of the Dr. P. D. Sethi Memorial National Award for the best HPTLC publication from India in the years 2021 and 2022. He is a member of various professional and academic bodies such as the Society of Pharmacognosy India, the International Natural Product Sciences Taskforce (INPST), SILAE: Società Italo-Latinoamericana di Etnomedicina (The Scientific Network on Ethnomedicine, Italy), Institutional Human Ethical Committee, Association of Pharmaceutical Teachers of India (APTI), and Indian Pharmacy Graduates’ Association.
Prof. Manju Singh (M.Pharm, Ph.D) is presently working at University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.) India. She has around 20 years of teaching and research experience. She is among the top 2% of scientists across the world in the field of Pharmacy and Pharmacology surveyed by Stanford University, USA. She is engaged in research projects focused on studying the role of plant bioactives in devising new treatments mainly for autoimmune disorders and inflammatory diseases in collaboration with various universities in India and abroad. She has around 140 research and review papers published in national and international journals. She has written 30 book chapters and two books with Elsevier. She has citations more than 6867 with h-index 37, i10 index of 94 (Google scholar) and scopus h-index 30; 3525 citations. She has 2 international, 3 Indian patents and 2 filed. She has successfully completed several projects funded by the Indian Council of Medical Research (ICMR), University Grants Commission (UGC), C.G. State Council of Science and Technology (CGCOST), and the Department of Science and Technology (DST). She was a visiting scientist at the Department of Pharmaceutics, University of Mississippi, USA in 2015. She is the recipient of the DHR-ICMR international young Indian biochemist award 2014–15 and the APTI Prof. C.J. Shishoo Award for Research in Pharm Sciences 2024. She is a life member of professional bodies such as the Indian Pharmaceutical Association (IPA), Association of Pharmacy Teachers of India (APTI), Indian Pharmacy Graduates Association (IPGA), and serving as President, IPGA state branch Chhattisgarh 2022–2025 (Elected).
