Producing Biomolecular Substances with Fermenters, Bioreactors, and Biomolecular Synthesizers: 1st Edition (Hardback) book cover

Producing Biomolecular Substances with Fermenters, Bioreactors, and Biomolecular Synthesizers

1st Edition

By William L. Hochfeld

CRC Press

408 pages | 61 B/W Illus.

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Description

Containing authoritative and in-depth coverage, Producing Biomolecular Materials Using Fermenters, Bioreactors, and Biomolecular Synthesizers examines the bioproduction systems that support the controlled, automated, and quantity growth of proteins. The book discusses the substance, character, makeup, and quality of the basic materials used in the production and downstream processing of boimolecular materials: raw materials, reagents, intermediates, and consumables.

Dr. Hochfield gets right to the point, explaining just what must be done and how to do it effectively, then providing the formula necessary for reaching the required value, allowing you to simply plug-in your data and make protein. However, if you actually do need the origin and derivation of any given formula, you can go right to the extensive reference section in the Appendix, find the formula you need in the exact form that you need it, without having to wade through numerous pages of extraneous material.

This classic work presents unparalled, detailed, and cutting-edge information on bioprocessing systems. A working reference and formulary for producing recombinant, bioactive, or other exotic proteins, peptides, and nucleic acids to specification, the text provides coverage of the related technologies, coupled with the extensive biotechnology glossary, manufacturer’s directories, extensive references, important formulae, charts, illustrations, comprehensive index, emphasis on practical techniques, time-proven methods, and essential applications. These features combine with its ingenious, easy-to-use layout to make it the resource you will consult on a regular basis.

Reviews

“This book is designed for biochemical  and biopharmaceutical engineers, technicians, scientists new to biotechnology, production staff, QA personnel, and managers. It is fully equipped with a biotechnology glossary, numerous references, and an exceptionally extensive index, and it emphasizes practical techniques, methods, and applications. This volume presents unparallel, detailed, and cutting-edge information on bioprocessing systems, and is a working reference and formulary for producing recombinant, bioactive, or other exotic proteins, peptides, and nucleic acids to specification. The text provides coverage of the related technologies, coupled with the extensive biotechnology glossary, manufacturer’s directories, extensive references, important formulae, charts, illustrations, comprehensive index, and emphasis on practical techniques, time-proven methods, and essential applications. These features combine with its easy-to-use layout making it an essential resource for all individuals involved in this interesting and fast developing area of bioscience.”

— John F. Kennedy, Bo Liu, Chembiotech Laboratories, Institute of Research and Development, University of Birmingham Research Park, UK, in International Journal of Biological Macromolecules, 2007

“This volume examines the bioproduction systems that support the controlled, automated, and quantity growth of proteins. The book discusses the substance, character, makeup, and quality of the basis materials used in the production and downstream processing of biomolecular materials: raw materials, reagents, intermediates, and consumables. … the text provides coverage of the related technologies, coupled with the extensive biotechnology glossary, manufacturer’s directories, extensive references, important formulae, charts, illustrations, comprehensive index, emphasis on practical techniques, time-proven methods, and essential applications. …”

— In Anticancer Research, 2006

“… effectively and extensively covers fermenters, bioreactors, and biomolecular synthesizers. It is must reading for those new to biopharmaceuticals and is a useful reference for the experienced. … The bibliography and list of recommended reading at the end of the book is very useful in making this book a single source for all the diverse information necessary for recombinant biotechnology fields. The price of the book is moderate and I strongly recommend this book for both academic libraries and for interdisciplinary courses. … can be used day to day by management, researchers, and plant operators. Many industries can use this book in their employee training programs. … the author must be congratulated for compiling a fine work on such a vast topic.”

— Milind Deshpande, Technical Director, Fermentation and Processing Center for Biocatalysis and Bioprocessing, University of Iowa

Table of Contents

Introduction

Biotechnology

Bioproduction

The Bioprocess

Cell Lines

Cell-Culture Process

Separation, Recovery, Purification

Virus and Foreign-DNA Removal

Quality Assurance

Technology Overview

Biomolecular Foundations

Cellular Variation

Key Molecular Interactions

Base-Pair Complementarity

Genetic Coding

Interrupting Gene Segments

Sequence Determinations

Cloning

Synthesized DNA

Cell Lines

Expression Systems

Introduction to the Bioprocess

Overview

Optimizing Product Yield

Kinetic Models

cGMP Standards

Contract Manufacturing

Complex Mixtures

Biomolecular Products

Joint Manufacturing

Downscaling to Scale Up

Large-Scale Bioprocesses

Scaling-Up In-House or in a Contract Facility

Lab-to-Pilot-Plant-to-Production

Increasing Bioprocess Scale

Differing Features at Larger Scale

Simulating Environment at Scale

Detailed Specifications

Impact on Downstream Bioprocessing

FDA Biologics Review Process

Regulating Biological Products

Recombinant DNA Materials and Methods

Cloning

Applications

Screening and Selection

DNA Synthesis

Cell Lines

Expression Systems

Vector Construction

Prokaryotic and Eukaryotic Growth Characteristics

Expression Levels

Intra- vs. Extra-cellular Expression

Intracellular Expression

Extracellular Expression

Glycosylation

Mammalian Cell Glycosylation

Insect Cell Glycosylation

General Considerations

Further Post-translational Modifications

Economic Concerns

Regulatory Concerns

Manufacturers’ Directory

Enzymes

Overview

Enzyme Protein Engineering

Transformation in Non-Aqueous Systems

Restriction Enzymes

Restriction-Enzyme Classes

Class I Restriction Enzymes

Class II Restriction Enzymes

Class III Restriction Enzymes

Restriction-Enzyme Specificity

Star Activity

Enzyme Families and Compatible Ends

Isoschizomers

Reverse Transcriptases

DNA and RNA Nucleases

Manufacturers’ Directory

DNA-Amplification Reagents

Overview

Amplification by DNA Synthesis (PCR)

Amplification by RNA Transcription (TAS and 3SR

Amplification by Ligation (LAR, LCR, LAS)

Amplification by RNA Replication (Q Replicase)

Sequence-Based Amplification

Manufacturers Directory

Cell-Culture Media

Overview

Prokaryotic Cell-Culture Medium

Eukaryotic Cell-Culture Medium

Defined Medium

Serum

Other Sera

Serum Contamination

Serum-Free (Defined) Medium

Cell-Culture Gels

Media Supplements

Attachment Factors

Buffers

Sodium

Earle’s Balanced Salt Solution

Growth Factors

Epidermal Growth Factor (EGF)

Fibroblast Growth Factors (FGF)

Acidic FGF

Basic FGF

Other Growth Factors

Lectins

Toxins

Transport Factors

Vitamins

Water

Manufacturers’ Directory

Fermenters

Introduction to Fermentation

Historical Perspective

The Fermentation Process

Enzyme Fermentation in the Organic Phase

Cell Growth and Production

Factors Affecting Specific Growth Rate

Kinetic Models

Optimal Conditions

Recombinant Culture Kinetics

Fermenter Design

Kinetic Modeling of the Fermentation Process

STF Batch Scale Up

Power Number Method for STF Geometric Scale Up

Continuous-Culture Scale Up

Fermenter Manufacturers Directory

Bioreactors

Introduction

Aseptic Conditions

Monoclonal Antibody Production

mAB Production in Ascites Fluid

Bioreactor Engineering and Design

Bioreactors II: Kinetic Modeling and Bioreactor Dynamics and Bioreactor Design Program

Bioreactor Manufacturers Directory

Biomolecular Synthesizers

DNA Synthesizers

Manufacturers Directory

Technology Overview

Peptides by Solid-Phase Synthesis

Manufacturers Directory

Biotechnology Glossary

References and Recommended Reading Index

Subject Categories

BISAC Subject Codes/Headings:
MED071000
MEDICAL / Pharmacology
SCI086000
SCIENCE / Life Sciences / General