Cytogenetics plays an important role in understanding the chromosomal and genetic architecture of plant species. Plant Cytogenetics, Third Edition follows the tradition of its predecessors presenting theoretical and practical aspects of plant cytogenetics. Chapters describe correct handling of plant chromosomes, methods in plant cytogenetics, cell division, reproduction methods, chromosome nomenclature, karyotype analysis, chromosomal aberrations, genome analysis, transgenic crops, and cytogenetics in plant breeding.
This new edition begins with a brief introduction on the historical aspect of cytogenetics and flows directly into handling of plant chromosomes by classical and modern cytological techniques, classical Mendelian Genetics, brief description of cell division, and chromosome identification by karyotype analysis. The comprehension of cytogenetics is incomplete without information on the role of aneuploidy in associating a gene on a particular chromosome, and the book covers these methodologies as a primary topic. Covering classical to modern cytogenetics, the book presents to the reader the crucial role of cytogenetics in improving crops.
Table of Contents
THE HANDLING OF PLANT CHROMOSOMES
Squash Technique - Mitotic and Meiotic Chromosomes
Smear Technique for Plant Chromosomes
Fluorescence in situ Hybridization (FISH)
Total DNA Extraction-Plant Genomic DNA
Determination of Nuclear DNA Content of Plants by Flow Cytometry
Karyotyping and Sorting of Plant Chromosomes by Flow Cytometry
Production of Wide Hybrids Through In Vitro Technique
GENETIC CONTROL OF MEIOSIS
Genetic Control of Recombination
Genes Responsible for Chromosome Disjunction
Other Meiotic Mutants
Role of Heterochromatin in Chromosome Pairing
Diploid-like Meiosis in Allopolyploids
MODE OF REPRODUCTION IN PLANTS
The Chromosomal Basis of Sex Determination in Plants
Nomenclature of Chromosomes
Karyotype Analysis by Mitotic Metaphase Chromosomes
Karyotype Analysis by Pachytene Chromosomes
Karyotype Analysis by Flow Cytometry
Karyotype Analysis by Image Analysis
Chromosome Number of Economically Important Plants
CHROMOSOMAL ABERRATIONS - STRUCTURAL CHROMOSOME CHANGES
CHROMOSOMAL ABERRATIONS - NUMERICAL CHROMOSOME CHANGES
Monosomics and Nullisomics
Alien Addition Lines
Alien Substitution Lines
Classical Taxonomy and Genome Designations
Genome Relationships among Diploid Species
Genomes of Glycine
Genomes of Gossypium
Ancestors of Allopolyploid Species
Genome Analysis by Molecular Methods
CHROMOSOMAL ABERRATIONS IN CELL AND TISSUE CULTURE
Chromosomal Aberrations in Callus
Mechanism of Chromosomal Aberrations in Culture
Chromosomal Aberrations in Regenerated Plants
Chromosomal Aberrations in Haploid Callus and Their Regenerants
Chromosomal Aberrations in Somatic Hybrids
Present Status of Transgenic Crops
Production of Transgenic Crops
Chromosome Aberrations in Transgenic Crops
Cytological Basis of Gene Silencing
"Plant Cytogenetics" by Ram Singh gives an outstanding treatment of classical cytogenetics and then ably integrates molecular cytogenetic approaches. While commercial crops have been studied in the greatest detail cytogenetically, the species are treated not in specialized chapters but under cytogenetic principles. A mastery of the cytogenetic phenomena that has been reported for these crops, such as structural and numerical chromosome analyses (Chapter 7) and genome analysis with examples from Glycine, Gossypium, Brassica, Nicotiana and Triticum (Chapter 8) enable one to be completely familiar with the mechanisms of cytogenetics. Many Tables will be found especially useful, such as the list of primary trisomics derived from autotriploids, and the alien chromosome substitution list giving plant recipient, donor, procedure for transfer and genes transferred. …
There is an excellent Glossary of 25 pages and a list of References comprising 58 pages. An extensive Index serves both as a subject matter and a species index. The text is quite adequately illustrated with 114 figures. …"Plant Cytogenetics" is a solid text embracing both classical and molecular aspects of cytogenetics. The Chapter on cytogenetic techniques will make the text a classic in its own right. In conclusion, I highly recommend the text for a thorough understanding of the basic mechanisms of plant cytogenetics for which the text is primarily designed.
- William F. Grant, Department of Plant Science, McGill University, Quebec, Canada
"For advanced students who are looking for descriptions of the mechanics of the wide variety of chromosomal aberrations that have been studied in crop plants, this text should be of considerable use."
-Plant Science Bulletin on the first edition
"… provides an excellent review of various techniques in handling of chromosomes, karyotype analysis, genetics of meiosis, genomic relationships, and chromosome manipulations. In addition to his own studies, Dr. Singh has made extensive use of the information published in various journals…The contents of the book are well arranged and easy to read and understand. It should serve as a useful textbook for students taking courses in genetics and cytogenetics and as a reference book for scientists engaged in plant cytogenetic research."
-Theoretical and Applied Genetic on the first edition s
"This is a solid text…I recommend the text for a thorough understanding of the basic mechanisms of plant cytogenetics for which the text is primarily designed."
"This is a comprehensive handbook that deals both with the theoretical and practical aspects related to plant cytogenetics and will be a valuable reference work for those entering the field for the first time, those who occasionally venture into the field, plant breeders, and those involved in active research."
– Glendon Ascough, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, in South African Journal of Botany, 2007, Vol. 73, No. 3