1st Edition

Plant Transformation via Agrobacterium Tumefaciens Culture Conditions, Recalcitrance and Advances in Soybean

By Phetole Mangena Copyright 2023
    244 Pages 29 Color & 2 B/W Illustrations
    by CRC Press

    244 Pages 29 Color & 2 B/W Illustrations
    by CRC Press

    244 Pages 29 Color & 2 B/W Illustrations
    by CRC Press

    Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes.

    This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment.

    Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

    Overview of Genetic Transformation in Plants

    Plant Transformation History

    Agrobacterium Tumefaciens

    Getting Started with In Vitro Soybean Transformation

    In Vitro Cultures Commonly used for Plant Transformation

    Current and Longstanding Challenges facing Soybean Transformation

    Alternative Techniques for Genetic Manipulations via A. Tumefaciens

    Molecular Aspects of Indirect Gene Transfer via A. Tumefaciens

    Genetic Transformation in Other Leguminous Crops

    Transgenic Plants for Biotic Stress Resistance

    Transgenic Plants for Abiotic Stress Resistance

    Potential Health Safety Concerns and Environmental Risks

    The Regulatory Management of Transgenic Plants

    Current and Future Prospects in Genetic Engineering

    Photographic Index of Soybean Transformation Cultures


    Phetole Mangena obtained his PhD degree in Botany in the field of plant physiology and biotechnology. He is a senior lecturer and researcher in the Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture at the University of Limpopo, Republic of South Africa. Dr Mangena’s work involve micropropagation via tissue culture, crop improvement and breeding through mutagenic chemicals as well as Agrobacterium-mediated genetic transformation. He has authored and co-authored numerous scientific papers and book chapters related to genetic transformation and morpho-physiological responses of plants to stress. Dr. Mangena is the sole editor of the Book: Advances in Legume Research- Physiological Responses and Genetic Improvement for Stress Resistance, published in 2020 (Volume 1) by Bentham Science Publishers Pte. Ltd, Singapore. He currently serves as the editor for the Scopus indexed Research Journal of BioTechnology and a fellow-member of the World Researchers Association. He is also a board member of the New Plant Biotechnology Persa Journal, member of the South African Association of Botanists and the International Society for Horticultural Science. Dr. Mangena has received numerous honours and awards, including 1st prize in the Innovation Hub GAP Bioscience Competition and the Research Excellence Award by the National Research Foundation of South Africa.