Plant Chromosomes
Laboratory Methods
Preview
Book Description
Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies.
Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.
Table of Contents
Plant Chromosomes at Mitosis, K. Fukui
Outline of Mitotic Chromosomes
Preparation Method for the Small Chromosome: Enzymatic Maceration/Air Drying (EMA) Method
Preparation Method for the Large Chromosome: Squash Method
Preparation Method for Chromosomal Samples of Woody Plants
Preparation Method for Chromosome Samples of Suspension Cells
Preparation Method for Chromosome Samples of Flowers and Leaves
Permanent Preparation of Chromosome Samples
Plant Chromosomes at Meiosis, P.G. Goicoechea and R. Giraldez
Introduction
Meiotic Synchrony and Meiotic Gradients
Procedures
Plant Chromosomes at Metabolic Phase, K. Taniguchi
Introduction
Morphological Classification by Distribution of Condensed Heterochromatin
Visualizing Condensation
Application of Chromosome Banding Techniques
Polytene Chromosomes, W. Nagl
Introduction
Occurrence and Structure of Polytene Chromosomes in Plants
Preparation
Staining Methods
DNA and Gene Analysis
Digitation and Computer Analysis
Future Challenges
Flow Cytometry and Chromosome Sorting, J.S. Heslop-Harrison and T. Schwarzacher
Introduction
Procedures
Future Applications
Chromosome Dissection and Direct Cloning, Y. Kamisugi and K. Fukui
Introduction
Equipment
Microdissection of Plant Chromosomes
Direct Cloning
Direct-Labeling and In Situ Hybridization
Chromosome Banding Methods, B. Friebe, T.R. Endo, and B.S. Gill
Introduction
Principles of Chromosome Banding
Procedures
In Situ Hybridization, Y. Mukai
Introduction
Advances in In Situ Hybridization
Procedures
Sister Chromatid Exchange and Replication Banding, B. Friebe and F. Cortés
Introduction
Sister Chromatid Differentiation in Second Division Chromosomes
Sister Chromatid Differentiation in Third Division Chromosomes
Replication Banding
Procedures
Replication of Chromosomes, K. Taniguchi and I. Fujishige
Introduction
Principles of Replication Banding
Procedures
Applications
Chromosome Manipulation in Wheat, J.P. Gustafson and J.W. Snape
Introduction
Genome Balance and Introgression
Random Homologous Alien Transfers
Irradiation Technologies and Selection Strategies
Homologous Transfers by the Control of Recombination
Alien Introgression and Gene Expression
Conclusions
Electron Microscopy and Plant Chromosomes, J.S. Heslop-Harrison
Introduction
Developments in Electron Microscopy
General Precautions for Electron Microscopy
The Specimen
Procedure
DNA:DNA In Situ Hybridization
Quantification and Reconstruction of Three Dimensional Images from Serial Sections
Transfer of Spread Chromosome Preparations from Slides to Electron Microscope
Developments and Conclusions
Analysis of Chromosome Information, K. Fukui and S. Nakayama
Historical Perspective of Imaging Plant Chromosomes
Concept of Image Analyses
Instrumentation and Software
Procedure
Appendix I: Buffer Solutions
Appendix II: Tissue Culture Media
Index