4th Edition
Practical Handbook of Microbiology
Practical Handbook of Microbiology, 4th edition provides basic, clear and concise knowledge and practical information about working with microorganisms. Useful to anyone interested in microbes, the book is intended to especially benefit four groups: trained microbiologists working within one specific area of microbiology; people with training in other disciplines, and use microorganisms as a tool or "chemical reagent"; business people evaluating investments in microbiology focused companies; and an emerging group, people in occupations and trades that might have limited training in microbiology, but who require specific practical information.
Key Features
- Provides a comprehensive compendium of basic information on microorganisms—from classical microbiology to genomics.
- Includes coverage of disease-causing bacteria, bacterial viruses (phage), and the use of phage for treating diseases, and added coverage of extremophiles.
- Features comprehensive coverage of antimicrobial agents, including chapters on anti-fungals and anti-virals.
- Covers the Microbiome, gene editing with CRISPR, Parasites, Fungi, and Animal Viruses.
- Adds numerous chapters especially intended for professionals such as healthcare and industrial professionals, environmental scientists and ecologists, teachers, and businesspeople.
- Includes comprehensive survey table of Clinical, Commercial, and Research-Model bacteria.
The Open Access version of this book, available at http://www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license.
Chapter 21, "Archaea," of this book is freely available as a downloadable Open Access PDF under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license available at http://www.taylorfrancis.com
See Emanuel Goldman's Open Access article: "Lamarck redux and other false arguments against SARS-CoV-2 vaccination," https://www.embopress.org/doi/full/10.15252/embr.202254675
Preface
About the Editors
Contributors
PART I. PRACTICAL INFORMATION AND PROCEDURES
1. Sterilization, Disinfection, and Antisepsis
Michael G. Schmidt
2. Quantitation of Microorganisms
Brad A. Slominski and Peter S. Lee
3. Culturing and Preserving Microorganisms
Lorrence H. Green
4. Stains for Light Microscopy
Stuart Chaskes and Rita Austin
5. Identification of Gram-Positive Organisms
Peter M. Colaninno
6. Identification of Aerobic Gram-Negative Bacteria
Donna J. Kohlerschmidt, Lisa A. Mingle, Nellie B. Dumas, and Geetha Nattanmai
7. Plaque Assay for Bacteriophage
Emanuel Goldman
8. Phage Identification of Bacteria
Catherine E.D. Rees and Martin J. Loessner
9. Phage Display and Selection of Protein Ligands
Geir Åge Løset, Wlodek Mandecki, and Inger Sandlie
10. Diagnostic Medical Microbiology
Lorrence H. Green
11. Modern Diagnostic Methods in the 21st Century
Lorrence H. Green and Alan Ward
12. Antibiotic Susceptibility Testing
Audrey Wanger and Violeta Chávez
13. Bacterial Cell Breakage or Lysis
Matthew E. Bahamonde
14. Major Culture Collections and Sources
Lorrence H. Green
15. Epidemiological Methods in Microbiology
Tyler S. Brown, Barun Mathema, and D. Ashley Robinson
16. CRISPR
Tao Xu, Megan L. Kempher, Xuanyu Tao, Aifen Zhou, and Jizhong Zhou
PART II. SURVEY OF MICROORGANISMS
17. Taxonomic Classification of Bacteria
J. Michael Janda
18. Bacterial Cell Wall: Morphology and Biochemistry
Stefania De Benedetti, Jed F. Fisher, and Shahriar Mobashery
19. The Human Microbiome in Health and Disease
Sandra B. Andersen, Menghan Liu, and Martin J. Blaser
20. The Phylum Actinobacteria
Alan C Ward, Nagamani Bora, Jenileima Devi, Alexander Escasinas, and Nicholas Allenby
21. Archaea
Nina Dombrowski, Tara Mahendrarajah, Sarah T. Gross, Laura Eme, and Anja Spang
22. The Genus Bacillus
Daniel R. Zeigler and John B. Perkins
23. The Genus Bordetella
Rita Austin and Tonya Shearin-Patterson
24. The Genus Campylobacter
Collette Fitzgerald, Janet Pruckler, Maria Karlsson, and Patrick Kwan
Updated 2021: Janet Pruckler, Lavin Joseph, Hayat Caidi,
Mark Laughlin, Rachael D. Aubert
25. Chlamydiae
Lourdes G. Bahamonde
26. The Genus Clostridium
Peter Dürre
27. The Genus Corynebacterium
Lothar Eggeling and Michael Bott
28. The Family Enterobacteriaceae
J. Michael Janda and Denise L. Lopez
29. Haemophilus Species
Elisabeth Adderson
30. The Genus Helicobacter
Ernestine M. Vellozzi and Edmund R. Giugliano
31. The Genus Legionella
Ashley M. Joseph and Stephanie R. Shames
32. The Genus Listeria
Sukhadeo Barbuddhe, Torsten Hain, Swapnil P. Doijad, and Trinad Chakraborty
33. The Genus Mycobacterium
Leen Rigouts and Sari Cogneau
34. Mycoplasma and Related Organisms
Bahman Rostama and Meghan May
35. The Family Neisseriaceae
Yvonne A. Lue
36. The Genus Pseudomonas
Layla Ramos-Hegazy, Shubham Chakravarty, and Gregory G. Anderson
37. The Family Rickettsiaceae
Timothy P. Driscoll, Victoria I. Verhoeve, Magda Beier-Sexton, Abdu F. Azad, and Joseph J. Gillespie
38. Microbiological and Clinical Aspects of the Pathogenic Spirochetes
Charles S. Pavia
39. Staphylococcus aureus and Related Staphylococci
Volker Winstel, Olaf Schneewind, and Dominique Missiakas
40. Streptococcus
Vincent A. Fischetti and Patricia Ryan
41. The Genus Vibrio and Related Genera
Seon Young Choi, Anwar Huq, and Rita R. Colwell
42. Yersinia
Ryan F. Relich and Meghan A. May
43. Other Anaerobic Bacteria: Bacteroides, Porphyromonas, Prevotella, Tannerella, Fusobacterium, and Gram-positive Anaerobic Cocci
Joseph J. Zambon and Violet I. Haraszthy
44. Other Gram-Negative Bacteria: Acinetobacter, Burkholderia, and Moraxella
Rebecca E. Colman and Jason W. Sahl
45. Selected Zoonotic Pathogens
Sanjay K. Shukla and Steven Foley
46. Fungi
Charles Adair
47. Introduction to Parasites
Purnima Bhanot
48. Introduction to Bacteriophages
Elizabeth Kutter and Emanuel Goldman
49. Introduction to Virology
Ken S. Rosenthal
50. Emerging Viruses
Meghan A. May and Ryan F. RelichContents
PART III. APPLIED PRACTICAL MICROBIOLOGY
51. Mechanisms of Action of Antibacterial Agents
Ammara Mushtaq, Joseph Adrian L. Buensalido, Carmen E. DeMarco, Rimsha Sohail, and Stephen A. Lerner
52. Mechanisms of Action of Antifungal Agents
Jeffrey M. Rybak and P. David Rogers
53. Mechanisms of Action of Antiviral Agents
Guido Antonelli, Francesca Falasca, and Ombretta Turriziani
54. Phage Therapy: Bacteriophages as Natural, Self-Replicating Antimicrobials
Naomi Hoyle and Elizabeth Martin Kutter
55. Emergence of Antimicrobial Resistance in Hospitals
Paramita Basu, Joshua Garcia, and Priyank Kumar
56. Emerging Antimicrobial-Resistant Microorganisms in the Community
Negin Alidazeh Shaygh, Divya Sarvaiya, and Paramita Basu
57. Overview of Biofilms and Some Key Methods for Their Study
Paramita Basu, Michael Boadu, and Irvin N. Hirshfield
58. Biofilms in Healthcare
Rebecca K. Kavanagh, Arindam Mitra, and Paramita Basu
59. The Business of Microbiology
Michael C. Nugent and Lorrence H. Green
60. Launching a Microbiology-Based Company
Leonard Osser
61. Microbiology for Dental Hygienists
Victoria Benvenuto and Donna L Catapano
62. Microbiology for Pre-College Teachers
Madge Nanney and Scott Sowell
63. Microbiology for Home Inspectors
William E. Herrmann
Survey of Selected Clinical, Commercial, and Research-Model Eubacterial Species
Index
Biography
Edited by
Emanuel Goldman is a professor in the Department of Microbiology, Biochemistry, and Molecular Genetics of the New Jersey Medical School (NJMS), Rutgers Biomedical and Health Sciences (RBHS), a division of Rutgers University, Newark, New Jersey. He graduated with honors from the Bronx High School of Science in 1962, received a BA (cum laude) from Brandeis University in 1966, where he was a chemistry major and music minor, and completed his PhD in biochemistry at the Massachusetts Institute of Technology in 1972. He performed postdoctoral research at Harvard Medical School and at the University of California, Irvine, before joining the faculty of the New Jersey Medical School in 1979, where he rose through the ranks to professor in 1993. Among his awards and honors, Dr. Goldman was a Damon Runyon fellow, a Lievre senior fellow of the California Division, American Cancer Society, and a recipient of the Research Career Development Award from the National Cancer Institute.
Among his service activities, he was an officer and organizer of the New York–New Jersey Molecular Biology Club, served as a full member of an American Cancer Society Study Section, and continues to serve on the editorial boards of Protein Expression and Purification and Applied and Environmental Microbiology. He was also twice elected by his colleagues to serve as the president of his university’s chapter of the American Association of University Professors, and he was elected to serve as president of the Faculty Organization of NJMS. Among several areas of research activity, he has focused on the role of tRNA in the elongation of bacterial protein synthesis, including uncharged tRNA, codon bias, and programmed translational frameshifts. In addition to numerous scientific peer-reviewed publications and publications in the lay press, he has contributed a chapter to Zubay’s Biochemistry textbook and four chapters to the Encyclopedia of Life Sciences. His recently published Comment in Lancet, "Exaggerated risk of transmission of COVID-19 by Fomites", has attracted significant international attention.
Lorrence H. Green, Ph.D., President of Westbury Diagnostics, Inc. earned his PhD in Cell and Molecular Biology from Indiana University, Bloomington, Indiana, in 1978. He followed this with three years of recombinant DNA and genetic research at Harvard University. In 1981, he moved into Industry by joining Analytab Products Inc., a major manufacturer of in vitro diagnostic test kits. During the next twelve years he helped to invent and manufacture over 40 diagnostic test kits, and rose to become the Director of New Product Development and Product Support.
In 1993, Dr. Green founded Westbury Diagnostics, Inc., a microbiology-biotechnology based contract research and development laboratory also offering consulting services. Mixing his love of business with his love of teaching Dr. Green has served as an adjunct associate professor of microbiology at the NY College of Osteopathic Medicine, and is currently an Adjunct Assistant Professor of Biology at Farmingdale State College and a Director of the Fundamentals of the Bioscience Industry Program at Stony Brook University of the State University of New York.
Dr. Green is on the steering committee, and is a former Chairman, of the Microbiology Section of the NY Academy of Sciences. He was also the long time Treasurer of the NYC Branch of the ASM. From 2001 until 2004 he was a member of the Advisory Committee on Emerging Pathogens and Bioterrorism to the New York City Commissioner of Health. In 2013 he was appointed to the Board of Directors of the Long Island Advancement of Small Business.
His main interests involve using technology in the development of commercial products and in being an entrepreneur who invests in and develops companies. He enjoys providing mentorship and career advice to students at all levels. He has spoken at many career day events, judged many regional science fairs, and has helped dozens of young people with applications to medical school, nursing school, physician’s assistant school, and with starting companies. Most recently he has become involved in government and is currently the Chairman of the Town of Mamakating Planning Board.