1st Edition
Practical Transfusion Medicine for the Small Animal Practitioner
This guide to laboratory procedure in transfusions for dogs and cats provides easily accessible, clinical information regarding the collection, storage, and use of blood and blood products in the small animal patient. It includes step-by-step procedures for the separation of whole blood into components, along with a “how to” for establishing a community-based blood bank for dogs and cats. The immunologic and genetic principles are presented clearly for the practitioners and laboratorians who handle blood and blood products for the treatment of selected cancers and bleeding disorders in small animals. A practical section discusses the guidelines for Biosafety and Quality Assurance for a transfusion service.
Published by Teton New Media in the USA and distributed by CRC Press outside of North America.
Section1 Creation of a Community
Based Blood Bank
Introduction
Some Helpful Hints
Owner Recruitment
Owner and Donor Attributes
Basic Equipment Needs for Blood
Collection, Processing and Storage
Section 2 Blood Collection Systems,
Processing, Storage
and Shipment
Anticoagulants and Preservatives
Blood Collection Systems
Blood Product Overview
Blood Collection Systems and
Pre-Processing Guidelines
Preparation of Fresh Whole Blood
Preparation of Red Cells and
Fresh Frozen Plasma
Preparation of Cryoprecipitated
Anti-Hemophilic Factor and
Cryo-Poor Plasma
Preparation of Platelet Rich Plasma
Short Draw and Decreasing the
Amount of Anticoagulant Preservative
Purchasing and Receiving Blood Products
from Outside Sources
Preparing Blood Products for Shipment
Section3 Clinical Considerations in
Transfusion Practice
The Crossmatch
Blood Transfusion Guidelines
Blood Substitutes: Alternatives to
Blood and Blood Products
Compatible IV Solutions
Blood Administration Sets
Adverse Effects of Blood Transfusion
Section4 Biosafety
Laboratory Safety
Quality Assurance
Records
Inventory Management
Section5 Methods
Preparation of Fresh Whole Blood
Preparation of Red Cells and
Fresh Frozen Plasma
Preparation of Cryoprecipitate and
Cryoprecipitate-Poor Plasma
Preparation of Platelet Rich Plasma
Crossmatch Procedure
Washed Cell Suspension
Reaction Grading
Saline Replacement Procedure
Warming Whole Blood, Red Cells or
Thawing FFP, Cryo or Cryo-Poor Plasma
Centrifuge Calibration
Appendix 1 Manufacturers
of Blood Collection Bags
Appendix 2 Organizations
with Veterinary Blood Bank
Interests
Index
Recommended Readings
