Preservation of Human Oocytes: From Cryobiology Science to Clinical Applications, 1st Edition (Hardback) book cover

Preservation of Human Oocytes

From Cryobiology Science to Clinical Applications, 1st Edition

Edited by Andrea Borini, Giovanni Coticchio

CRC Press

306 pages | 40 B/W Illus.

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pub: 2009-08-27
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Oocyte cryopreservation entails important potential advantages for human

IVF, offering a less ethically disputable alternative to embryo cryopreservation,

simplifying and making safer oocyte donation, and giving an opportunity for

fertility preservation to women at risk of premature ovarian failure as an effect

of genetic factors or chemo- or radiotherapies. Oocyte cryopreservation could

also meet the expectations of women wishing to preserve their fertility for

social reasons. In the last few years, advances in cryopreservation

methodologies have dramatically improved the efficiency of oocyte

cryopreservation, leading to the birth of over a thousand babies and

challenging the supremacy of embryo cryopreservation as the preferred

form of fertility preservation. This text has been conceived with the aim of offering a comprehensive view of the state of the art of oocyte cryopreservation. It covers fundamental concepts of low temperature storage (controlled rate slow cooling and

vitrification), aspects of oocyte physiology relevant to the process of

cryopreservation, essential biological and clinical evidence, and ethical

implications of oocyte cryopreservation, thereby providing a complete

overview of progress in this strategy in assisted reproduction.

Table of Contents


A Historical Overview of Embryo and Oocyte Preservation in the World of Mammalian In Vitro Fertilization and Biotechnology

Nucharin Songsasen and Pierre Comizzoli

Principles of Cryopreservation

David E. Pegg

The Rational Basis for Controlled Rate Slow Cooling

Barry Fuller and Sharon Paynter

Fundamentals and Current Practice of Vitrification

Lilia L. Kuleshova

Predictive Models for the Development of Improved Cryopreservation Protocols for Human Oocytes

John McGrath


The Natural Life Cycle of the Mammalian Oocyte

David F. Albertini and S. Darlene Limback

Essential features of the mature oocyte

Ursula Eichenlaub-Ritter

Expression of Functional Aquaporins in Oocytes and Embryos and the Impact on Cryopreservation

François Chauvigné and Joan Cerdà

Oocyte Storage at Different Developmental Stages

Mafalda Rato and Carlos E. Plancha

Oocyte Cryopreservation in the Mouse

Maureen J. Wood

Oocyte Storage in Domestic Species

Gábor Vajta, Jason Conceicao, and John Yovich

Control of the Solution Effect During Controlled-Rate Cooling: Principles and Practical Application

James J. Stachecki

Cryopreservation and the Cytoskeleton of the Human Oocyte

Giovanni Coticchio and Maria Antonietta Bonu

Non-invasive Assessment of Cryopreserved Oocytes Through Polarized Light Microscopy

Markus Montag, Maria Köster, Benjamin Rösing, Katrin van der Ven, Hans van der Ven

Impact of Cryopreservation on Oocyte Physiology, Metabolism and the Proteome

Mark G. Larman and David K. Gardner


Quality Management of a Low Temperature Storage Program in the Human IVF Laboratory

Lucia De Santis

The Contribution of Embryo Cryopreservation to the Clinical Efficacy of ART and to a Reduction of ART Multiple Pregnancies

Karl G. Nygren

Factors Influencing the Clinical Efficiency of Oocyte Cryopreservation

Debra A. Gook and David H. Edgar

The Early and Recent History of Oocyte Cryopreservation in Human IVF

Eleonora Porcu, Leonardo Notarangelo, Antonia Bazzocchi, Chiara Landolfo, and Stefano Venturoli

Success Rates from Oocyte Cryopreservation

Andrea Borini and Maria Antonietta Bonu

Clinical Evidence of Oocyte Vitrification

Ri-Cheng Chian and Yin-Xia Cao

Oocyte Storage for Fertility Preservation Before Radio- and Chemotherapy

Michael von Wolff

The Impact of Oocyte Storage in Oocyte Donation Treatments

Ana Cobo and José Remohí

The Health of Children from Cryopreserved Oocytes and Embryos

Audrey Soo and Alastair Sutcliffe

The Ethics of Fertility Preservation

Carlo Flamigni

About the Series

Reproductive Medicine and Assisted Reproductive Techniques Series

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / General
MEDICAL / Reproductive Medicine & Technology