In the seven years since the publication of Principles and Practice of Bioanalysis bioanalytical methods have remained the same, but their usage patterns have changed. This second edition of a bestseller provides an updated guide to the techniques used in developing and running ultra-trace analyses for drugs, metabolites, and other substances.
Extensively revised, this volume reflects the dynamic growth in methodologies that has occurred over the past five years. Each chapter has been overhauled or re-written to reflect modern practice. Experts in the field offer a logical path through the problems of small molecule bioanalysis, enabling you to choose the appropriate methods of analysis across a number of fields, including the agrochemical and pharmaceutical industries. They cover a wide range of analytical methods, including mass spectrometry, liquid chromatography, immunoassay, NMR, and gas chromatography.
Presenting the information in an informal and highly accessible style, the book covers the theory and practice of bioanalysis, enabling you to gain maximum benefit and to optimize analytical methods. With practical advice and guidance, it delineates how to develop robust analytical methods for a range of compounds, both natural and synthetic. Using these insights, you can more easily recognize problems and quickly solve them.
Table of Contents
Physicochemical Properties of Drugs and Metabolites and Their Impact on Bioanalysis, H. Wiltshire and A. Nedderman
Sample Preparation, C.A. James
Basic HPLC Theory and Practice, A. Gray and P. Lawrence
HPLC Optimisation, D. Bakes
HPLC Detectors, K.C. Saunders and R.F. Venn
Gas Chromatography: What It Is and How We Use It, P. Andrew and M.V. Doig
Thin-Layer Chromatography, H. Wiltshire and A. Nedderman
Immunoassay Techniques, R.F. Venn
Automation of Sample Preparation, K.C. Saunders and C.A. James
Fundamental Aspects of Mass Spectrometry, M.V. Doig and D.M. Higton
Quantitative LC –MS, D.M. Higton and M.V. Doig
Mass Spectrometric Identification of Metabolites, G. Dear
Nuclear Magnetic Resonance In Drug Metabolism, P. Gilbert
Metabolite Isolation, G. Dear, A. Nedderman, and H. Wiltshire
Strategy for the Development of Quantitative Bioanalytical Procedures, D. Bakes Index
"The book consists of 15 chapters written by internationally distinguished experts from biotech and pharmacological companies, mostly located in United Kingdom…. Each chapter contains a bibliography of the most important papers in each field discussed in the book. This book is quite valuable theoretically and methodologically for specialists of various fields of analytical chemistry and biochemistry, molecular biology, and biotechnology. I believe that this book will become an important desk book for researchers working with bioanalysis of various drugs and metabolites."
—G. Ya. Wiederschain, Doctor of Biological Sciences, in Biochemistry, 2008, Vol. 73, No. 12
"… a living guide on the subject, scrutinizing how its topics have changed over the years – mass spectronomy, immunoassay, gas chromatography and more. A scholarly, seminal piece, Principles and Practices of Bioanalysis is highly recommended for community library science collections."
– James A. Cox and Diane C. Donovan in Midwest Book Review, 2008
"I found it instructive and interesting – an adjective that unfortunately can only be applied to textbooks relatively infrequently!"
– E. R. Adlard, in Chromatographia, July 2008, Vol. 68, No. 1/2