1st Edition
Plant Tissue Culture Principles, Tools, and Techniques
Preface
1. Introduction to Plant Tissue Culture
Introduction
The Early Years of Plant Tissue Culture
Milestones in the Development of the Techniques of Plant Tissue Culture
Contribution of Indian and Overseas Scientists to Plant Tissue Culture
Recent Past of Plant Tissue Culture
The Present Era of Plant Tissue Culture
Plant Tissue Culture/Cell Products Entrepreneurship in the 21st Century
2. Laboratory Facilities and Operations for Plant Tissue Culture
Introduction
Design and Layout of Tissue Culture Laboratory
Washing Area
Media Preparation Area
Sterilization Room
Aseptic Transfer Area/Inoculation Room
Culture Room/Growth Room
Data Collection Area
Acclimatization Area
Cold Storage, Shipping/Lunch Area, Offices and Miscellaneous Storage
Safety Rules for Plant Tissue Culture
Automation in Plant Tissue Culture
3. Nutrient Media Formulations for Plant Tissue Cultures
Introduction
Media Constituents
Liquid Media
Commonly Used Basal Media
pH of the Media
Antibiotics
Media Preparation
4. Culture Contamination: Troubleshooting and Prevention
What is Microbial Contamination?
Various Sources of Contamination
Disinfectant Agents
Prevention of Microbial Contamination
5. In Vitro Culture Initiation
Introduction
Effect of Different Factors on Explant Regeneration
Explant Preparation
6. Plant Tissue Culture Techniques
Introduction
Somatic Embryogenesis (SE)
Somatic Embryogenesis for Synthetic Seed Productions
Organogenesis and Regeneration
Somaclonal Variation and Micropropagation
Plant Protoplast Isolation and Fusion
Cell Suspension Cultures
Haploid Plants from Anther Culture
Meristem Culture for Virus-free Plants
Isolation of the Shoot Apical Meristem
Applications of Plant Tissue Culture
Introduction
Conservation of Rare, Endangered, and Threatened (RET) Medicinal, Aromatic, and Endemic Plants
Cell Suspension Culture for the Production of Desired Secondary Metabolites
Hairy Root Cultures for Enhanced Production of Secondary Metabolites
Application of Plant Tissue Culture in Plant Breeding
Micropropagation
Introduction
Explants for Micropropagation
Methods of Plantlet Production
Steps Involved in Micropropagation
Banana Micropropagation
Economic Significance
Applications of Micropropagation
Appendix
Index
Authors’ Biography
Biography
Mr. Surendra R. Sinkar holds a Master of Science degree in Biotechnology from Sant Gadge Baba Amravati University. He is an Assistant Professor in the Department of Botany at Arvindbabu Deshmukh Mahavidyalaya Bharsingi, affiliated with RTM Nagpur University. He has experience as a Research Associate at Bejo Sheetal Seeds Pvt. Ltd., where he worked on plant tissue culture and transgenic crops. He has published 8 research papers and 1 book chapter and is passionate about plant biotechnology education.
Dr. Mahendra Rai is a Senior Visiting Professor at the Department of Chemistry, Federal University of Piauí, Brazil. Formerly, he served as a Professor and Head of Biotechnology at SGB Amravati University, India. He has held visiting scientist positions at prestigious institutions, including the University of Geneva, Debrecen University, Hungary and the University of Campinas, Brazil. With over 425 research papers, 70+ books, and six patents, Dr. Rai is recognized as a leading expert in his field. He has been featured on Stanford’s top 2% scientists list in Nanoscience.
