Plant Chromosomes : Laboratory Methods book cover
1st Edition

Plant Chromosomes
Laboratory Methods

ISBN 9780849389191
Published September 26, 1996 by CRC Press
286 Pages

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Book Description

Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies.
Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.

Table of Contents

Plant Chromosomes at Mitosis, K. Fukui
Outline of Mitotic Chromosomes
Preparation Method for the Small Chromosome: Enzymatic Maceration/Air Drying (EMA) Method
Preparation Method for the Large Chromosome: Squash Method
Preparation Method for Chromosomal Samples of Woody Plants
Preparation Method for Chromosome Samples of Suspension Cells
Preparation Method for Chromosome Samples of Flowers and Leaves
Permanent Preparation of Chromosome Samples
Plant Chromosomes at Meiosis, P.G. Goicoechea and R. Giraldez
Meiotic Synchrony and Meiotic Gradients
Plant Chromosomes at Metabolic Phase, K. Taniguchi
Morphological Classification by Distribution of Condensed Heterochromatin
Visualizing Condensation
Application of Chromosome Banding Techniques
Polytene Chromosomes, W. Nagl
Occurrence and Structure of Polytene Chromosomes in Plants
Staining Methods
DNA and Gene Analysis
Digitation and Computer Analysis
Future Challenges
Flow Cytometry and Chromosome Sorting, J.S. Heslop-Harrison and T. Schwarzacher
Future Applications
Chromosome Dissection and Direct Cloning, Y. Kamisugi and K. Fukui
Microdissection of Plant Chromosomes
Direct Cloning
Direct-Labeling and In Situ Hybridization
Chromosome Banding Methods, B. Friebe, T.R. Endo, and B.S. Gill
Principles of Chromosome Banding
In Situ Hybridization, Y. Mukai
Advances in In Situ Hybridization
Sister Chromatid Exchange and Replication Banding, B. Friebe and F. Cort├ęs
Sister Chromatid Differentiation in Second Division Chromosomes
Sister Chromatid Differentiation in Third Division Chromosomes
Replication Banding
Replication of Chromosomes, K. Taniguchi and I. Fujishige
Principles of Replication Banding
Chromosome Manipulation in Wheat, J.P. Gustafson and J.W. Snape
Genome Balance and Introgression
Random Homologous Alien Transfers
Irradiation Technologies and Selection Strategies
Homologous Transfers by the Control of Recombination
Alien Introgression and Gene Expression
Electron Microscopy and Plant Chromosomes, J.S. Heslop-Harrison
Developments in Electron Microscopy
General Precautions for Electron Microscopy
The Specimen
DNA:DNA In Situ Hybridization
Quantification and Reconstruction of Three Dimensional Images from Serial Sections
Transfer of Spread Chromosome Preparations from Slides to Electron Microscope
Developments and Conclusions
Analysis of Chromosome Information, K. Fukui and S. Nakayama
Historical Perspective of Imaging Plant Chromosomes
Concept of Image Analyses
Instrumentation and Software
Appendix I: Buffer Solutions
Appendix II: Tissue Culture Media

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