Plant Chromosomes: Laboratory Methods, 1st Edition (Paperback) book cover

Plant Chromosomes

Laboratory Methods, 1st Edition

By Kiichi Fukui, Shigeki Nakayama

CRC Press

288 pages

Purchasing Options:$ = USD
Paperback: 9780849389191
pub: 1996-09-26
$175.00
x
eBook (VitalSource) : 9780203743195
pub: 2017-12-14
from $102.00


FREE Standard Shipping!

Description

Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies.

Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.

Table of Contents

Plant Chromosomes at Mitosis, K. Fukui

Outline of Mitotic Chromosomes

Preparation Method for the Small Chromosome: Enzymatic Maceration/Air Drying (EMA) Method

Preparation Method for the Large Chromosome: Squash Method

Preparation Method for Chromosomal Samples of Woody Plants

Preparation Method for Chromosome Samples of Suspension Cells

Preparation Method for Chromosome Samples of Flowers and Leaves

Permanent Preparation of Chromosome Samples

Plant Chromosomes at Meiosis, P.G. Goicoechea and R. Giraldez

Introduction

Meiotic Synchrony and Meiotic Gradients

Procedures

Plant Chromosomes at Metabolic Phase, K. Taniguchi

Introduction

Morphological Classification by Distribution of Condensed Heterochromatin

Visualizing Condensation

Application of Chromosome Banding Techniques

Polytene Chromosomes, W. Nagl

Introduction

Occurrence and Structure of Polytene Chromosomes in Plants

Preparation

Staining Methods

DNA and Gene Analysis

Digitation and Computer Analysis

Future Challenges

Flow Cytometry and Chromosome Sorting, J.S. Heslop-Harrison and T. Schwarzacher

Introduction

Procedures

Future Applications

Chromosome Dissection and Direct Cloning, Y. Kamisugi and K. Fukui

Introduction

Equipment

Microdissection of Plant Chromosomes

Direct Cloning

Direct-Labeling and In Situ Hybridization

Chromosome Banding Methods, B. Friebe, T.R. Endo, and B.S. Gill

Introduction

Principles of Chromosome Banding

Procedures

In Situ Hybridization, Y. Mukai

Introduction

Advances in In Situ Hybridization

Procedures

Sister Chromatid Exchange and Replication Banding, B. Friebe and F. Cortés

Introduction

Sister Chromatid Differentiation in Second Division Chromosomes

Sister Chromatid Differentiation in Third Division Chromosomes

Replication Banding

Procedures

Replication of Chromosomes, K. Taniguchi and I. Fujishige

Introduction

Principles of Replication Banding

Procedures

Applications

Chromosome Manipulation in Wheat, J.P. Gustafson and J.W. Snape

Introduction

Genome Balance and Introgression

Random Homologous Alien Transfers

Irradiation Technologies and Selection Strategies

Homologous Transfers by the Control of Recombination

Alien Introgression and Gene Expression

Conclusions

Electron Microscopy and Plant Chromosomes, J.S. Heslop-Harrison

Introduction

Developments in Electron Microscopy

General Precautions for Electron Microscopy

The Specimen

Procedure

DNA:DNA In Situ Hybridization

Quantification and Reconstruction of Three Dimensional Images from Serial Sections

Transfer of Spread Chromosome Preparations from Slides to Electron Microscope

Developments and Conclusions

Analysis of Chromosome Information, K. Fukui and S. Nakayama

Historical Perspective of Imaging Plant Chromosomes

Concept of Image Analyses

Instrumentation and Software

Procedure

Appendix I: Buffer Solutions

Appendix II: Tissue Culture Media

Index

Subject Categories

BISAC Subject Codes/Headings:
SCI011000
SCIENCE / Life Sciences / Botany
SCI029000
SCIENCE / Life Sciences / Genetics & Genomics
SCI086000
SCIENCE / Life Sciences / General