Practical Handbook of Microbiology  book cover
4th Edition

Practical Handbook of Microbiology

ISBN 9780367567637
Published May 5, 2021 by CRC Press
975 Pages 34 Color & 27 B/W Illustrations

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Book Description

Practical Handbook of Microbiology, 4th edition provides basic, clear and concise knowledge and practical information about working with microorganisms. Useful to anyone interested in microbes, the book is intended to especially benefit four groups: trained microbiologists working within one specific area of microbiology; people with training in other disciplines, and use microorganisms as a tool or "chemical reagent"; business people evaluating investments in microbiology focused companies; and an emerging group, people in occupations and trades that might have limited training in microbiology, but who require specific practical information.

Key Features

  • Provides a comprehensive compendium of basic information on microorganisms—from classical microbiology to genomics.
  • Includes coverage of disease-causing bacteria, bacterial viruses (phage), and the use of phage for treating diseases, and added coverage of extremophiles.
  • Features comprehensive coverage of antimicrobial agents, including chapters on anti-fungals and anti-virals.
  • Covers the Microbiome, gene editing with CRISPR, Parasites, Fungi, and Animal Viruses.
  • Adds numerous chapters especially intended for professionals such as healthcare and industrial professionals, environmental scientists and ecologists, teachers, and businesspeople.
  • Includes comprehensive survey table of Clinical, Commercial, and Research-Model bacteria.


The Open Access version of this book, available at, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license.

Chapter 21, "Archaea," of this book is freely available as a downloadable Open Access PDF under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license available at

See Emanuel Goldman's Open Access article: "Lamarck redux and other false arguments against SARS-CoV-2 vaccination,"

Table of Contents


About the Editors



1. Sterilization, Disinfection, and Antisepsis

Michael G. Schmidt

2. Quantitation of Microorganisms

Brad A. Slominski and Peter S. Lee

3. Culturing and Preserving Microorganisms

Lorrence H. Green

4. Stains for Light Microscopy

Stuart Chaskes and Rita Austin

5. Identification of Gram-Positive Organisms

Peter M. Colaninno

6. Identification of Aerobic Gram-Negative Bacteria

Donna J. Kohlerschmidt, Lisa A. Mingle, Nellie B. Dumas, and Geetha Nattanmai

7. Plaque Assay for Bacteriophage

Emanuel Goldman

8. Phage Identification of Bacteria

Catherine E.D. Rees and Martin J. Loessner

9. Phage Display and Selection of Protein Ligands

Geir Åge Løset, Wlodek Mandecki, and Inger Sandlie

10. Diagnostic Medical Microbiology

Lorrence H. Green

11. Modern Diagnostic Methods in the 21st Century

Lorrence H. Green and Alan Ward

12. Antibiotic Susceptibility Testing

Audrey Wanger and Violeta Chávez

13. Bacterial Cell Breakage or Lysis

Matthew E. Bahamonde

14. Major Culture Collections and Sources

Lorrence H. Green

15. Epidemiological Methods in Microbiology

Tyler S. Brown, Barun Mathema, and D. Ashley Robinson


Tao Xu, Megan L. Kempher, Xuanyu Tao, Aifen Zhou, and Jizhong Zhou


17. Taxonomic Classification of Bacteria

J. Michael Janda

18. Bacterial Cell Wall: Morphology and Biochemistry

Stefania De Benedetti, Jed F. Fisher, and Shahriar Mobashery

19. The Human Microbiome in Health and Disease

Sandra B. Andersen, Menghan Liu, and Martin J. Blaser

20. The Phylum Actinobacteria

Alan C Ward, Nagamani Bora, Jenileima Devi, Alexander Escasinas, and Nicholas Allenby

21. Archaea

Nina Dombrowski, Tara Mahendrarajah, Sarah T. Gross, Laura Eme, and Anja Spang

22. The Genus Bacillus

Daniel R. Zeigler and John B. Perkins

23. The Genus Bordetella

Rita Austin and Tonya Shearin-Patterson

24. The Genus Campylobacter

Collette Fitzgerald, Janet Pruckler, Maria Karlsson, and Patrick Kwan

Updated 2021: Janet Pruckler, Lavin Joseph, Hayat Caidi,

Mark Laughlin, Rachael D. Aubert

25. Chlamydiae

Lourdes G. Bahamonde

26. The Genus Clostridium

Peter Dürre

27. The Genus Corynebacterium

Lothar Eggeling and Michael Bott

28. The Family Enterobacteriaceae

J. Michael Janda and Denise L. Lopez

29. Haemophilus Species

Elisabeth Adderson

30. The Genus Helicobacter

Ernestine M. Vellozzi and Edmund R. Giugliano

31. The Genus Legionella

Ashley M. Joseph and Stephanie R. Shames

32. The Genus Listeria

Sukhadeo Barbuddhe, Torsten Hain, Swapnil P. Doijad, and Trinad Chakraborty

33. The Genus Mycobacterium

Leen Rigouts and Sari Cogneau

34. Mycoplasma and Related Organisms

Bahman Rostama and Meghan May

35. The Family Neisseriaceae

Yvonne A. Lue

36. The Genus Pseudomonas

Layla Ramos-Hegazy, Shubham Chakravarty, and Gregory G. Anderson

37. The Family Rickettsiaceae

Timothy P. Driscoll, Victoria I. Verhoeve, Magda Beier-Sexton, Abdu F. Azad, and Joseph J. Gillespie

38. Microbiological and Clinical Aspects of the Pathogenic Spirochetes

Charles S. Pavia

39. Staphylococcus aureus and Related Staphylococci

Volker Winstel, Olaf Schneewind, and Dominique Missiakas

40. Streptococcus

Vincent A. Fischetti and Patricia Ryan

41. The Genus Vibrio and Related Genera

Seon Young Choi, Anwar Huq, and Rita R. Colwell

42. Yersinia

Ryan F. Relich and Meghan A. May

43. Other Anaerobic Bacteria: Bacteroides, Porphyromonas, Prevotella, Tannerella, Fusobacterium, and Gram-positive Anaerobic Cocci

Joseph J. Zambon and Violet I. Haraszthy

44. Other Gram-Negative Bacteria: Acinetobacter, Burkholderia, and Moraxella

Rebecca E. Colman and Jason W. Sahl

45. Selected Zoonotic Pathogens

Sanjay K. Shukla and Steven Foley

46. Fungi

Charles Adair

47. Introduction to Parasites

Purnima Bhanot

48. Introduction to Bacteriophages

Elizabeth Kutter and Emanuel Goldman

49. Introduction to Virology

Ken S. Rosenthal

50. Emerging Viruses

Meghan A. May and Ryan F. RelichContents


51. Mechanisms of Action of Antibacterial Agents

Ammara Mushtaq, Joseph Adrian L. Buensalido, Carmen E. DeMarco, Rimsha Sohail, and Stephen A. Lerner

52. Mechanisms of Action of Antifungal Agents

Jeffrey M. Rybak and P. David Rogers

53. Mechanisms of Action of Antiviral Agents

Guido Antonelli, Francesca Falasca, and Ombretta Turriziani

54. Phage Therapy: Bacteriophages as Natural, Self-Replicating Antimicrobials

Naomi Hoyle and Elizabeth Martin Kutter

55. Emergence of Antimicrobial Resistance in Hospitals

Paramita Basu, Joshua Garcia, and Priyank Kumar

56. Emerging Antimicrobial-Resistant Microorganisms in the Community

Negin Alidazeh Shaygh, Divya Sarvaiya, and Paramita Basu

57. Overview of Biofilms and Some Key Methods for Their Study

Paramita Basu, Michael Boadu, and Irvin N. Hirshfield

58. Biofilms in Healthcare

Rebecca K. Kavanagh, Arindam Mitra, and Paramita Basu

59. The Business of Microbiology

Michael C. Nugent and Lorrence H. Green

60. Launching a Microbiology-Based Company

Leonard Osser

61. Microbiology for Dental Hygienists

Victoria Benvenuto and Donna L Catapano

62. Microbiology for Pre-College Teachers

Madge Nanney and Scott Sowell

63. Microbiology for Home Inspectors

William E. Herrmann

Survey of Selected Clinical, Commercial, and Research-Model Eubacterial Species


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Edited by

Emanuel Goldman is a professor in the Department of Microbiology, Biochemistry, and Molecular Genetics of the New Jersey Medical School (NJMS), Rutgers Biomedical and Health Sciences (RBHS), a division of Rutgers University, Newark, New Jersey. He graduated with honors from the Bronx High School of Science in 1962, received a BA (cum laude) from Brandeis University in 1966, where he was a chemistry major and music minor, and completed his PhD in biochemistry at the Massachusetts Institute of Technology in 1972. He performed postdoctoral research at Harvard Medical School and at the University of California, Irvine, before joining the faculty of the New Jersey Medical School in 1979, where he rose through the ranks to professor in 1993. Among his awards and honors, Dr. Goldman was a Damon Runyon fellow, a Lievre senior fellow of the California Division, American Cancer Society, and a recipient of the Research Career Development Award from the National Cancer Institute.

Among his service activities, he was an officer and organizer of the New York–New Jersey Molecular Biology Club, served as a full member of an American Cancer Society Study Section, and continues to serve on the editorial boards of Protein Expression and Purification and Applied and Environmental Microbiology. He was also twice elected by his colleagues to serve as the president of his university’s chapter of the American Association of University Professors, and he was elected to serve as president of the Faculty Organization of NJMS. Among several areas of research activity, he has focused on the role of tRNA in the elongation of bacterial protein synthesis, including uncharged tRNA, codon bias, and programmed translational frameshifts. In addition to numerous scientific peer-reviewed publications and publications in the lay press, he has contributed a chapter to Zubay’s Biochemistry textbook and four chapters to the Encyclopedia of Life Sciences. His recently published Comment in Lancet, "Exaggerated risk of transmission of COVID-19 by Fomites", has attracted significant international attention.

Lorrence H. Green, Ph.D., President of Westbury Diagnostics, Inc. earned his PhD in Cell and Molecular Biology from Indiana University, Bloomington, Indiana, in 1978. He followed this with three years of recombinant DNA and genetic research at Harvard University. In 1981, he moved into Industry by joining Analytab Products Inc., a major manufacturer of in vitro diagnostic test kits. During the next twelve years he helped to invent and manufacture over 40 diagnostic test kits, and rose to become the Director of New Product Development and Product Support.

In 1993, Dr. Green founded Westbury Diagnostics, Inc., a microbiology-biotechnology based contract research and development laboratory also offering consulting services. Mixing his love of business with his love of teaching Dr. Green has served as an adjunct associate professor of microbiology at the NY College of Osteopathic Medicine, and is currently an Adjunct Assistant Professor of Biology at Farmingdale State College and a Director of the Fundamentals of the Bioscience Industry Program at Stony Brook University of the State University of New York.

Dr. Green is on the steering committee, and is a former Chairman, of the Microbiology Section of the NY Academy of Sciences. He was also the long time Treasurer of the NYC Branch of the ASM. From 2001 until 2004 he was a member of the Advisory Committee on Emerging Pathogens and Bioterrorism to the New York City Commissioner of Health. In 2013 he was appointed to the Board of Directors of the Long Island Advancement of Small Business.

His main interests involve using technology in the development of commercial products and in being an entrepreneur who invests in and develops companies. He enjoys providing mentorship and career advice to students at all levels. He has spoken at many career day events, judged many regional science fairs, and has helped dozens of young people with applications to medical school, nursing school, physician’s assistant school, and with starting companies. Most recently he has become involved in government and is currently the Chairman of the Town of Mamakating Planning Board.